“…All of these bacterial genera and species are well-known PGPR (Farina et al 2012 ; Ahemad and Kibret 2014 ; Majeed et al 2015 ; Naqqash et al 2016 ) and common inhabitants of rhizosphere. The study also included endophytic strains from family Enterobacteriaceae, namely, E. cloacae , P. agglomerans, and C. sakazakii which are earlier reported to be siderophore producers (Mokracka et al 2004 ; Grim et al 2012 ; Walpola and Yoon 2013 ; Pandey et al 2016 ). A novel PGPR strain of Kosakonia pseudosacchari , which has not been reported to produce siderophore earlier, was also included in this study.…”
In this study, siderophore production by various bacteria amongst the plant-growth-promoting rhizobacteria was quantified by a rapid and efficient method. In total, 23 siderophore-producing bacterial isolates/strains were taken to estimate their siderophore-producing ability by the standard method (chrome azurol sulphonate assay) as well as 96 well microplate method. Production of siderophore was estimated in percent siderophore unit by both the methods. It was observed that data obtained by both methods correlated positively with each other proving the correctness of microplate method. By the modified microplate method, siderophore production by several bacterial strains can be estimated both qualitatively and quantitatively at one go, saving time, chemicals, making it very less tedious, and also being cheaper in comparison with the method currently in use. The modified microtiter plate method as proposed here makes it far easier to screen the plant-growth-promoting character of plant-associated bacteria.
“…All of these bacterial genera and species are well-known PGPR (Farina et al 2012 ; Ahemad and Kibret 2014 ; Majeed et al 2015 ; Naqqash et al 2016 ) and common inhabitants of rhizosphere. The study also included endophytic strains from family Enterobacteriaceae, namely, E. cloacae , P. agglomerans, and C. sakazakii which are earlier reported to be siderophore producers (Mokracka et al 2004 ; Grim et al 2012 ; Walpola and Yoon 2013 ; Pandey et al 2016 ). A novel PGPR strain of Kosakonia pseudosacchari , which has not been reported to produce siderophore earlier, was also included in this study.…”
In this study, siderophore production by various bacteria amongst the plant-growth-promoting rhizobacteria was quantified by a rapid and efficient method. In total, 23 siderophore-producing bacterial isolates/strains were taken to estimate their siderophore-producing ability by the standard method (chrome azurol sulphonate assay) as well as 96 well microplate method. Production of siderophore was estimated in percent siderophore unit by both the methods. It was observed that data obtained by both methods correlated positively with each other proving the correctness of microplate method. By the modified microplate method, siderophore production by several bacterial strains can be estimated both qualitatively and quantitatively at one go, saving time, chemicals, making it very less tedious, and also being cheaper in comparison with the method currently in use. The modified microtiter plate method as proposed here makes it far easier to screen the plant-growth-promoting character of plant-associated bacteria.
“…due to their ability to colonize internal plant tissues and live in the same ecological niches as pathogens. This capacity allows them to survive independently from external environmental factors while conferring economically “useful” properties in host plants [32,57,58]. For example, the introduction of endophytic bacteria ( B. subtilis 26D) into host plant tissues, before planting or during the vegetative phase, promoted plant (potato) growth, and protected plants from certain defects.…”
Section: Bacillus Spp Capacity To Alleviate Postharvest Losses Ofmentioning
confidence: 99%
“…Furthermore, Bacillus spp. are known to affect regulation of phytohormone biosynthesis pathways, modulate ethylene levels in plants, and influence the emission of volatile organic compounds (VOCs) and the launch of host plants’ systemic resistance/tolerance [7,9,11,14,24,27,31,32,33].…”
: Postharvest diseases significantly reduce the shelf-life of harvested fruits/vegetables worldwide. Bacillus spp. are considered to be an eco-friendly and bio-safe alternative to traditional chemical fungicides/bactericides due to their intrinsic ability to induce native anti-stress pathways in plants. This review compiles information from multiple scientific databases (Scopus, ScienceDirect, GoogleScholar, ResearchGate, etc.) using the keywords “postharvest diseases”, “Bacillus”, “Bacillus subtilis”, “biocontrol”, “storage”, “losses”, and “fruits/vegetables”. To date, numerous examples of successful Bacillus spp. application in controlling various postharvest-emerged pathogens of different fruits/vegetables during handling, transportation, and storage have been described in the literature. The mechanism/s of such action is/are still largely unknown; however, it is suggested that they include: i) competition for space/nutrients with pathogens; ii) production of various bio-active substances with antibiotic activity and cell wall-degrading compounds; and iii) induction of systemic resistance. With that, Bacillus efficiency may depend on various factors including strain characteristics (epiphytes or endophytes), application methods (before or after harvest/storage), type of pathogens/hosts, etc. Endophytic B. subtilis-based products can be more effective because they colonize internal plant tissues and are less dependent on external environmental factors while protecting cells inside. Nevertheless, the mechanism/s of Bacillus action on harvested fruits/vegetables is largely unknown and requires further detailed investigations to fully realize their potential in agricultural/food industries.
