Insight into the microbial interactions of Anammox and heterotrophic bacteria in different granular sludge systems: effect of size distribution and available organic carbon source

Fan, Jiarui; Du, Rui; Liu, Qingtao; Liu, Cong; Peng, Yongzhen

doi:10.1016/j.biortech.2022.128055

Cited by 14 publications

(5 citation statements)

References 44 publications

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“…HNU0055) related genes ( gnd and gltA ) were particularly active. It was probably due to the symbiotic relationship between anammox bacteria and heterotrophic bacteria …”

Section: Resultsmentioning

confidence: 99%

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Mixotrophic Metabolism and Oxygen Detoxification Mitigate Antibiotic Stress in Mainstream Anammox Process Under Microaerobic Condition

Wang,

Han,

Shi

et al. 2024

ACS EST Water

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Given the prevalence and risk of antibiotics and advantages of the anaerobic ammonium oxidation (anammox) process, this study evaluated and compared the mainstream and sidestream anammox process performance in treating wastewater containing typical β-lactam antibiotic under microaerobic conditions. The mainstream anammox process was able to tolerate higher concentrations of cefalexin under the relatively low dissolved oxygen (DO) concentration, while higher DO slightly reduced the nitrogen removal efficiency of the anammox process, but it was still higher than 70%. In contrast, the sidestream anammox process exhibited a poor tolerance to cefalexin stress. Combined with metagenomics and transcriptomics, the comparative analysis of 154 high-quality metagenomic assembly genomes in anammox consortia revealed the potential mechanism of low DO in mitigating cefalexin stress. Results showed that the expressions of genes related to the dissimilatory nitrate reduction to ammonia process and anammox process were significantly upregulated under low DO condition. As the dominant functional bacteria, Candidatus Kuenenia stuttgartiensis HNU0043 had the detoxification mechanism of •O 2 − metabolism and secondary product H 2 O 2 , which mainly depended on the cooperation of Fe−Mn family superoxide dismutase and catalase. Expressions of genes involved in various carbon metabolism pathways were also detected in Ca. Kuenenia stuttgartiensis HNU0043, explaining the resistance of anammox consortia to antibiotic stress through biodegradation. Therefore, the mainstream anammox process tolerated the antibiotic stress under microaerobic conditions, while low DO was more beneficial for maintaining its stability. This work provides insights into the molecular mechanisms underlying anammox microbiota adaptability to antibiotics and oxygen, further expending the implementation of the anammox process.

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“…HNU0055) related genes ( gnd and gltA ) were particularly active. It was probably due to the symbiotic relationship between anammox bacteria and heterotrophic bacteria …”

Section: Resultsmentioning

confidence: 99%

“…It was probably due to the symbiotic relationship between anammox bacteria and heterotrophic bacteria. 29 A total of 281 ARGs were identified and distributed in 95 MAGs and 13 antibiotic categories. Among them, 25 MAGs contained a single resistance gene, while 70 MAGs exhibited multiple resistances.…”

Section: Resultsmentioning

confidence: 99%

Mixotrophic Metabolism and Oxygen Detoxification Mitigate Antibiotic Stress in Mainstream Anammox Process Under Microaerobic Condition

Wang,

Han,

Shi

et al. 2024

ACS EST Water

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“…However, anammox, as a chemolithoautotrophic process, also exhibited a significant and positive correlation with TC, DOC and SOC contents (Figure 3a). Previous study has demonstrated that organic C addition could benefit the augment of anammox potential by enhancing the utilization potential of anammox for CO 2 (Zhang, Yin, Shi, & Wu, 2021), especially the addition of those simple available organic C (Fan et al., 2022). In this study, the native soil pH for the CK treatment was 7.98.…”

Section: Discussionmentioning

confidence: 99%

Long‐term fertilization regulates dissimilatory nitrate reduction processes by altering paddy soil organic carbon components

