Insight Into Metabolic Versatility of an Aromatic Compounds-Degrading Arthrobacter sp. YC-RL1

Ren, Lei; Yang, Jia; Zhang, Rui; Zhong, Liang; Zhen, Zhen; Hu, Hanqiao; Yan, Yanchun

doi:10.3389/fmicb.2018.02438

Cited by 23 publications

(15 citation statements)

References 68 publications

(81 reference statements)

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“…Aromatic compounds, widely found in the environment as components of plant materials and as pollutants of anthropogenic sources, are usually toxic to cellular systems and must be removed [ 1 ]. Benzoic acid is the simplest aromatic acid and a metabolic intermediate for many aromatic compounds [ 2 – 4 ]. Ecosystem pollution resulting from an increase in benzoic acid or sodium benzoate in soil and water poses a hazard to humans and other organisms in the ecosystem.…”

Section: Introductionmentioning

confidence: 99%

Complete Genome Sequence and Biodegradation Characteristics of Benzoic Acid-Degrading Bacterium Pseudomonas sp. SCB32

Xiang

Wei

Tang

et al. 2020

BioMed Research International

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Allelochemicals are metabolites produced by living organisms that have a detrimental effect on other species when released into the environment. These chemicals play critical roles in the problems associated with crop replanting. Benzoic acid is a representative allelochemical found in root exudates and rhizosphere soil of crops and inhibits crop growth. The bioremediation of allelochemicals by microorganisms is an efficient decontamination process. In this research, a bacterial strain capable of degrading benzoic acid as the sole carbon source was isolated. The genome of the strain was sequenced, and biodegradation characteristics and metabolic mechanisms were examined. Strain SCB32 was identified as Pseudomonas sp. based on 16S rRNA gene analysis coupled with physiological and biochemical analyses. The degradation rate of 800 mg L-1 benzoic acid by strain SCB32 was greater than 97.0% in 24 h. The complete genome of strain SCB32 was 6.3 Mbp with a GC content of 64.6% and 5960 coding genes. Potential benzoic acid degradation genes were found by comparison to the KEGG database. Some key intermediate metabolites of benzoic acid, such as catechol, were detected by gas chromatography-mass spectrometry. The biodegradation pathway of benzoic acid, the ortho pathway, is proposed for strain SCB32 based on combined data from genome annotation and mass spectrometry. Moreover, the benzoic acid degradation products from strain SCB32 were essentially nontoxic to lettuce seedlings, while seeds in the benzoic acid-treated group showed significant inhibition of germination. This indicates a possible application of strain SCB32 in the bioremediation of benzoic acid contamination in agricultural environments.

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Section: Introductionmentioning

confidence: 99%

Complete Genome Sequence and Biodegradation Characteristics of Benzoic Acid-Degrading Bacterium Pseudomonas sp. SCB32

Xiang

Wei

Tang

et al. 2020

BioMed Research International

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“…YC‐JH1 (Fan et al, 2018), A. keyseri 12B, Arthrobacter sp. YC‐RL1 (Ren et al, 2018) and Rhodococcus opacus 1CP (Eulberg, Lakner, Golovleva, & Schlömann, 1998), also bear PCA degradation‐related genes in their genomes.…”

Section: Resultsmentioning

confidence: 99%

Combined genomic and transcriptomic analysis of the dibutyl phthalate metabolic pathway in Arthrobacter sp. ZJUTW

