Insights of Bipolaris sorokiniana secretome - an in silico approach

Pathak, Gauri; Gurjar, Gayatri; Kadoo, Narendra Y.

doi:10.2478/s11756-020-00537-4

Cited by 7 publications

(4 citation statements)

References 88 publications

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“…There were significantly more secreted proteins than other pathogens of the same genus, and the functions of these secreted proteins have not been reported (Condon et al, 2013 ). Pathak et al ( 2020 ) investigated the secretome of 196 proteins predicted to be present in B. sorokiniana in silico. The ToxA gene encodes a host‐selective toxin (HST) that functions as an effector, and B. sorokiniana has been shown to carry this gene (Sudhir et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

A novel effector, CsSp1, from Bipolaris sorokiniana, is essential for colonization in wheat and is also involved in triggering host immunity

Zhang

Wang

et al. 2021

Molecular Plant Pathology

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The hemibiotrophic pathogen Bipolaris sorokiniana causes root rot, leaf blotching, and black embryos in wheat and barley worldwide, resulting in significant yield and quality reductions. However, the mechanism underlying the host-pathogen interactions between B. sorokiniana and wheat or barley remains unknown. The B. sorokiniana genome encodes a large number of uncharacterized putative effector proteins. In this study, we identified a putative secreted protein, CsSp1, with a classic N-terminal signal peptide, that is induced during early infection. A split-marker approach was used to knock out CsSP1 in the Lankao 9-3 strain. Compared with the wild type, the deletion mutant ∆Cssp1 displayed less radial growth on potato dextrose agar plates and produced fewer spores, and complementary transformation completely restored the phenotype of the deletion mutant to that of the wild type. The pathogenicity of the deletion mutant in wheat was attenuated even though appressoria still penetrated the host. Additionally, the infectious hyphae in the deletion mutant became swollen and exhibited reduced growth in plant cells. The signal peptide of CsSp1 was functionally verified through a yeast YTK12 secretion system. Transient expression of CsSp1 in Nicotiana benthamiana inhibited lesion formation caused by Phytophthora capsici. Moreover, CsSp1 localized in the nucleus and cytoplasm of plant cells. In B. sorokiniana-infected wheat leaves, the salicylic acid-regulated genes TaPAL, TaPR1, and TaPR2 were down-regulated in the ∆Cssp1 strain compared with the wild-type strain under the same conditions. Therefore, CsSp1 is a virulence effector and is involved in triggering host immunity.

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Section: Introductionmentioning

confidence: 99%

A novel effector, CsSp1, from Bipolaris sorokiniana, is essential for colonization in wheat and is also involved in triggering host immunity

Zhang

Wang

et al. 2021

Molecular Plant Pathology

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“…Bipolaris sorokiniana is the pathogen responsible for spot blotch disease in cereals. The disease could be carried out by seeds, roots, or air (Matorin et al, 2018;Pathak et al, 2020;Al-Sadi, 2021). Plowing, removing weeds and cereals, and introducing plants that are not affected by root rot into crop rotation reduces the amount of Bipolaris sorokiniana inoculum in the soil (Duveiller and Dubin, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…The fungus B. sorokiniana has a wide geographical distribution area (Guest, 2017;McDonald et al, 2018;Özer et al, 2020). The low yield of barley in Kazakhstan is largely due to the susceptibility of the cultivated varieties to leaf spotting, while the yield and productivity of plants are significantly reduced by 30-40% (Cegiełko et al, 2018(Cegiełko et al, , 2019Haas et al, 2018;Pathak et al, 2020). Barley strips are covered with dark spots with a brown or dark gray coating, photosynthesis is disrupted, the growth and development of plants slow down and they form fewer primary and secondary roots.…”

Section: Introductionmentioning

confidence: 99%

“…In Kazakhstan, studies on soil pathogens have recently been conducted to assess the collection of synthetic wheat for disease resistance (Suleimanova et al, 2016;Kuresbek et al, 2017;Morgounov et al, 2018) and the compatibility of barley varieties with leaf spot pathogens on an artificial infectious background (Pathak et al, 2020). The International Maize and Wheat Improvement Center (CIMMYT) researchers have conducted 2 intensive surveys for soil-borne diseases of grain crops and found that B. sorokiniana was the most common pathogen (Göksel et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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The Ability of Bipolaris Sorokiniana Isolated from Spring Barley Leaves to Survive in Plant Residuals of Different Crops

Dutbayev¹,

Kharipzhanova²,

Sultanova³

et al. 2022

OnLine Journal of Biological Sciences

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Spring barley is an important high-value crop globally.However, it is highly susceptible to soil-borne diseases in Kazakhstan and the world. Bipolaris sorokiniana (Sacc.) Shoemaker is the most important soil-borne pathogen that causes root rot and spot blotch diseases in cereal crops. This study aimed to evaluate the ability of B. sorokiniana isolated from spring barley leaves to survive in plant residuals of different crops. The fungus was isolated from spring barley leaves from the Zholbarys agro farm of Kerbylak district of Almaty, southeastern Kazakhstan, in 2021. Laboratory experiments were conducted at Kazakh National Agrarian Research University. The study was conducted on 10 th days of seedlings of cereal, legume, and oilseed crops. The obtained results showed that seedlings of spring barley, spring wheat, and oats retained the B. sorokiniana infection. Interestingly, the infection of B. sorokiniana isolated from leaves of spring barley statistically significantly increased symptoms of spot blotch on spring wheat and spring barley seedlings, while symptoms decreased on oats (P-value <0.01). The introduction of rapeseed, chickpeas, peas, and oats into the crop rotation system in southeastern Kazakhstan could limit the volume of the B. sorokiniana infection in spring wheat and spring barley. On rapeseed, peas, and chickpeas seedlings no symptoms of spot blotch were observed (P-value <0.01).

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Structural and catalytic advancement of fungal tannase: A proteomic contribution in industrial applicability

Biswas

Mitra

Mohapatra

2022

Bioresource Technology Reports

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Insights of Bipolaris sorokiniana secretome - an in silico approach

Cited by 7 publications

References 88 publications

A novel effector, CsSp1, from Bipolaris sorokiniana, is essential for colonization in wheat and is also involved in triggering host immunity

A novel effector, CsSp1, from Bipolaris sorokiniana, is essential for colonization in wheat and is also involved in triggering host immunity

The Ability of Bipolaris Sorokiniana Isolated from Spring Barley Leaves to Survive in Plant Residuals of Different Crops

Structural and catalytic advancement of fungal tannase: A proteomic contribution in industrial applicability

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