Insights into Characterization Methods and Biomedical Applications of Nanoparticle–Protein Corona

Li, Yan; Lee, Jaeseung

doi:10.3390/ma13143093

Cited by 36 publications

(43 citation statements)

References 185 publications

(261 reference statements)

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“…Each biological microenvironment has a distinct set of proteins that interact in a specific way with the particles’ surface. Protein adsorption to particles does not always require direct contact with the particle surface and may instead occur via protein-protein interactions ( Li and Lee, 2020 ). In addition, proteins can undergo conformational changes that together with nonspecific protein-protein interactions may be interpreted as a danger-associated signal ( Corbo et al, 2016 ); initiating an immune response that may develop into a successful defensive response or an uncalibrated inflammatory reaction ( Boraschi et al, 2020 ).…”

Section: Impact Of Particles’ Properties and Acquired Biological Iden...mentioning

confidence: 99%

Particle Safety Assessment in Additive Manufacturing: From Exposure Risks to Advanced Toxicology Testing

et al. 2022

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Additive manufacturing (AM) or industrial three-dimensional (3D) printing drives a new spectrum of design and production possibilities; pushing the boundaries both in the application by production of sophisticated products as well as the development of next-generation materials. AM technologies apply a diversity of feedstocks, including plastic, metallic, and ceramic particle powders with distinct size, shape, and surface chemistry. In addition, powders are often reused, which may change the particles’ physicochemical properties and by that alter their toxic potential. The AM production technology commonly relies on a laser or electron beam to selectively melt or sinter particle powders. Large energy input on feedstock powders generates several byproducts, including varying amounts of virgin microparticles, nanoparticles, spatter, and volatile chemicals that are emitted in the working environment; throughout the production and processing phases. The micro and nanoscale size may enable particles to interact with and to cross biological barriers, which could, in turn, give rise to unexpected adverse outcomes, including inflammation, oxidative stress, activation of signaling pathways, genotoxicity, and carcinogenicity. Another important aspect of AM-associated risks is emission/leakage of mono- and oligomers due to polymer breakdown and high temperature transformation of chemicals from polymeric particles, both during production, use, and in vivo, including in target cells. These chemicals are potential inducers of direct toxicity, genotoxicity, and endocrine disruption. Nevertheless, understanding whether AM particle powders and their byproducts may exert adverse effects in humans is largely lacking and urges comprehensive safety assessment across the entire AM lifecycle—spanning from virgin and reused to airborne particles. Therefore, this review will detail: 1) brief overview of the AM feedstock powders, impact of reuse on particle physicochemical properties, main exposure pathways and protective measures in AM industry, 2) role of particle biological identity and key toxicological endpoints in the particle safety assessment, and 3) next-generation toxicology approaches in nanosafety for safety assessment in AM. Altogether, the proposed testing approach will enable a deeper understanding of existing and emerging particle and chemical safety challenges and provide a strategy for the development of cutting-edge methodologies for hazard identification and risk assessment in the AM industry.

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Section: Impact Of Particles’ Properties and Acquired Biological Iden...mentioning

confidence: 99%

Particle Safety Assessment in Additive Manufacturing: From Exposure Risks to Advanced Toxicology Testing

et al. 2022

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“…Protein–gold nanoparticle conjugates are poised to play a critical role in next-generation biomedical technologies. − Advances in diagnostic testing, − tissue and cellular imaging, − photothermal therapy, biocatalysis, and drug delivery have been achieved with the integration of functionalized gold nanoparticles. The overall success and widespread implementation of these nanoparticle-enabled technologies hinges on the effectiveness of the protein–nanoparticle conjugate, specifically the interfacial chemistry to immobilize the surface protein.…”

Section: Introductionmentioning

confidence: 99%

High-Affinity Points of Interaction on Antibody Allow Synthesis of Stable and Highly Functional Antibody–Gold Nanoparticle Conjugates

et al. 2021

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Many emerging nanobiotechnologies rely on the proper function of proteins immobilized on gold nanoparticles. Often, the surface chemistry of the AuNP is engineered to control the orientation, surface coverage, and structure of the adsorbed protein to maximize conjugate function. Here, we chemically modified antibody to investigate the effect of protein surface chemistries on adsorption to AuNPs. A monoclonal anti-horseradish peroxidase IgG antibody (anti-HRP) was reacted with N-succinimidyl acrylate (NSA) or reduced dithiobissuccinimidyl propionate (DSP) to modify lysine residues. Zeta potential measurements confirmed that both chemical modifications reduced the localized regions of positive charge on the protein surface, while the DSP modification incorporated additional free thiols. Dynamic light scattering confirmed that native and chemically modified antibodies adsorbed onto AuNPs to form bioconjugates; however, adsorption kinetics revealed that the NSA-modified antibody required significantly more time to allow for the formation of a hard corona. Moreover, conjugates formed with the NSA-modified antibody lost antigen-binding function, whereas unmodified and DSP-modified antibodies adsorbed onto AuNPs to form functional conjugates. These results indicate that high-affinity functional groups are required to prevent protein unfolding and loss of function when adsorbed on the AuNP surface. The reduced protein charge and high-affinity thiol groups on the DSP-modified antibody enabled pH-dependent control of protein orientation and the formation of highly active conjugates at solution pHs (<7.5) that are inaccessible with unmodified antibody due to conjugate aggregation. This study establishes parameters for protein modification to facilitate the formation of highly functional and stable protein–AuNP conjugates.

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“…The intravenous delivery of these NPs have a variety of challenges associated therein. One such challenge is a change in the characteristics of the original MNPs, due to the formation of protein corona [81] [82] . Another challenge is the presence of phagocytic immune cells and their ability to recognize the NPs in question, which in turn can hinder their movement or induce an immune response.…”

Section: Synthesis Methods Suitable For In-vivo Applicationsmentioning

confidence: 99%

Designing magnetic nanoparticles for in vivo applications and understanding their fate inside human body

Senthilkumar

Sharma

Sood

et al. 2021

Coordination Chemistry Reviews

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Insights into Characterization Methods and Biomedical Applications of Nanoparticle–Protein Corona

Cited by 36 publications

References 185 publications

Particle Safety Assessment in Additive Manufacturing: From Exposure Risks to Advanced Toxicology Testing

Particle Safety Assessment in Additive Manufacturing: From Exposure Risks to Advanced Toxicology Testing

High-Affinity Points of Interaction on Antibody Allow Synthesis of Stable and Highly Functional Antibody–Gold Nanoparticle Conjugates

Designing magnetic nanoparticles for in vivo applications and understanding their fate inside human body

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