Li,

Wei,

Wang

et al. 2023

Soil Use and Management

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To enhance rice yields, synthetic nitrogen (N) fertilizers are often applied in rice paddy fields. Under waterlogged conditions, denitrification, anaerobic ammonium oxidation (anammox) and dissimilatory nitrate reduction to ammonium (DNRA) are the main N transformation processes. However, the effects of long‐term fertilization on the dissimilatory nitrate reduction processes remain largely unclear. Here, we investigated rates of denitrification, anammox and DNRA and the abundance of bacterial and nitrate reduction‐related genes (16S rRNA, narG, nirK, nirS, nosZ and hzsB) in rice paddy fields under different fertilization regimes [i.e., non‐fertilization (CK), NPK fertilization (NPK) and NPK fertilization plus straw returning (NPKS)]. The rates of denitrification, anammox and DNRA in the CK treatment were 46.18, 0.35 and 1.29 nmoL N g−1 h−1, respectively, which were significantly (p < .05) increased by 7.32%–27.78%, 2.45–4.12 folds and 1.01–13.23 folds by NPK and NPKS treatments. This was largely consistent with the increased bacterial and nitrate reduction‐related gene abundances in the NPK and NPKS treatments. In addition, NPKS also significantly increased the relative contribution of DNRA to the total nitrate reduction relative to NPK and CK treatments, indicating that NPKS was more beneficial to N retention in the paddy field. Changes in rates of dissimilatory nitrate reduction processes in response to NPK and NPKS treatments were correlated to variations of soil total N, ammonium contents, pH, soil organic carbon (SOC) contents and nitrate reduction‐related genes, among which SOC contents had the highest explanation. Specifically, fulvic‐like compounds, alkyl C and methoxyl and nitrogen‐alkyl C were the key components determining denitrification, anammox and DNRA rates, respectively. Overall, our study suggests that long‐term NPK and NPKS fertilization stimulate rates of dissimilatory nitrate reduction processes mainly by altering soil C components and NPKS is more effective in maintaining soil fertility in the paddy field.

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“…These results provide evidence for the positive contributions of DNRA towards robust reactor performance. The interactions between anammox and DNRA bacteria can be enhanced by organic carbon amendments to encourage the growth of DNRA microbes, which has been previously reported to have beneficial effects on reactor performance ( Jenni et al, 2014 ; Zhang et al, 2021 ; Fan et al, 2022 ). Taking advantage of the synergy between anammox and DNRA could be especially advantageous during reactor startup, where anammox activity is vulnerable to performance disruptions due to nitrite inhibition.…”

Section: Discussionmentioning

confidence: 99%

Synergistic interactions between anammox and dissimilatory nitrate reducing bacteria sustains reactor performance across variable nitrogen loading ratios

et al. 2023

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Anaerobic ammonium oxidizing (anammox) bacteria are utilized for high efficiency nitrogen removal from nitrogen-laden sidestreams in wastewater treatment plants. The anammox bacteria form a variety of competitive and mutualistic interactions with heterotrophic bacteria that often employ denitrification or dissimilatory nitrate reduction to ammonium (DNRA) for energy generation. These interactions can be heavily influenced by the influent ratio of ammonium to nitrite, NH4+:NO2−, where deviations from the widely acknowledged stoichiometric ratio (1:1.32) have been demonstrated to have deleterious effects on anammox efficiency. Thus, it is important to understand how variable NH4+:NO2− ratios impact the microbial ecology of anammox reactors. We observed the response of the microbial community in a lab scale anammox membrane bioreactor (MBR) to changes in the influent NH4+:NO2− ratio using both 16S rRNA gene and shotgun metagenomic sequencing. Ammonium removal efficiency decreased from 99.77 ± 0.04% when the ratio was 1:1.32 (prior to day 89) to 90.85 ± 0.29% when the ratio was decreased to 1:1.1 (day 89–202) and 90.14 ± 0.09% when the ratio was changed to 1:1.13 (day 169–200). Over this same timespan, the overall nitrogen removal efficiency (NRE) remained relatively unchanged (85.26 ± 0.01% from day 0–89, compared to 85.49 ± 0.01% from day 89–169, and 83.04 ± 0.01% from day 169–200). When the ratio was slightly increased to 1:1.17–1:1.2 (day 202–253), the ammonium removal efficiency increased to 97.28 ± 0.45% and the NRE increased to 88.21 ± 0.01%. Analysis of 16 S rRNA gene sequences demonstrated increased relative abundance of taxa belonging to Bacteroidetes, Chloroflexi, and Ignavibacteriae over the course of the experiment. The relative abundance of Planctomycetes, the phylum to which anammox bacteria belong, decreased from 77.19% at the beginning of the experiment to 12.24% by the end of the experiment. Analysis of metagenome assembled genomes (MAGs) indicated increased abundance of bacteria with nrfAH genes used for DNRA after the introduction of lower influent NH4+:NO2− ratios. The high relative abundance of DNRA bacteria coinciding with sustained bioreactor performance indicates a mutualistic relationship between the anammox and DNRA bacteria. Understanding these interactions could support more robust bioreactor operation at variable nitrogen loading ratios.

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Insight into the microbial interactions of Anammox and heterotrophic bacteria in different granular sludge systems: effect of size distribution and available organic carbon source

Cited by 14 publications

References 44 publications

Mixotrophic Metabolism and Oxygen Detoxification Mitigate Antibiotic Stress in Mainstream Anammox Process Under Microaerobic Condition

Mixotrophic Metabolism and Oxygen Detoxification Mitigate Antibiotic Stress in Mainstream Anammox Process Under Microaerobic Condition

Long‐term fertilization regulates dissimilatory nitrate reduction processes by altering paddy soil organic carbon components

Synergistic interactions between anammox and dissimilatory nitrate reducing bacteria sustains reactor performance across variable nitrogen loading ratios

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