PvB3s: Genome-wide and Transcriptome-wide Identification and Analysis Reveals the B3 Family Regulate Auxin to Resist Salt Stress at the Sprout Stage in Common Bean(P. Vulgaris L. ) .

Abstract

<jats:title>Abstract</jats:title> <jats:p><jats:bold>Background:</jats:bold> <jats:italic>B3</jats:italic> gene family is a transcription factor family unique to plants, which play an important role in plant growth and development by binding specific DNA sequences. However, data on the <jats:italic>B3</jats:italic> genes in the common bean and participate in many abiotic stresses especially salt stress are limited. <jats:bold>Result:</jats:bold> A total of encoding 100 proteins were identified in common bean. Phylogenetic analysis showed that <jats:italic>PvB3s</jats:italic> were classified into 4 subgroups, and these clusters were supported by several group-specific features, including exon/intron structure, MEME motifs, and predicted binding site structure. Collinearity analysis showed the connection of <jats:italic>PvB3s</jats:italic> in the same species and different species. The genes expression pattern showed that <jats:italic>PvB3s</jats:italic> expressed with a tissue-specific manner during sprout stage. Through RNA-seq and qRT-PCR analysis, it was found that there were differences in expression in extreme materials under salt stress. The determination of auxin content and the analysis of <jats:italic>PvB3s</jats:italic> expression in the enriched pathway showed that <jats:italic>PvB3s</jats:italic> would respond to auxin to enhance salt tolerance in common bean sprouting stage. <jats:bold>Conclusion: </jats:bold>The results provided useful and rich resources of <jats:italic>PvB3s</jats:italic> for the functional characterization and understanding of <jats:italic>B3</jats:italic> transcription factors (TFs) in common bean, which further provides insights that <jats:italic>PvB3s</jats:italic> may respond to auxin to enhance salt tolerance of common bean.</jats:p>

Common bean as a potential crop for future food security: an overview of past, current and future contributions in genomics, transcriptomics, transgenics and proteomics

“…on the other hand, Phv-Arr-5, −8, −16 genes were up-regulated in Akman compared to Önceler. However, the change in the expression levels in the Phv-Why-2 gene in both varieties was determined as statistically significant compared to the rest of the genes under biotic stress Zhang et al [225]. studied the expression levels of six PvB3 genes under salt stress in cotyledon, hypocotyl, radicle, and some B3 family members revealed a relatively high expression in the radicle and hypocotyl.…”