Insight into the B3Transcription Factor Superfamily and Expression Profiling of B3 Genes in Axillary Buds after Topping in Tobacco (Nicotiana tabacum L.)

Abstract

Members of the plant-specific B3 transcription factor superfamily play important roles in various growth and developmental processes in plants. Even though there are many valuable studies on B3 genes in other species, little is known about the B3 superfamily in tobacco. We identified 114 B3 proteins from tobacco using comparative genome analysis. These proteins were classified into four subfamilies based on their phylogenetic relationships, and include the ARF, RAV, LAV, and REM subfamilies. The chromosomal locations, gene structures, conserved protein motifs, and sub-cellular localizations of the tobacco B3 proteins were analyzed. The patterns of exon-intron numbers and arrangement and the protein structures of the tobacco B3 proteins were in general agreement with their phylogenetic relationships. The expression patterns of 114 B3 genes revealed that many B3 genes show tissue-specific expression. The expression levels of B3 genes in axillary buds after topping showed that the REM genes are mainly up-regulated in response to topping, while the ARF genes are down-regulated after topping.

PvB3s: Genome-wide and Transcriptome-wide Identification and Analysis Reveals the B3 Family Regulate Auxin to Resist Salt Stress at the Sprout Stage in Common Bean(P. Vulgaris L. ) .

“…This conclusion is consistent among citrus (C. sinensis and C. grandis) [36] and grapes (V. vinifera L.) [6].In tissue-speci c analysis, more PvB3s are expressed in the bud stage (whether ower buds, plant buds, or young pods) compared to other tissues. The same conclusion is found in grapes (V. vinifera L.) [37], citrus (C. sinensis and C. grandis) [36] and tobacco [38] (Nicotiana tabacum L.).…”

“…According to the domain structure and phylogenetic analysis, the B3 superfamily can be divided into four main families, namely, LAV (LEAFY COTYLE-DON2-ABAINSENSITIVE3-VAL), RAV (related to ABI3/VP1), ARF (auxin response factor) and REM (reproductive meristem) family. The B3 superfamily has found many model plants and crops in Arabidopsis, rice, poplar, rape, castor beans, cocoa, soybeans, corn, tobacco, grapes, moss, algae, citrus [3][4][5][6][7], but not foundings in common beans.…”

“…In previous studies, the genes of the B3 family have been reported in citrus (C. sinensis and C. grandis) [36], Savoy cabbage (B. oleracea) [37], and grapes (V. vinifera L.) [38], but no related articles have been reported in common beans. Through evolutionary tree analysis, the B3 transcription factor family has been divided into 4 Subgroups, and the motifs, cis elements, and gene structures within each sub-group are very similar, indicating that each sub-group has PvB3s with similar functions; but the PvB3s among the four subgroups are very large In the analysis of the B3 transcription factors of Arabidopsis and the common bean (P. vulgaris L.) two species, they are divided into 4 subgroups according to the genetic relationship, and the members in each subgroup are similar in terms of motif or gene structure.…”

Genome-wide analysis of the citrus B3 superfamily and their association with somatic embryogenesis

“…According to domain structure and phylogenetic analysis, the B3 superfamily is divided into four major families, namely the LAV (LEAFY COTYLEDON2-ABSCISIC ACID INSENSITIVE3-3 VAL), RAV (RELATED TO ABI3/VP1), ARF (AUXIN RESPONSE FACTOR) and REM (REPRODUCTIVE MERISTEM) families [1]. The B3 superfamily has been characterized in a number of model plants and crops, including Arabidopsis, rice, poplar, Brassica rapa, castor bean, cocoa, soybean, maize, tobacco, grapevine, moss and algae [1,[4][5][6][7], but not yet in citrus.…”

“…whereas genes in the other subgroup VAL (VAL1, VAL2 and VAL3) which also encode a CW-type zine finger, are expressed in many organs throughout plant development and have central roles in mediating repression of the LEC1/LEC2-ABI3 subgroup network during seed germination [1,6,11,18].…”