“…Due to the fact that as the most optimal treatments for the simultaneous suppression of late blight and fusarium were determined to be 10 8 CFU/mL (for strains 10-4 and 26D), 10 7 CFU/mL (for strain 10-4 + SA) and 10 6 CFU/mL (for strain 26D + SA) further microbiological, molecular, and physiological-biochemical studies would continue to use these established concentrations. Colonization of the internal tissues of plants by bacteria is one of the most important indicators of their endophytic properties and a factor influencing biological activity in plant-microbial relationships [25,43]. Using the prints of slices of surface sterilized tubers and quantitative accounting (titer B. subtilis), it was experimentally shown that B. subtilis 10-4 and 26D, when applied immediately prior to storage, effectively penetrate the internal tissues of the tubers and colonize them from the inside ( Figure 4A).…”
Section: Introductionmentioning
confidence: 99%
“…Using the prints of slices of surface sterilized tubers and quantitative accounting (titer B. subtilis), it was experimentally shown that B. subtilis 10-4 and 26D, when applied immediately prior to storage, effectively penetrate the internal tissues of the tubers and colonize them from the inside ( Figure 4A). Colonization of the internal tissues of plants by bacteria is one of the most important indicators of their endophytic properties and a factor influencing biological activity in plant-microbial relationships [25,43]. Using the prints of slices of surface sterilized tubers and quantitative accounting (titer B. subtilis), it was experimentally shown that B. subtilis 10-4 and 26D, when applied immediately prior to storage, effectively penetrate the internal tissues of the tubers and colonize them from the inside ( Figure 4A).…”
Postharvest diseases of potato lead to significant food and economic losses worldwide. The exogenous application of eco-friendly methods plays an important role in the control of postharvest decay. In this work the effects of endophytic bacteria B. subtilis (10-4, 26D) were studied in the context of two application parameters: concentration, with a range between 103–108 CFU/mL tested, and synergistic effects of the signal molecule salicylic acid (SA) (0.05 mM) on potato tubers’ resistance to Phytophthora infestans and Fusarium oxysporum during storage. The experiments were carried out on hydroponically grown potato (Solanum tuberosum L.) mini-tubers. This study demonstrates the suppressive effect of B. subtilis (10-4, 26D) on diseases of potato during storage and reveals that this effect happens in a dose-dependent manner, both individually and in combination with SA. The most effective concentrations of B. subtilis for suppression of both Ph. infestans and F. oxysporum are 108 CFU/mL (10-4 and 26D), 107 CFU/mL (10-4 + SA) and 106 CFU/mL (26D + SA). The ability of B. subtilis (10-4, 26D) to effectively penetrate and colonize the internal tubers’ tissues when applied immediately prior to storage, and the ability of SA to accelerate these processes, have been proven. B. subtilis (10-4, 26D), individually and in compositions with SA, increased ascorbic acid content and decreased pathogen-induced proline accumulation and lipid peroxidation in tubers. This indicates a protective effect conferred to cells against reactive oxygen and an extension of aging processes, manifested by a prolonged shelf life and extended preservation of fresh appearance.
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