Insight on stem cell preconditioning and instructive biomaterials to enhance cell adhesion, retention, and engraftment for tissue repair

Shafiq, Muhammad; Jung, Youngmee; Kim, Soo Hyun

doi:10.1016/j.biomaterials.2016.03.020

Cited by 103 publications

(89 citation statements)

References 303 publications

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“…4,5 This unexpected, suboptimal therapeutic outcome might be associated with the poor survival, retention, and engraftment of transplanted stem cells. [6][7][8] Therefore, knowledge of the long-term fate of stem cells including survival, biodistribution, migration, and differentiation after transplantation is essential for improving functional benefits of MSC-based stroke treatment. Monitoring of the fate of therapeutic cells is typically achieved by postmortem histological analysis at predetermined time points after cell transplantation -which is laborious and invasive and,…”

Section: Introductionmentioning

confidence: 99%

“…Possible reasons might include hypoxia, serum deprivation-induced apoptosis, necrosis, mechanical damage, immunological rejection, and infiltration of inflammatory microglia. 7,8,32 Among them, hypoxia-and/or serum deprivation-induced apoptosis and immunological rejection are the major factors. 30,32 In our study, the total number of apoptotic GFP-MSCs peaked at 1 week, and then gradually decreased over time.…”

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confidence: 99%

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The long-term fate of mesenchymal stem cells labeled with magnetic resonance imaging-visible polymersomes in cerebral ischemia

Duan

Wang

et al. 2017

IJN

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Understanding the long-term fate and potential mechanisms of mesenchymal stem cells (MSCs) after transplantation is essential for improving functional benefits of stem cell-based stroke treatment. Magnetic resonance imaging (MRI) is considered an attractive and clinically translatable tool for longitudinal tracking of stem cells, but certain controversies have arisen in this regard. In this study, we used SPION-loaded cationic polymersomes to label green fluorescent protein (GFP)-expressing MSCs to determine whether MRI can accurately reflect survival, long-term fate, and potential mechanisms of MSCs in ischemic stroke therapy. Our results showed that MSCs could improve the functional outcome and reduce the infarct volume of stroke in the brain. In vivo MRI can verify the biodistribution and migration of grafted cells when pre-labeled with SPION-loaded polymersome. The dynamic change of low signal volume on MRI can reflect the tendency of cell survival and apoptosis, but may overestimate long-term survival owing to the presence of iron-laden macrophages around cell graft. Only a small fraction of grafted cells survived up to 8 weeks after transplantation. A minority of these surviving cells were differentiated into astrocytes, but not into neurons. MSCs might exert their therapeutic effect via secreting paracrine factors rather than directing cell replacement through differentiation into neuronal and/or glial phenotypes.

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Section: Introductionmentioning

confidence: 99%

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confidence: 99%

The long-term fate of mesenchymal stem cells labeled with magnetic resonance imaging-visible polymersomes in cerebral ischemia

Duan

Wang

et al. 2017

IJN

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“…Furthermore, the prompt in vivo induction of angiogenesis is often mandatory (e.g. myocardium) [5], whereas other tissues retain their function upon implantation only if their avascular nature is preserved in healthy condition (e.g. hyaline cartilage, cardiac valve substitute) [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

“…To induce angiogenesis, the delivery of relevant pro-angiogenic factors [5,11] as well as of progenitor or mature endothelial cells is often used [5]. Mesenchymal stromal cells have been largely used in many clinical applications for regenerative/repair purposes, thanks to their capability to release a broad range of factors (e.g.…”

Section: Introductionmentioning

confidence: 99%

Control of angiogenesis and host response by modulating the cell adhesion properties of an Elastin-Like Recombinamer-based hydrogel

et al. 2017

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The control of the in vivo vascularization of engineered tissue substitutes is essential in order to obtain either a rapid induction or a complete inhibition of the process (e.g. in muscles and hyaline-cartilage, respectively). Among the several polymers available, Elastin-Like Recombinamers (ELRs)-based hydrogel stands out as a promising material for tissue engineering thanks to its viscoelastic properties, non-toxicity, and non-immunogenicity. In this study, we aimed to modulate the high angiogenic potential of adipose tissue-derived stromal vascular fraction (SVF) cells, predominantly composed of endothelial/mural and mesenchymal cells, by simply varying the cell adhesion properties of ELRs-hydrogels. Human SVF cells, embedded in RGD-REDVbioactivated or unmodified ELRs-hydrogels, were implanted in rat subcutaneous pockets either immediately or upon 5-day-culture in perfusion-bioreactors. Perfusionbased culture enhanced the endothelial cell cord-like-organization and the release of pro-angiogenic factors in functionalized constructs. While in vivo vascularization and host cell infiltration within the bioactivated gels were highly enhanced, the two processes were strongly inhibited in non-functionalized hydrogels up to 28 days. ELRsbased hydrogels showed a great potential to determine the successful integration of engineered substitutes thanks to their capacity to finely control the angiogenic/inflammation process at the recipient site, even in presence of SVF cells.

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“…Hydroxyapatite, calcium phosphate, and composites based on them, which have a developed porous structure and are good bone substituents are often used. However, these materials are low -tech and brittle 2 .…”

Section: Introductionmentioning

confidence: 99%

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2018

IJPSR

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Bulk nanocomposites prepared from aqueous albumin dispersion with carbon nanotubes by removing the liquid component from the dispersion have been investigated. The composites were obtained by thermostating and exposure to LED and IR diode laser radiation. The nanocomposites obtained under laser irradiation retain their shape and properties for several years, in contrast to the composites fabricated in different ways (which decompose into small fragments immediately after preparation). The low density of the composites under study (~1200 kg/m 3 ), which is close to the density of water, is due to their high porosity. The hardness of stable nanocomposites (~300 MPa) was found to be at the same level as the hardness of polymethylmethacrylate, aluminium, and iron and close to the hardness of human bone tissue. The cluster quasi ordering of the inner structure of nanocomposites revealed by atomic force microscopy indicates the possibility of forming a bulk nanotube framework in them, which can be caused by the effect of the electric field of laser radiation and ensure their stability and hardness. The presence of a framework in nanocomposites provides conditions for self-assembly of biological tissues and makes it possible to apply laser-prepared nanocomposites as a component of surgical implants.

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Insight on stem cell preconditioning and instructive biomaterials to enhance cell adhesion, retention, and engraftment for tissue repair

Cited by 103 publications

References 303 publications

The long-term fate of mesenchymal stem cells labeled with magnetic resonance imaging-visible polymersomes in cerebral ischemia

The long-term fate of mesenchymal stem cells labeled with magnetic resonance imaging-visible polymersomes in cerebral ischemia

Control of angiogenesis and host response by modulating the cell adhesion properties of an Elastin-Like Recombinamer-based hydrogel

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