Insights into grapevine defense response against drought as revealed by biochemical, physiological and RNA-Seq analysis

Haider, Muhammad Salman; Zhang, Cheng; Kurjogi, Mahantesh; Pervaiz, Tariq; Zheng, Ting; Zhang, Chaobo; Lide, Chen; Shangguan, Lingfie; Fang, Jinggui

doi:10.1038/s41598-017-13464-3

Cited by 107 publications

(84 citation statements)

References 71 publications

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“…During ROS metabolism, DEGs associated with the glutathione metabolic pathway were downregulated, such as glutathione peroxidase gene (GPX), glutathione S-transferase gene (GST), glutamate-cysteine ligase gene (GSH), and spermidine synthase gene (SPMS). Glutathione metabolism contributes to the clearance of ROS in P. ostii leaves [14], which is inconsistent with the ROS clearance system under drought stress studied by Chaves et al [40]. This may be due to drought stress exceeding ROS clearance, leading to destruction of glutathione metabolism and increased ROS content.…”

Section: Discussionmentioning

confidence: 71%

“…GSA is then converted into delta-1-pyrroline-5-carboxylate synthetase (P5CS) by spontaneous cyclization. Finally, proline is synthesized from P5C by P5C reductase (not detected) enzyme [14]. Also, proline contributes to the clearance of ROS [14].…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, the transcriptional profiles of dehydration-responsive genes in Arabidopsis, rice, and soybean were reported to be similar [13]. Thus, a transcriptomic analysis of tree peony under drought stress is of great significance, and the results can provide a basis for defense-related gene information from the global perspective [14,15].…”

Section: Introductionmentioning

confidence: 98%

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Physiological and Transcriptomic Analysis of Tree Peony (Paeonia section Moutan DC.) in Response to Drought Stress

Zhao

Zhang

Fang

et al. 2019

Forests

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Tree peony (Paeonia section Moutan DC.) is a famous ornamental plant, and P. ostii has been used for seed oil production in China because it is rich in α-linolenic acid. P. ostii has some resistance to drought, but lack of water can severely hinder its growth and development in arid areas. In order to clarify drought stress induced physiological and molecular changes of P. ostia, its physiological and transcriptomic analyses were performed under drought stress, and we found that P. ostii leaves drooped significantly 12 days after treatment and observed a significant increase in all detected physiological indices in response to drought treatment except leaf water content, chlorophyll, and carotenoid content. Meanwhile, the activity of three antioxidant enzymes basically increased under drought treatment. Moreover, drought treatment significantly reduced photosynthetic and chlorophyll fluorescence parameters except non-photochemical quenching (qN), and maintained more intact mesophyll cell structures. Additionally, many differentially expressed genes (DEGs) were found by transcriptome sequencing, which play an important role in P. ostia drought tolerance by controlling a variety of biological processes, including the reactive oxygen species (ROS) system, chlorophyll degradation and photosynthetic competency, fatty acid metabolism, proline metabolism, biosynthesis of secondary metabolism, and plant hormone metabolism. These results provide a better understanding of P. ostii responses to drought stress.

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Section: Discussionmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 99%

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Physiological and Transcriptomic Analysis of Tree Peony (Paeonia section Moutan DC.) in Response to Drought Stress

Zhao

Zhang

Fang

et al. 2019

Forests

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“…The HSP20 family is known for a strong upregulation in response to abiotic stresses (Swindell et al 2007), so the high expression levels of HSP20 found by Liu et al (2012) in leaves of 'Cabernet Sauvignon' and of HSP17.9 found by Carvalho et al (2014) in leaves of 'Touriga Nacional' upon HS were in line with findings from other species. Also, drought stress causes HSP upregulation, as found by Haider et al (2017;Table 1) in leaves of 'Summer Black'. The authors also reported that upregulated DEGs were about twofold as compared to those that were downregulated, modulating defense-related pathways such as ROS defense system but also basic metabolism such as chlorophyll synthesis.…”

Section: Transcriptomic Studies In Stress Response: the Relevance Of mentioning

confidence: 75%

Cutting the Gordian Knot of abiotic stress in grapevine: From the test tube to climate change adaptation

