Insights into the slurry packing and bed structure of capillary liquid chromatographic columns

Shelly, Dennis C.; Antonucci, Vincent; Edkins, Thomas J.; Dalton, T.

doi:10.1016/s0021-9673(00)90570-3

Cited by 19 publications

(10 citation statements)

References 14 publications

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“…The presence of a constant packing rate has been found to be crucial for producing a packed bed with longitudinal uniformity [9]. While a constant packing rate is targeted, it is common for the initial bed formed to pack more quickly due to the absence of a large length of packed bed producing elevated flow resistance.…”

Section: Role Of Column Packing On Performancementioning

confidence: 99%

1.1 μm Superficially porous particles for liquid chromatography

Blue

Jorgenson

2015

Journal of Chromatography A

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Section: Role Of Column Packing On Performancementioning

confidence: 99%

1.1 μm Superficially porous particles for liquid chromatography

Blue

Jorgenson

2015

Journal of Chromatography A

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“…Some research groups have tried to develop a better understanding of the packing processesincorporating colloid chemical studies, filtration theories and stress behavior during bed compression [69,[87][88][89][90]. Although certain trends could be assigned with these studies, a sound theoretical description and understanding of packing methods for microcolumns is not available yet.…”

Section: Column Developmentsmentioning

confidence: 99%

Microcolumn liquid chromatography: instrumentation, detection and applications

Vissers¹,

Claessens

Cramers

1997

Journal of Chromatography A

208

101

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This review discusses many different aspects of microcolumn liquid chromatography (LC) and reflects the areas of microcolumn LC research interest over the past decades. A brief theoretical discussion on a number of major issues, such as column characterisation, chromatographic dilution effects and extracolumn band broadening in microcolumn LC is given. Recent progress in column technology and the demands and developments of instrumentation and accessories for microcolumn LC are also reviewed. Besides that, the developments in a large number of established and also more recent detection techniques for microcolumn LC are also discussed. The potential of hyphenation of microcolumn LC with other techniques, more particularly of multidimensional chromatography and microcolumn LC coupled to mass spectrometry is reviewed. Finally, the perspective of microcolumn LC separation methods is stressed by a number of relevant applications.

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“…To improve the packed bed structure, the column can be held under pressure for a prolonged period of time. In different variants of the slurry technique, the column is subjected to different pressure conditions during the packing process, such as holding the column either at constant pressure or programming the pressure to a higher final value [21]. In both cases the column is held at the final pressure for a long period of time.…”

Section: Resultsmentioning

confidence: 99%

“…Also, the slurry liquid should not react with the packing material and it should be easily washed out of the packed bed. Shelly et al [21] reported a comprehensive approach involving colloid chemistry, fluid dynamics, and packing rheology to study the dynamics of slurry packing.…”

Section: Introductionmentioning

confidence: 99%

Preparation of long packed capillary columns using carbon dioxide slurries

Malik

Lee

1993

J Microcolumn Separations

109

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Abstract. A new, reproducible method is described for the preparation of highly efficient fused silica packed capillary columns using pressure programmed liquid or supercritical carbon dioxide to carry the packing material into the capillary. The method allows facile preparation of long, uniformly packed capillary columns capable of producing over 240,000 theoretical plates in SFC. Columns of up to 10 meters in length were prepared using LC packing materials of different chemical nature and particle size. The new method does not require a separate step for preparing the slurry, and it is free from the drawbacks inherent in conventional slurry-packing methods. The lower viscosity and surface tension of carbon dioxide compared to conventional slurry-forming liquids, as well as the use of ultrasonic vibration and pressure programming in the packing process, provide favorable conditions for achieving packing uniformity over greater column lengths than is achievable by other packing techniques. Proper restrictors and appropriate decompression rates during the use of these columns in SFC were found to be very important in achieving optimum column performance. In comparison to the use of open tubular columns, higher efficiencies, greater sample capacities, and faster analysis speeds can be obtained. These advantageous features make the newly developed columns suitable for trace analysis, separation of very complex mixtures, and analysis of difficult-toseparate solutes.

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Insights into the slurry packing and bed structure of capillary liquid chromatographic columns

Cited by 19 publications

References 14 publications

1.1 μm Superficially porous particles for liquid chromatography

1.1 μm Superficially porous particles for liquid chromatography

Microcolumn liquid chromatography: instrumentation, detection and applications

Preparation of long packed capillary columns using carbon dioxide slurries

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