Insights into the evolution of
            <i>Yersinia pestis</i>
            through whole-genome comparison with
            <i>Yersinia pseudotuberculosis</i>

Chain, Patrick; Carniel, Élisabeth; Larimer, Frank W.; Lamerdin, Jane E.; Stoutland, Page O.; Regala, Warren; Georgescu, Anca Meda; Vergez, Lisa M.; Land, Miriam; Motin, Vladimir L.; Brubaker, Robert R.; Fowler, Janet M.; Hinnebusch, J; Marceau, Michaël; Médigue, Claudine; Simonet, Michel; Chenal-Francisque, Viviane; Souza, Brian; Dacheux, Denis; Elliott, Jeffrey M.; Derbise, Anne; Hauser, Loren; García, Emilio García

doi:10.1073/pnas.0404012101

Cited by 580 publications

(594 citation statements)

References 46 publications

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“…This is similar to the Y. pseudotuberculosis iucA-D-iutA loci in strains IP32953 (Chain et al, 2004) and PB1 except for a Y225H change in the Y. pestis locus. Consequently, we altered this residue to Y225 in pAeroYp5.…”

Section: Y Pestis Utilizes Exogenous Aerobactin As An Iron Sourcesupporting

confidence: 51%

“…PB1-2046.1/0 is a derivative of the sequenced PB1 strain that has a kan insertion in irp2, making it unable to synthesize the yersiniabactin siderophore . The genome sequence of strain PB1 shows an iucA-D-iutA locus with no obvious defects and is identical to the same locus in strain IP32953 (Chain et al, 2004). Despite this, the PB1-2046.1/0 supernatants did not support the growth of E. coli LG1522, indicating that Y. pseudotuberculosis is unable to synthesize aerobactin.…”

Section: Y Pestis Utilizes Exogenous Aerobactin As An Iron Sourcementioning

confidence: 95%

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Analysis of the aerobactin and ferric hydroxamate uptake systems of Yersinia pestis

et al. 2007

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Yersinia pestis genomes contain genes homologous to the aerobactin receptor (iutA) and biosynthetic genes (iucABCD) as well as the ferric hydroxamate uptake system (fhuCDB) of Escherichia coli. However, iucA is disrupted by a frameshift mutation. An E. coli strain carrying the cloned Y. pestis aerobactin region was unable to produce aerobactin, but could use the siderophore as an iron source. Repair of the frameshift mutation in iucA did not allow aerobactin production in E. coli or Y. pestis. In contrast, a Y. pestis strain with a plasmid encoding the iucABCD-iutA genes from Shigella flexneri or pColV-K30 did produce and secrete the siderophore. In addition, Yersinia pseudotuberculosis PB1, which encodes the iucABCD-iutA locus without the Y. pestis-specific frameshift mutation, also failed to produce aerobactin. The Y. pestis fhuCDB operon, encoding an ABC transporter for a range of hydroxamate siderophores, was able to complement a strain of E. coli with a transposon insertion in fhuC, allowing utilization of aerobactin and ferrichrome. Y. pestis KIM6, a strain deficient in the production of the siderophore yersiniabactin, was able to use both the ferrichrome and the aerobactin siderophores as a source of iron. Mutations in iutA or the fhu operon abolished the ability of KIM6 to use aerobactin. Mutations in the fhu operon, but not in iutA, affected the ability of KIM6 to use ferrichrome. This demonstrates that Y. pestis uses both ferrichrome and aerobactin, but has lost the ability to synthesize aerobactin.

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Section: Y Pestis Utilizes Exogenous Aerobactin As An Iron Sourcesupporting

confidence: 51%

Section: Y Pestis Utilizes Exogenous Aerobactin As An Iron Sourcementioning

confidence: 95%

Analysis of the aerobactin and ferric hydroxamate uptake systems of Yersinia pestis

et al. 2007

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“…Population-genetics studies have suggested that Y. pestis recently emerged from Y. pseudotuberculosis (1500 to 20 000 years ago) and thus these bacteria are highly similar at the genomic level (Achtman et al, 1999). However, genomic sequencing of these species has revealed that Y. pestis, despite being highly virulent, is in the early stages of genome decay, with up to 10 % of its genes represented as pseudogenes and presumably non-functional (Parkhill et al, 2001;Chain et al 2004;Lerat & Ochman, 2005). Amongst these genes are several known to be important in the enteric life style of Y. pseudotuberculosis, including genes for flagella, invasin, urease and cytotoxic necrotizing factor.…”

Section: Introductionmentioning

confidence: 99%

The importance of the Rcs phosphorelay in the survival and pathogenesis of the enteropathogenic yersiniae

et al. 2008

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“…Since then, several orthologs have been found in Pseudomonas aeruginosa (3), Pseudomonas fluorescens (4), Yersinia pestis (5), Yersinia enterocolytica (Sanger Institute) and Yersinia pseudotuberculosis (6). HasA (heme acquisition system) hemophores form an independent family of highly conserved hemecarrier proteins that are not homologous to any other known proteins.…”

mentioning

confidence: 99%

Deciphering the Structural Role of Histidine 83 for Heme Binding in Hemophore HasA

Caillet‐Saguy

Turano

Piccioli

et al. 2008

Journal of Biological Chemistry

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Insights into the evolution of Yersinia pestis through whole-genome comparison with Yersinia pseudotuberculosis

Cited by 580 publications

References 46 publications

Analysis of the aerobactin and ferric hydroxamate uptake systems of Yersinia pestis

Analysis of the aerobactin and ferric hydroxamate uptake systems of Yersinia pestis

The importance of the Rcs phosphorelay in the survival and pathogenesis of the enteropathogenic yersiniae

Deciphering the Structural Role of Histidine 83 for Heme Binding in Hemophore HasA

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