Insights into the microbiota of larval and postlarval Pacific white shrimp (Penaeus vannamei) along early developmental stages: a case in pond level

Wang, Hailiang; Huang, Jie; Wang, Ping; Li, Ting

doi:10.1007/s00438-020-01717-2

Cited by 17 publications

(24 citation statements)

References 34 publications

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“…Vibrios are also known to express genes involved in the metabolic degradation of chitin ( Hunt et al, 2008 ), an important component of the exoskeleton of the nauplii. As mouth opening occurs after the nauplius stage in Litopenaeus shrimps ( Wang et al., 2020a ; Wang et al., 2020b ), the vitellus is the only nutrient and energy resource until the zoae stage ( Harrison, 1997 ). Thus, these bacterial communities may be less represented in water and could find favorable organic carbon sources and growth conditions on the external layer of the eggs and the exoskeleton of the nauplii ( Hansen & Olafsen, 1999 ).…”

Section: Discussionmentioning

confidence: 99%

“…Bacterial communities specifically associated with the egg samples ( Fig. 5B ) were dominated by Pseudomonas , Acinetobacter and Labrenzia , 3 genera found in marine environments ( Fournier & Richet, 2006 ; Palleroni, 2015 ; Raj Sharma et al, 2019 ) and which had already been studied during the development of L. vannamei and P. monodon ( Rungrassamee et al, 2013 ; Wang et al., 2020a ; Wang et al., 2020b ). The egg samples were also composed of Corynebacterium , a genera isolated from coral mucus ( Ben-Dov et al, 2009 ).…”

Section: Discussionmentioning

confidence: 99%

“…Marinobacter and Thalassolituus have been isolated in farmed shrimp experiments ( Dineshkumar et al, 2014 ). Thalassolituus has also been identified at the nauplius stage of L. vannamei and has been suggested as a beneficial bacterium for larval development ( Wang et al., 2020a ; Wang et al., 2020b ). Vibrio and Thalassolituus have also been identified in healthy adult shrimps ( Rungrassamee et al, 2016 ; Wang et al., 2020a ; Wang et al., 2020b ) supporting our previous theory that some bacterial communities may be acquired from the breeders and kept throughout the whole lifecycle.…”

Section: Discussionmentioning

confidence: 99%

“…Marinobacter and Aestuariibacter have been identified in the rearing environment of adult L. vannamei and brackishwater shrimps ( Chen et al, 2019 ; Dineshkumar et al, 2014 ). Vibrio , Alteromonas and Thalassotalea have all been spotted in L. vannamei at different developmental stages as well as in the surrounding water ( Wang et al., 2020a ; Wang et al., 2020b ; Zhang et al, 2014 ; Zheng et al, 2017 ). In our study, these genera were highlighted from different ASVs in different compartments, suggesting that some taxa may be acquired from the aquatic environment by the eggs and the nauplii but also by the breeders which then potentially transmit them to their offspring, supporting the hypothesis of a possible vertical transmission of bacterial communities in L. stylirostris shrimp larvae ( Fig.…”

Section: Discussionmentioning

confidence: 99%

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Potential lineage transmission within the active microbiota of the eggs and the nauplii of the shrimp Litopenaeus stylirostris: possible influence of the rearing water and more

Giraud

Callac

Beauvais

et al. 2021

PeerJ

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Background Microbial communities associated with animals are known to be key elements in the development of their hosts. In marine environments, these communities are largely under the influence of the surrounding water. In aquaculture, understanding the interactions existing between the microbiotas of farmed species and their rearing environment could help establish precise bacterial management. Method In light of these facts, we studied the active microbial communities associated with the eggs and the nauplii of the Pacific blue shrimp (Litopenaeus stylirostris) and their rearing water. All samples were collected in September 2018, November 2018 and February 2019. After RNA extractions, two distinct Illumina HiSeq sequencings were performed. Due to different sequencing depths and in order to compare samples, data were normalized using the Count Per Million method. Results We found a core microbiota made of taxa related to Aestuariibacter, Alteromonas, Vibrio, SAR11, HIMB11, AEGEAN 169 marine group and Candidatus Endobugula associated with all the samples indicating that these bacterial communities could be transferred from the water to the animals. We also highlighted specific bacterial taxa in the eggs and the nauplii affiliated to Pseudomonas, Corynebacterium, Acinetobacter, Labrenzia, Rothia, Thalassolituus, Marinobacter, Aureispira, Oleiphilus, Profundimonas and Marinobacterium genera suggesting a possible prokaryotic vertical transmission from the breeders to their offspring. This study is the first to focus on the active microbiota associated with early developmental stages of a farmed shrimp species and could serve as a basis to comprehend the microbial interactions involved throughout the whole rearing process.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Potential lineage transmission within the active microbiota of the eggs and the nauplii of the shrimp Litopenaeus stylirostris: possible influence of the rearing water and more