Carvalho

Amâncio

2018

Physiologia Plantarum

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In Mediterranean climate areas, the available scenarios for climate change suggest an increase in the frequency of heat waves and severe drought in summer. Grapevine (Vitis vinifera L.) is a traditional Mediterranean species and is the most valuable fruit crop in the world. Currently, viticulture must adjust to impending climate changes that are already pushing vine‐growers toward the use of irrigation, with the concomitant losses in wine quality, and researchers to study tolerance to stress in existing genotypes. The viticulture and winemaking worlds are in demand to understand the physiological potential of the available genotypes to respond to climate changes. In this review, we will focus on the cross‐talk between common abiotic stresses that currently affect grapevine productivity and that are prone to affect it deeper in the future. We will discuss results obtained under three experimental stress conditions and that call for specific responses: (1) acclimatization of in vitro plantlets, (2) stress combinations in controlled conditions for research purposes, (3) extreme events in the field that, driven by climate changes, are pushing Mediterranean species to the limit. The different levels of tolerance to stress put in evidence by the plasticity of phenotypic and genotypic response mechanisms, will be addressed. This information is relevant to understand varietal adaptation to impending climate changes and to assist vine growers in choosing genotypes and viticulture practices.

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“…Abiotic stresses considerably harbor plant growth and productivity, thus lead to economic losses. The drought severity hampered grapevine productivity and has deleterious impacts on viticulture worldwide [41,42]. Salt stress is one of the severe abiotic stress, while grapevine plants generally modify their physiology to combat salt stress severity [43].…”

Section: Discussionmentioning

confidence: 99%

Genome-wide Identification of the POD Gene Family and their Expression Profiling in Grapevine (Vitis vinifera L)

Xiao

Wang

Khan

et al. 2019

Preprint

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Abstract The purpose of this study is by extending the analysis of class III peroxidases (PODs) in grapevine and provide further insights into the organ-specific developmental role in transcriptional dynamics and gene duplication analysis of this economically important fruit crop species. Herein, we comprehensively identified 47 PODs in the grapevine genome and are further classified into 7 subgroups based on their phylogenetic analysis. Results of motif composition and gene structure organization analysis revealed that PODs in the same subgroup shared similar conjunction while the protein sequences were highly conserved. Intriguingly, the integrated analysis of chromosomal mapping and gene collinearity analysis proposed that both dispersed and tandem duplication events contributed to the expansion of PODs in grapevine. Also, the gene duplication analysis suggested that most of the genes (20) were dispersed followed by (15) tandem, (9) segmental or whole-genome duplication, and (3) proximal, respectively. The evolutionary analysis of PODs, such as Ka/Ks ratio of the 15 duplicated gene pairs were less than 1.00, indicated that most of the gene pairs exhibiting purifying selection and 7 pairs underwent positive selection with value greater than 1.00. The Gene Ontology Enrichment (GO), Kyoto Encyclopedia of Genes Genomics (KEGG) analysis, and cis-elements prediction also revealed the positive functions of PODs in plant growth and developmental activities, and response to stress stimuli. Further, based on the publically available RNA-sequence data, the expression patterns of PODs in tissue-specific response during several developmental stages revealed diverged expression patterns. Subsequently, 30 genes were selected for RT-PCR validation in response to (NaCl, drought, and ABA), which showed their critical role in grapevine. In conclusion, we predict that these results will lead to novel insights regarding genetic improvement of grapevine.

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Insights into grapevine defense response against drought as revealed by biochemical, physiological and RNA-Seq analysis

Cited by 107 publications

References 71 publications

Physiological and Transcriptomic Analysis of Tree Peony (Paeonia section Moutan DC.) in Response to Drought Stress

Physiological and Transcriptomic Analysis of Tree Peony (Paeonia section Moutan DC.) in Response to Drought Stress

Cutting the Gordian Knot of abiotic stress in grapevine: From the test tube to climate change adaptation

Genome-wide Identification of the POD Gene Family and their Expression Profiling in Grapevine (Vitis vinifera L)

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