Insight into the effect of low temperature treatment on trichome density and related differentially expressed genes in Chinese cabbage

Abstract

Trichome is important for help plant resist adversity and external damage. However, it often affects the appearance and taste of vegetables. In the present study, the trichome density of leaves from two Chinese cabbage cultivars with and without trichomes treated at low temperature are analyzed by biological microscope, and the differentially expressed genes related to trichomes formation were screened through transcriptome sequencing. The results showed that the number of leaves trichomes was reduced by 34.7% at low temperature compared with room temperature. A total of 661 differentially expression genes effecting trichomes formation were identified at the CT vs C, LCT vs LC, CT vs LCT. Several differentially expression genes from every comparison group were enriched in plant hormone signal transduction and amino acid biosynthesis pathway. Combined with the central genes obtained by WGCNA analysis, five candidate genes Bra029778, Bra026393, Bra030270, Bra037264 and Bra009655 were screened. qRT-PCR analysis verified that the gene expression differences were in line with the trend of transcriptome data. This study not only found possible new key genes and laid a foundation for revealing the molecular mechanism regulating the formation of trichome in Chinese cabbage, but also provided a new way to study plant surface trichomes.

Light and temperature-dependent developmental role of Auxin Binding Protein 1 (ABP1) in Arabidopsis thaliana

“…As trichomes are known to be involved in several abiotic stresses (Wang et al, 2021) including cold tolerance, trichome density and architecture were studied in plant leaves grown under W light. As shown in previous studies, low-temperature stress resulted in varied alterations (either lower or higher) in trichome density (Sun et al 2022;Zhang et al, 2020 ) across different plant species. In the Col-0 line trichome density was increased by 2.9-fold under lower temperature (Figure 6A & 6B).…”

A Review on the Morphology, Cultivation, Identification, Phytochemistry, and Pharmacology of Kitagawia praeruptora (Dunn) Pimenov

“…But there is still a lack of systematic study in this area. At present, this study has confirmed that some exogenous hormones have a certain inhibitory effect on the early bolting of plants and at the same time can improve the quality of medicinal materials, which has been confirmed in plants such as A. sinensis [36], A. dahurica [37], and B. rapa [38]. In the study of genes related to bolting in K. praeruptora, researchers employed a combination of transcriptome sequencing and co-expression analysis to elucidate the regulatory mechanisms of key genes involved in coumarin synthesis and the formation of other active ingredients before and after bolting [39,40].…”