Liu

Qiu

et al. 2020

Biotech & Bioengineering

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Dibutyl phthalate (DBP) is an environmental pollutant that can threaten human health. The strain Arthrobacter sp. ZJUTW, isolated from the sludge of a river of Hangzhou, can efficiently degrade DBP. Its genomic and transcriptomic differences when cultivated with DBP compared with glucose revealed specific DBP metabolic pathways in the ZJUTW strain. The degrading gene clusters localize separately on a circular chromosome and a plasmid pQL1. Genes related to the initial steps of DBP degradation from DBP to phthalic acid (PA), the pehA gene, and pht gene cluster, are located on the plasmid pQL1. However, the pca gene cluster related to the transforming of intermediate protocatechuic acid (PCA) to acetyl-CoA, is located on the chromosome. After comparative analysis with the reported gene clusters, we found that there were a series of homologous genes in pht and pca gene clusters that contribute to the efficient degradation of DBP by ZJUTW. In addition, transcriptomic analysis suggested a synergistic effect between pht and pca clusters, which also favor ZJUTW allowing it to efficiently degrade DBP. Combined genomic and transcriptomic analyses revealed a complete DBP metabolic pathway in Arthrobacter sp. ZJUTW that is different from that of other reported Arthrobacter strains. After necessary modification based on its metabolic characteristics, Arthrobacter sp. ZJUTW or its derivatives might represent promising candidates for the bioremediation of DBP pollution.

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“…The two isolates were mixed to form bacterial consortium CoPD. Both Arthrobacter and Rhodococcus were widely studied because of their properties of degradation for recalcitrant aromatic and pyridinic compounds (Arora and Sharma, 2015;Presentato et al, 2018;Ren et al, 2018;Sengupta et al, 2019;Sun et al, 2011;Yao et al, 2012;Zefirov et al, 1994). Compared with reported Arthrobacter and Rhodococcus, the co-culture CoPD showed more excellent pyridine degradation capacity.…”

Section: Discussionmentioning

confidence: 99%

Microbial Degradation of Pyridine by Co-culture of Two Newly Isolated Strains, Arthrobacter sp. Strain PDC-1 and Rhodococcus sp. Strain HPD-2

Hu¹,

Zhao²,

Guo³

et al. 2020

Preprint

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Pyridine is one of the most widespread heterocyclic contaminants. Microbial degradation of pyridine seems quite promising for its safety and efficiency. A bacterial consortium, which could use pyridine as the sole source of carbon, nitrogen, was obtained from the petroleum-contaminated soil from Liao River estuarine wetland. Two pyridine degrading strains, designated as PCD-1 and HPD-2, were isolated from the bacterial consortium. PCD-1 was identified as an Arthrobacter , and HPD-2 was identified as the Rhodococcus genus. The effects of pH, temperature, and pyridine concentration were investigated, and the optimum growth conditions for two strains were similar at pH 7.0 and 30°C. The co-culture of the two strains, CoPD, has better degradation efficiency compared with the individual strain. Haldane's inhibitory growth kinetics equation could be fitted to the growth of co-culture CoPD well for the entire concentration range. The kinetic constants obtained were μ max = 0.141 h -1 , K s = 37.9 mg/L, and K i = 3830 mg/L. Co-culture CoPD was able to remove more than 98% pyridine with an initial pyridine concentration of 5,000 mg/L within 120 h. Strain PCD-1 and HPD-2 have a novel pyridine degradation pathway different from the reported pathways. Major intermediates of pyridine degradation by two strains, including 2,5-pyrroldione, maleic semialdehyde, furanone, and butyrolactone, were identified using LC-MS analysis. CoPD is a promising tool for the treatment of wastewater containing pyridine, and this study contributes to the knowledge of the pyridine biodegradation by bacterial consortium.

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Insight Into Metabolic Versatility of an Aromatic Compounds-Degrading Arthrobacter sp. YC-RL1

Cited by 23 publications

References 68 publications

Complete Genome Sequence and Biodegradation Characteristics of Benzoic Acid-Degrading Bacterium Pseudomonas sp. SCB32

Complete Genome Sequence and Biodegradation Characteristics of Benzoic Acid-Degrading Bacterium Pseudomonas sp. SCB32

Combined genomic and transcriptomic analysis of the dibutyl phthalate metabolic pathway in Arthrobacter sp. ZJUTW

Microbial Degradation of Pyridine by Co-culture of Two Newly Isolated Strains, Arthrobacter sp. Strain PDC-1 and Rhodococcus sp. Strain HPD-2

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