Insights into Detection and Identification of Foodborne Pathogens

Law, Jodi Woan‐Fei; Letchumanan, Vengadesh; Chan, Kok‐Gan; Goh, Bey Hing; Lee, Learn‐Han

doi:10.1002/9781119139188.ch7

Cited by 11 publications

(11 citation statements)

References 317 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Polymerase chain reaction (PCR) was used to detect the highly conserved species-specific gene toxR and the virulence genes tdh and trh in all V. parahaemolyticus isolates (Law et al, 2017). Genomic DNA was extracted using a bacterial DNA extraction kit (Sangon, Shanghai, China) according to the manufacturer's instructions.…”

Section: Molecular Identification and Virulence Gene Detection Of V mentioning

confidence: 99%

Co-occurrence of Antibiotic and Heavy Metal Resistance and Sequence Type Diversity of Vibrio parahaemolyticus Isolated From Penaeus vannamei at Freshwater Farms, Seawater Farms, and Markets in Zhejiang Province, China

Jiang

Yang

et al. 2020

Front. Microbiol.

View full text Add to dashboard Cite

Vibrio parahaemolyticus is the leading cause of seafood-borne bacterial poisoning in China and is a threat to human health worldwide. The aim of this study was to assess the antibiotic resistance profiles and distribution of heavy metal resistance of V. parahaemolyticus isolates from Penaeus vannamei from freshwater farms, seawater farms, and their corresponding markets in Zhejiang, China and to assess the relationship between multidrug resistance (MDR) and multi-heavy metal resistance (MHMR). Of the 360 P. vannamei samples that we tested, 90 (25.00%) were V. parahaemolyticus positive, but the occurrence of pathogenic isolates carrying the toxin genes tdh (4.44%) and trh (3.33%) was low. None of the tested isolates harbored both the tdh and trh genes. However, antibiotic resistance profiles varied among different sampling locations, levels of resistance to the antibiotics ampicillin (76.67%) and streptomycin (74.44%) were high overall, and MDR isolates were common (40.00% of all isolates). Heavy metal resistance patterns were similar among the different sampling locations. Overall, the majority of V. parahaemolyticus isolates displayed tolerance to Cd 2+ (60.00%), and fewer were resistant to Cu 2+ (40.00%), Zn 2+ (38.89%), Ni 2+ (24.44%), Cr 3+ (14.44%), and Co 2+ (8.89%). In addition, 34.44% (31/90) of isolates tested in this study were found to be MHMR. Using Pearson's correlation analysis, MDR and MHMR were found to be positively correlated (P = 0.004; R = 0.759). The 18 V. parahaemolyticus isolates that were both MDR and MHMR represented 18 sequence types, of which 12 were novel to the PubMLST database, and displayed a high level of genetic diversity, suggesting that dissemination may be affected by mobile genetic elements via horizontal gene transfer. However, a low percentage of class 1 integrons without gene cassettes and no class 2 or 3 integrons were detected in the 18 MDR and MHMR isolates or in the

show abstract

Section: Molecular Identification and Virulence Gene Detection Of V mentioning

confidence: 99%

Co-occurrence of Antibiotic and Heavy Metal Resistance and Sequence Type Diversity of Vibrio parahaemolyticus Isolated From Penaeus vannamei at Freshwater Farms, Seawater Farms, and Markets in Zhejiang Province, China

Jiang

Yang

et al. 2020

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…[5,83,84] , Vibrio sp. [85][86][87][88][89][90][91] , and Salmonella sp. [92][93][94][95][96] that commonly affect human and animal health.…”

Section: Resultsmentioning

confidence: 99%

Decoding the mystery of how bacteria “talk”: Among Gram-negative microorganisms

Tan¹,

Law²,

Letchumanan³

et al. 2019

Prog Micobes Mol Biol

Self Cite

View full text Add to dashboard Cite

To date, microbial diversity is still the least well understood component of biodiversity. Bacteria are the most abundant microorganisms where most species are often found ubiquitous. Microorganisms such as bacteria are diverse in their impacts such as in spreading of infectious diseases or play a valuable role in biotechnological purposes. Hence, it is interesting to gain a look upon the ways where bacteria regulate their daily processes in the environment. Bacteria communicate with each other through extracellular signalling molecules or also known as autoinducers (AIs) that are produced, detected and show response. This process is termed as quorum sensing (QS) which indicates that bacteria do communicate in order to perform various physiological activities. QS enable bacteria to have the advantages that are unattainable as individual bacterial cell. This review emphases on the characteristics of quorum sensing (QS) and its benefits in understanding different kind of bacterial QS-dependent activities. This fundamental insight from QS system will enable us to manage bacterial activities by targeting their communication circuit.

show abstract

“…Vibriosis becomes a huge public health concern as it may cause severe illness in human and animal with detrimental consequences (Law et al . 2016). As aquaculture is intensive, the animals are cultivated in high densities to maintain continuous production and meet consumer’s demand.…”

Section: Introductionmentioning

confidence: 99%

Incidence of antibiotic resistance in Vibrio spp

Loo

Letchumanan

Law

et al. 2020

Reviews in Aquaculture

Self Cite

View full text Add to dashboard Cite

Vibrio species is a common natural inhabitant of brackish and saltwater. Vibrio sp. includes not only emerging pathogens but also well‐known and emerged pathogens, such as V. cholerae, V. parahaemolyticus and V. vulnificus. Although it is often harmless, pathogenic strains which could cause serious food‐borne illnesses/outbreaks in both humans and animals have been identified. Vibrio infection is usually self‐limiting and requires no clinical treatment. However, antibiotics are still required to treat severe cases of Vibrio infection. Recent reports have highlighted a rise in the presence of antibiotic‐resistant Vibrio in the environment. This hampers the clinical treatment of Vibrio infection and has been linked to the imprudent use of antibiotics in various fields such as human medicine, agriculture and animal husbandry. As a result, it has greatly jeopardized food security, threatened public health and impugned the growth of economy. Food is a potential vehicle of antibiotic‐resistant Vibrio where the resistant genes are transmitted to humans via ingestion of contaminated food. Hence, the surveillance, monitoring and management of antibiotic patterns of Vibrio are important for clinical treatment. This review focuses on different trends and global patterns of antibiotic resistance of Vibrio.

show abstract

Insights into Detection and Identification of Foodborne Pathogens

Cited by 11 publications

References 317 publications

Co-occurrence of Antibiotic and Heavy Metal Resistance and Sequence Type Diversity of Vibrio parahaemolyticus Isolated From Penaeus vannamei at Freshwater Farms, Seawater Farms, and Markets in Zhejiang Province, China

Co-occurrence of Antibiotic and Heavy Metal Resistance and Sequence Type Diversity of Vibrio parahaemolyticus Isolated From Penaeus vannamei at Freshwater Farms, Seawater Farms, and Markets in Zhejiang Province, China

Decoding the mystery of how bacteria “talk”: Among Gram-negative microorganisms

Incidence of antibiotic resistance in Vibrio spp

Contact Info

Product

Resources

About