Microbial community composition associated with early developmental stages of the Indian white shrimp, Penaeus indicus

Abstract

Gut microbiota is known to in uence the physiology, health, nutrient absorption, reproduction, and other metabolic activities of aquatic organisms. Microbial composition can in uence intestinal immunity and are considered as health indicators. Information on gut microbial composition provides potential application possibilities to improve shrimp health and production. In the absence of such information for Penaeus indicus, the present study reports the microbial community structure associated with its early developmental stages. Bacterial community associated with the early developmental stages (egg, nauplii, zoea, mysis, postlarvae-1, postlarvae-6 and postlarvae-12) from two hatchery cycles were analysed employing 16S rRNA high throughput sequencing. Proteobacteria and Bacteroidetes, were the two dominant phyla in P. indicus development stages. Sequential sampling revealed the constant change in the bacterial composition at genus level. Alteromonas was dominant in egg and nauplii stage, whilst Ascidiaceihabitans (formerly Roseobacter) was the dominant genera in both PL6 and PL12. The bacterial composition was highly dynamic in early stages and our study suggests that the mysis stage is the critical phase in transforming the microbial composition and it gets stabalised by early post larval stages. This is the rst report on the composition of microbiota in early developmental stages of P. indicus. Based on these results the formation of microbial composition seems to be in uenced by feeding at early stages. The study provides valuable information to device intervention strategies for healthy seed production.

Active Microbiota of Penaeus stylirostris Larvae: Partially Shaped via Vertical and Horizontal Transmissions and Larval Ontogeny

“…A clear dynamic of the active microbial diversity associated with the larvae was evidenced throughout the whole rearing (Figures 3 and 4), as previously shown for the microbial diversity of other penaeids shrimp larvae: Penaeus indicus [46] or P. vannamei [3,47,48]. Strikingly different microbial compositions were observed between D1 (nauplii) and D2 (zoea), between D5 (zoea) and D7 (mysis) with a transition on D6 (zoea), along with the three distinct clusters underlined by the NMDS (Figure 3), regrouping larvae microbiota samples according to the larval stage.…”

AI-2/Lux-S Quorum Sensing of Lactobacillus plantarum SS-128 Prolongs the Shelf Life of Shrimp (Litopenaeus vannamei): From Myofibril Simulation to Practical Application

“…Vibrio and Psychrobacter were barely detected in Group L. SS-128, while both were detected in the control group (Vibrio: 0.72%, 0.72%, 1.53%; Psychrobacter : 0.50%, 0.28%, 0.50%) and luxS -mutant group (Vibrio: 1.31%, 0.29%, 0.47%; Psychrobacter : 1.11%, 0.90%, 0.90%). The study by Vinay et al (2022) showed that Proteobacteria is of the most abundant in all of the developmental stages [ 48 ]. Actinobacteria , Proteobacteria , and Bacteroidota are reported to be the largest relative abundance of bacterial phyla of Pacific White shrimp Penaeus vannamei during an outbreak of white feces disease [ 49 ].…”

Effects of dietary yeast culture on health status in digestive tract of juvenile Pacific white shrimp Litopenaeus Vannamei

“…As mentioned above, some microbiotas are helpful to digestion, absorption and utilization of nutrient, and the regulation of immune system, whose composition has an important impact on intestinal health [61] . Some studies indicated that the predominant bacterial phyla in shrimp digestive tract were Firmicutes, Proteobacteria and Bacteroidetes [ 13 , 62 , 63 ]. In the present study, Firmicutes, Proteobacteria and Bacteroidetes were the predominant bacterial phyla in the digestive tract of juvenile shrimp in all treatments, while at genus level, the bacterial composition of all the treatment were dominated by Muribaculaceael, Lactobacillus and Bacteroides .…”