Insights into pathological and molecular characterization of avipoxviruses circulating in Egypt

Lebdah, M.; Ali, A; Ali, Ahmad; Hassanin, Ola

doi:10.1080/00071668.2019.1639141

Cited by 7 publications

(19 citation statements)

References 21 publications

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“…The successful propagation on CAM of ECE resulted in 130 positive samples with induction of pocks on the CAM with various morphological characteristics and variable degrees of membrane thickening. The isolated viruses revealed two distinct CAM phenotypes, the first one, is the classical pock shape, small to large in size, white to yellow in color and the second phenotype, is abundant white to yellow thickening of different sizes of CAMs [23].…”

Section: Virologymentioning

confidence: 99%

“…Several studies based mainly on histopathology for the diagnosis of APV infection in different bird species. Those studies reported that the most characteristic features of infection, either the lesion is cutaneous, diphtheritic or from infected CAM, are hyperplasia of the epithelium and enlargement of cells, with associated inflammatory changes [3,22,23]. Abdallah and Hassanin [22] studied the histopathological changes of the cutaneous lesions of 6 APV clinical cases which revealed ballooning degeneration of the keratinocytes having large eosinophilic intracytoplasmic inclusions with central pale zone (Bollinger bodies).…”

Section: Histopathologymentioning

confidence: 99%

“…Moreover, the histopathological examination of the CAM sections showed a hyperplastic ectodermal layer which suffered from focal destruction with high infiltration of inflammatory cells with the presence of elementary or eosinophilic intracytoplasmic inclusion bodies. Frequently, mesodermal reaction was seen with intense clusters of inflammatory cells around infected mesoderm epithelial nests [23].…”

Section: Histopathologymentioning

confidence: 99%

“…Polymerase chain reaction (PCR) is one of the most recent, useful and delicate diagnostic techniques used for detection and characterization of APVs. Avipoxvirus genomic DNA sequences of different sizes can be amplified by PCR using specific primers designated previously [3,4,22,23]. This technique is helpful when a very small amount of virus is present in the sample and in case of mixed infections as fragments of different sizes could be amplified in single PCR reaction using pathogen-specific primers.…”

Section: Molecular Biology (Genes Clades and Subclades)mentioning

confidence: 99%

“…The turkey isolates, Egypt_FWPV_TK1, TK2 and TK4, were divergent sequences and were branched into the novel subclade A1.b. The 4 PGPV strains were clustered together into subclade A2.c [23].…”

Section: Molecular Biology (Genes Clades and Subclades)mentioning

confidence: 99%

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Avipoxvirus in Egypt and African continent: A review

Lebdah

Hassanin

Ali

2019

Zagazig Veterinary Journal

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Fowl pox disease is a slow-spreading viral infection of wild and domesticated birds of both genders, all ages and breeds. The disease occurs in two distinct forms; the more common cutaneous or dry form and the less common diphtheritic form. Fowl poxvirus (FWPV) is a member of the Avipoxvirus (APV) and it is one of the greatest challenges facing the poultry industry, its incidence is higher in tropical and subtropical countries. It causes a significant level of morbidity and increased mortality, especially in the diphtheritic form which may reach to 50%. Avipoxvirus has been recorded in Egypt and Africa in the early of 1960, since then, it has been recorded in variable domesticated and wild bird species in different countries and Governorates. The free-living and wild birds represent a potential threat and source of infection for the domesticated poultry species. In the last ten years, the phylogenetic analysis of the partial genome sequences has gained insight into the evolutionary biology of APV in Africa. One of the main characters of APV is relative genetic stability, especially in fpv167 region of the genome. This area of the genome clustered the APV of chicken and turkey origin phylogenetically into fowl poxvirus subclade A1 together with other avipoxviruses (APVs) isolated from Galliformes worldwide. However, the pigeon poxviruses phylogenetically belong to subclade A2 with other APVs isolated from Columbiformes worldwide. The analysis of the fpv140 region provides a further comprehensive taxonomic classification based on the virus-host origin and distribution, especially in the case of the pigeon poxvirus (PGPV), which clustered separately into different subclades according to their geographical distribution. This review focus on the origin, distribution, classification and taxonomy of APVs circulating in Egypt mainly with a brief report on the situation of APVs in the other neighboring.

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Section: Virologymentioning

confidence: 99%

Section: Histopathologymentioning

confidence: 99%

Section: Histopathologymentioning

confidence: 99%

Section: Molecular Biology (Genes Clades and Subclades)mentioning

confidence: 99%

Section: Molecular Biology (Genes Clades and Subclades)mentioning

confidence: 99%

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Avipoxvirus in Egypt and African continent: A review

Lebdah

Hassanin

Ali

2019

Zagazig Veterinary Journal

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Fatal avian pox in a backyard flock of domestic ducks in Bangladesh

Pervin,

Ferdausi,

Miyuu

et al. 2023

Vet Record Case Reports

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This case study describes a fatal avian pox outbreak in a backyard flock of domestic ducks (Anas platyrhynchos domesticus) in Bangladesh. A total of six ducks of varying ages were affected and presented with solid tumour‐like masses on the legs. A 6.5‐month‐old female duck was presented for clinical examination, and a 2 cm3 solid, nodular mass was surgically excised from the left leg. Histopathological examination of the tumour mass revealed marked epidermal hyperplasia with ballooning degeneration and eosinophilic intracytoplasmic inclusion bodies, as well as severe proliferation and necrotising dermatitis, with marked heterophilic and histiocytic infiltration. Electron microscopy revealed numerous dumbbell‐shaped virus particles within the intracytoplasmic inclusion bodies on degenerating keratinocytes, confirming Avipoxvirus infection. This is the first case report of avian pox in ducks in Bangladesh.

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