Insight into the interactions between nanoparticles and cells

Li, Jingchao; Mao, Hongli; Naoki, Katsuhiko; Chen, Guoping

doi:10.1039/c6bm00714g

Cited by 84 publications

(53 citation statements)

References 224 publications

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“…36 This would also explain the increased vitality observed in HUVECs treated with MSNPs of 30 nm size. With in vitro experiments especially, it would be appropriate to consider the growth conditions of different cellular models.…”

Section: Analysis Of Msnp Protein Coronas By Sds-pagementioning

confidence: 85%

Mesoporous silica nanoparticles trigger mitophagy in endothelial cells and perturb neuronal network activity in a size- and time-dependent manner

Orlando¹,

Cazzaniga²,

Tringali³

et al. 2017

IJN

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Purpose Mesoporous silica nanoparticles (MSNPs) are excellent candidates for biomedical applications and drug delivery to different human body areas, the brain included. Although toxicity at cellular level has been investigated, we are still far from using MSNPs in the clinic, because the mechanisms involved in the cellular responses activated by MSNPs have not yet been elucidated. Materials and methods This study used an in vitro multiparametric approach to clarify relationships among size, dose, and time of exposure of MSNPs (0.05–1 mg/mL dose range), and cellular responses by analyzing the morphology, viability, and functionality of human vascular endothelial cells and neurons. Results The results showed that 24 hours of exposure of endothelial cells to 250 nm MSNPs exerted higher toxicity in terms of mitochondrial activity and membrane integrity than 30 nm MSN at the same dose. This was due to induced cell autophagy (in particular mitophagy), probably consequent to MSNP cellular uptake (>20%). Interestingly, after 24 hours of treatment with 30 nm MSNPs, very low MSNP uptake (<1%) and an increase in nitric oxide production (30%, P <0.01) were measured. This suggests that MSNPs were able to affect endothelial functionality from outside the cells. These differences could be attributed to the different protein-corona composition of the MSNPs used, as suggested by sodium dodecyl sulfate polyacrylamide-gel electrophoresis analysis of the plasma proteins covering the MSNP surface. Moreover, doses of MSNPs up to 0.25 mg/mL perturbed network activity by increasing excitability, as detected by multielectrode-array technology, without affecting neuronal cell viability. Conclusion These results suggest that MSNPs may be low-risk if prepared with a diameter <30 nm and if they reach human tissues at doses <0.25 mg/mL. These important advances could help the rational design of NPs intended for biomedical uses, demonstrating that careful toxicity evaluation is necessary before using MSNPs in patients.

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Section: Analysis Of Msnp Protein Coronas By Sds-pagementioning

confidence: 85%

Mesoporous silica nanoparticles trigger mitophagy in endothelial cells and perturb neuronal network activity in a size- and time-dependent manner

Orlando¹,

Cazzaniga²,

Tringali³

et al. 2017

IJN

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show abstract

“…(9-11) Accordingly, there have been efforts to target viruses to cancer sites, in a manner analogous to those adopted for targeting synthetic nanoparticles. (12)(13)(14)(15) The conjugation of synthetic polymers to provide 'stealth' coatings for oncolytic viruses is one means by which extended circulation and tumour targeting might be achieved. A number of papers have reported successful conjugation of polymers to synthetic viruses, (16)(17)(18) with concomitant changes in viral biodistribution and targeting.…”

Section: Introductionmentioning

confidence: 99%

“…3H, C=C-CH3), 1.18 (dd, J = 6.3, 1.9 Hz, 3H, CH3) 13. C NεR (100 εHz, CDCl3)μ = 169.55 (1C, C=O), 139.62 (1C, C=CH2), 120.13 (1C, C=CH2), 67.03 (1C, CHOH), 47.19 (1C, CH2), 20.89 (1C, CHCH3), 18.66 (1C, CCH3).…”

mentioning

confidence: 99%

Polyvalent Diazonium Polymers Provide Efficient Protection of Oncolytic Adenovirus Enadenotucirev from Neutralizing Antibodies while Maintaining Biological Activity In Vitro and In Vivo

et al. 2019

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antibodies resulting in lower transgene production. However, in the presence of neutralising antibodies some in vivo transgene expression was maintained for coated virus compared to the uncoated control. The decrease in transgene expression was found not to be solely due to lower cellular uptake but due to reduced unpackaging of the virus within the cells and reduced replication indicating that polymer coating does not cause permanent inactivation of the virus.These data suggest that virus activity may be modulated by appropriate design of coating polymers while retaining protection against neutralising antibodies.

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“…For example, nanotextured surfaces and nanostructures have been used to mimic or to interact with biological environments, to support specific biological functions such as cell adhesion, mobility, and differentiation, as well as tissue healing . In this context, nanoscale structures and materials have been widely explored in many biomedical applications owing to their novel and impressive physical/chemical properties, that offer unprecedented opportunities to study and interact with complex biological processes . Recently, other groups and ours have proposed a new paradigm in nanomedicine consisting in the exploitation of the intrinsic properties of nanomaterials as active devices rather than as passive structural units or carriers for medications .…”

Section: Introductionmentioning

confidence: 99%

Remote Control of Cellular Functions: The Role of Smart Nanomaterials in the Medicine of the Future

Genchi

Marino

Grillone

et al. 2017

Adv Healthcare Materials

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The remote control of cellular functions through smart nanomaterials represents a biomanipulation approach with unprecedented potential applications in many fields of medicine, ranging from cancer therapy to tissue engineering. By actively responding to external stimuli, smart nanomaterials act as real nanotransducers able to mediate and/or convert different forms of energy into both physical and chemical cues, fostering specific cell behaviors. This report describes those classes of nanomaterials that have mostly paved the way to a "wireless" control of biological phenomena, focusing the discussion on some examples close to the clinical practice. In particular, magnetic fields, light irradiation, ultrasound, and pH will be presented as means to manipulate the cellular fate, due to the peculiar physical/chemical properties of some smart nanoparticles, thus providing realistic examples of "nanorobots" approaching the visionary ideas of Richard Feynman.

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Insight into the interactions between nanoparticles and cells

Cited by 84 publications

References 224 publications

Mesoporous silica nanoparticles trigger mitophagy in endothelial cells and perturb neuronal network activity in a size- and time-dependent manner

Mesoporous silica nanoparticles trigger mitophagy in endothelial cells and perturb neuronal network activity in a size- and time-dependent manner

Polyvalent Diazonium Polymers Provide Efficient Protection of Oncolytic Adenovirus Enadenotucirev from Neutralizing Antibodies while Maintaining Biological Activity In Vitro and In Vivo

Remote Control of Cellular Functions: The Role of Smart Nanomaterials in the Medicine of the Future

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