Complete Genome Sequence and Biodegradation Characteristics of Benzoic Acid-Degrading Bacterium Pseudomonas sp. SCB32

Abstract

<jats:p>Allelochemicals are metabolites produced by living organisms that have a detrimental effect on other species when released into the environment. These chemicals play critical roles in the problems associated with crop replanting. Benzoic acid is a representative allelochemical found in root exudates and rhizosphere soil of crops and inhibits crop growth. The bioremediation of allelochemicals by microorganisms is an efficient decontamination process. In this research, a bacterial strain capable of degrading benzoic acid as the sole carbon source was isolated. The genome of the strain was sequenced, and biodegradation characteristics and metabolic mechanisms were examined. Strain SCB32 was identified as <jats:italic>Pseudomonas</jats:italic> sp. based on 16S rRNA gene analysis coupled with physiological and biochemical analyses. The degradation rate of 800 mg L<jats:sup>-1</jats:sup> benzoic acid by strain SCB32 was greater than 97.0% in 24 h. The complete genome of strain SCB32 was 6.3 Mbp with a GC content of 64.6% and 5960 coding genes. Potential benzoic acid degradation genes were found by comparison to the KEGG database. Some key intermediate metabolites of benzoic acid, such as catechol, were detected by gas chromatography-mass spectrometry. The biodegradation pathway of benzoic acid, the ortho pathway, is proposed for strain SCB32 based on combined data from genome annotation and mass spectrometry. Moreover, the benzoic acid degradation products from strain SCB32 were essentially nontoxic to lettuce seedlings, while seeds in the benzoic acid-treated group showed significant inhibition of germination. This indicates a possible application of strain SCB32 in the bioremediation of benzoic acid contamination in agricultural environments.</jats:p>

تثبيط تكوين الاغشية الحيوية في بكتيريا Agrobacterium tumefaciens بالمادة الطافية الخالية من خلايا Pseudomonas aeruginosa والمحللة بجهاز GC-MS

“…The great phenotypic difference shown by the isolated samples and the presence of other closely related species may lead to differences in the diagnosis of this type of bacteria when using traditional and molecular methods 35 . According to numerous studies about the identification of these bacteria 36,37 , our findings supported the integration of morphological features with biochemical characteristics and the genetic sequence of the 16S rRNA gene, confirming the diagnosis of the bacteria isolated from the soil as belonging to the genus Pseudomonas. This outcome is in line with what Eremwanarue et.…”

Autotoxicity in Panax notoginseng of root exudatesand their allelochemicals

“…The crop can be grown continuously on the same land for years when the inhibitory chemicals are removed. ( Gao and Wen, 2016 ; Xiang et al., 2020 ). Therefore, it is very important to understand the compositions of these inhibitory chemicals.…”

Dibenzofuran Degradation by Bacterial Community in Landfill Leachate

“…It has been shown in this study that the lowest dibenzofuran concentration (15 ppm) had shown the best concentration for maximum bacterial degradation of dibenzofuran in landfill leachate. Substrate availability represents the carbon sources the bacterial community uses to proliferate (Xiang et al, 2020). Lower substrate concentration might cause disability of the bacteria to proliferate, while in higher substrate concentration, accumulation of the by-products might inhibit bacterial growth to the extent that it is toxic to the bacteria cells (Maier & Pepper, 2015).…”