Instrumental aspects of capillary supercritical fluid chromatography

Abstract

The bask Instrument components requlred for caplllary supercrltlcal fluld chromatography include a high-pressure pump with pressure programmer, a small-volume sample Inlet system, a constant temperature oven, and a small-volume detector. The major stresses applied to the hstrumentatlon are the hlgh pressures and temperatures required to maintain the mobile phase at or above Its crltlcal polnt. Of the dlfferent sample introductlon systems studied, spllt Injection presently appears to be the most effectlve. By use of the described hstrumentatlon, a plate helght of 0.30 mm was obtalned for pyrene ( k = 0.50) on a 100-hm caplllary column contalnlng a bonded poly(methylphenylslloxane) statlonary phase.

Modified flame ionization detector for the analysis of large molecular weight polar compounds by capillary supercritical fluid chromatography

“…The chromatographic system is similar to that previously described [2,4]. The mobile phase (C02) was delivered by a Varian 8500 syringe pump modified for pressure control and the density of the fluid was regulated using a microcomputer as described earlier [4].…”

Theory and Practice of UHPLC and UHPLC–MS

“…In the 1980s, Novotny and Lee (Peaden and Lee, 1983;Peaden et al, 1982;Springston and Novotny, 1981;Novotny et al, 1981) introduced the new concept of open tubular capillary column SFC (cSFC). A typical capillary column was a fused silica capillary coated with a polymer, such as dimethyl polysiloxane stationary phase.…”

Evolution and Current Trends in Liquid and Supercritical Fluid Chromatography

“…Despite the outstanding physicochemical properties of supercritical fluids, SFC development was only anecdotic during its first two decades, mostly because of the significant interest of the chromatographic community for the already well-established gas chromatography (GC) and the first and promising developments of high performance liquid chromatography (HPLC). Real SFC breakthrough only occurs in the early 80's thanks to the original publications of Novotny et al [214][215][216]. Their work, together with the successful studies led by Lee and co-workers have strongly contributed to the popularization of SFC using capillary or open tubular stationary phases [215,[217][218][219].…”

“…Real SFC breakthrough only occurs in the early 80's thanks to the original publications of Novotny et al [214][215][216]. Their work, together with the successful studies led by Lee and co-workers have strongly contributed to the popularization of SFC using capillary or open tubular stationary phases [215,[217][218][219]. In the early years, capillary SFC (cSFC) was also strongly promoted by GC users and was mostly considered as an elegant extension of GC, allowing an expansion of the solubility domain of classical GC [220,221].…”