Giraud

Callac

Beauvais

et al. 2021

PeerJ

View full text Add to dashboard Cite

show abstract

“…Information on microbiota provides potential application possibilities to improve the production of food animals. Recent studies have reported the formation of microbiota, which get stabilized by post larval stages (PL5-PL15), indicating the role of early feeding, food and feeding habits, feed additives in shaping the gut microbiome in penaeid shrimp P. monodon [9] and P. vannamei [12]. Recently, we have reported the gut microbial composition in adult P. indicus obtained from wild and farmed environments [13].…”

Section: Introductionmentioning

confidence: 92%

Microbial community composition associated with early developmental stages of the Indian white shrimp, Penaeus indicus

et al. 2022

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Gut microbiota is known to in uence the physiology, health, nutrient absorption, reproduction, and other metabolic activities of aquatic organisms. Microbial composition can in uence intestinal immunity and are considered as health indicators. Information on gut microbial composition provides potential application possibilities to improve shrimp health and production. In the absence of such information for Penaeus indicus, the present study reports the microbial community structure associated with its early developmental stages. Bacterial community associated with the early developmental stages (egg, nauplii, zoea, mysis, postlarvae-1, postlarvae-6 and postlarvae-12) from two hatchery cycles were analysed employing 16S rRNA high throughput sequencing. Proteobacteria and Bacteroidetes, were the two dominant phyla in P. indicus development stages. Sequential sampling revealed the constant change in the bacterial composition at genus level. Alteromonas was dominant in egg and nauplii stage, whilst Ascidiaceihabitans (formerly Roseobacter) was the dominant genera in both PL6 and PL12. The bacterial composition was highly dynamic in early stages and our study suggests that the mysis stage is the critical phase in transforming the microbial composition and it gets stabalised by early post larval stages. This is the rst report on the composition of microbiota in early developmental stages of P. indicus. Based on these results the formation of microbial composition seems to be in uenced by feeding at early stages. The study provides valuable information to device intervention strategies for healthy seed production.

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Microbiome analysis reveals the intestinal microbiota characteristics and potential impact of Procambarus clarkii

Xu,

Li,

Zhang

et al. 2024

Appl Microbiol Biotechnol

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The intestinal microbiota interacts with the host and plays an important role in the immune response, digestive physiology, and regulation of body functions. In addition, it is also well documented that the intestinal microbiota of aquatic animals are closely related to their growth rate. However, whether it resulted in different sizes of crayfish in the rice-crayfish coculture model remained vague. Here, we analyzed the intestinal microbiota characteristics of crayfish of three sizes in the same typical rice-crayfish coculture field by high-throughput sequencing technology combined with quantitative real-time polymerase chain reaction (qRT-PCR) and enzyme activity, investigating the relationship between intestinal microbiota in crayfish and water and sediments. The results showed that the dominant intestinal microbiota of crayfish was significantly different between the large size group (BS), normal size group (NS), and small size group (SS), where Bacteroides and Candidatus_Bacilloplasma contributed to the growth of crayfish by facilitating food digestion through cellulolysis, which might be one of the potential factors affecting the difference in sizes. Follow-up experiments confirmed that the activity of lipase (LPS) and protease was higher in BS, and the relative expression of development-related genes, including alpha-amylase (α-AMY), myocyte-specific enhancer factor 2a (MEF2a), glutathione reductase (GR), chitinase (CHI), and ecdysone receptor (EcR), in BS was significantly higher than that in SS. These findings revealed the intestinal microbiota characteristics of crayfish of different sizes and their potential impact on growth, which is valuable for managing and manipulating the intestinal microbiota in crayfish to achieve high productivity in practice. Key points • Significant differences in the dominant microflora of BS, NS, and SS in crayfish. • Cellulolysis might be a potential factor affecting different sizes in crayfish. • Adding Bacteroides and Candidatus_Bacilloplasma helped the growth of crayfish.

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Insights into the microbiota of larval and postlarval Pacific white shrimp (Penaeus vannamei) along early developmental stages: a case in pond level

Cited by 17 publications

References 34 publications

Potential lineage transmission within the active microbiota of the eggs and the nauplii of the shrimp Litopenaeus stylirostris: possible influence of the rearing water and more

Potential lineage transmission within the active microbiota of the eggs and the nauplii of the shrimp Litopenaeus stylirostris: possible influence of the rearing water and more

Microbial community composition associated with early developmental stages of the Indian white shrimp, Penaeus indicus

Microbiome analysis reveals the intestinal microbiota characteristics and potential impact of Procambarus clarkii

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