Modified microplate method for rapid and efficient estimation of siderophore produced by bacteria

Arora, Naveen Kumar; Verma, Manisha

doi:10.1007/s13205-017-1008-y

Cited by 192 publications

(136 citation statements)

References 63 publications

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“…The supernatant (0.5 mL) of each actinobacterial isolate growing in LB broth at 28 • C for 5 days [32] was mixed with 0.5 mL of Chrome Azurol S (CAS) reagent. After 20 min of incubation at ambient temperature, the optical density of the test solution was measured at 630 nm [33]. The amount of siderophore produced was determined from a standard curve prepared with desferrioxamine mesylate as described by Baakza et al [34].…”

Section: Siderophore Estimationmentioning

confidence: 99%

“…Siderophores help to satisfy an organism's iron needs by chelating it from inorganic sources and making it available to both bacteria and plants [86,87]. Siderophore production is implicated in growth promotion and in biocontrol of phytopathogens [33]. Numerous strains of actinobacteria have been reported as siderophore producers [13].…”

Section: Pgp Potentials Of Extremophilic Actinobacteriamentioning

confidence: 99%

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Actinobacteria from Extreme Niches in Morocco and Their Plant Growth-Promoting Potentials

et al. 2019

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The objectives of this study were to assess actinobacterial diversity in five Moroccan extreme habitats and to evaluate their plant growth-promoting (PGP) activities. The soil samples were collected from different locations, including soils contaminated with heavy metals, from a high altitude site, from the desert, and from a marine environment. In total, 23 actinobacteria were isolated, 8 from Merzouga sand soil; 5 from Cannabis sativa rhizospheric soil; 5 from Toubkal mountain; 4 from a Draa sfar mining site; and 1 from marine soil. Based on their genotypic classification using 16S rRNA gene sequences, 19 of all belonged to the genus Streptomyces (82%) while the rest are the members of the genera Nocardioides (4.5%), Saccharomonospora (4.5%), Actinomadura (4.5%), and Prauserella (4.5%). Isolates Streptomyces sp. TNC-1 and Streptomyces sp. MNC-1 showed the highest level of phosphorus solubilization activity with 12.39 and 8.56 mg/mL, respectively. All 23 isolates were able to solubilize potassium, and 91% of them could grow under nitrogen-free conditions. The ability of the isolated actinobacteria to form indole-3-acetic acid (IAA) ranged from 6.70 to 75.54 μg/mL with Streptomyces sp. MNC-1 being the best IAA producer. In addition, all of the actinobacteria could produce siderophores, with Saccharomonospora sp. LNS-1 synthesizing the greatest amount (138.92 μg/mL). Principal coordinate analysis revealed that Streptomyces spp. MNC-1, MNT-1, MNB-2, and KNC-5; Saccharomonospora sp. LNS-1; and Nocardioides sp. KNC-3 each showed a variety of high-level plant growth-promoting activities. The extreme environments in Morocco are rich with bioactive actinobacteria that possess a variety of plant growth-promoting potentials that can further benefit green and sustainable agriculture.

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Section: Siderophore Estimationmentioning

confidence: 99%

Section: Pgp Potentials Of Extremophilic Actinobacteriamentioning

confidence: 99%

Actinobacteria from Extreme Niches in Morocco and Their Plant Growth-Promoting Potentials

et al. 2019

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“…To detect the production of IAA, isolate was grown in Czapexdox broth media and incubated at 28 °C for 72 h. IAA production was assayed colorimetrically using Salkowski's reagent (Bhagobaty and Joshi 2009). Siderophore production was qualitatively detected by the universal chrome-azurol S (CAS) assay according to Schwyn and Neilands (1987) and Arora and Verma (2017).…”

Section: Plant Growth Promoting Characteristicsmentioning

confidence: 99%

Role of peptaibols and lytic enzymes of Trichoderma cerinum Gur1 in biocontrol of Fusraium oxysporum and chickpea wilt

Khare

Kumar

Kim

2018

Environmental Sustainability

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Biocontrol of fungal phytopathogens is an important aspect of Trichoderma spp. In this study among fifty Trichoderma isolates, T. cerinum Gur1 showed best antagonistic activity against Fusarium oxysporum f. sp. ciceris (a major constraint to chickpea production worldwide) and was selected for understanding the biocontrol mechanism. The selected isolate Gur1 showed production of lytic enzymes (endoglucanase, chitinase and protease), peptaibol antibiotics, indole acetic acid (IAA), siderophore and phosphate solubilization activities. To detect the exact mechanism of biocontrol, SDS-PAGE analysis and chemical nature of biocontrol compound(s) was determined. SDS-PAGE analysis of enzyme sample from culture of Gur1 (grown in F. oxysporum cell wall supplemented medium) showed bands of 60, 29, and 42 kDa corresponding to β-1,3-glucanases, and chitinase, respectively. Effect of heat and protease treatment analysis showed that β-1,3-glucanases, chitinase and peptaibols play role in biocontrol of F. oxysporum. The photomicrograph of pathogen hyphae from the zone of inhibition also showed the function of both lytic enzymes and peptaibols in biocontrol. However, results clearly showed that the peptaibols have major role in biocontrol of F. oxysporum. In vivo results showed growth promotion and wilt disease suppression of chickpea by T. cerinum Gur1. Chickpea seeds treatment with Gur1 caused 13.33 and 58.33% enhancement in dry weight over control in absence and presence of F. oxysporum, respectively.

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“…The inoculation amount of 5% was connected to 250 mL SA medium at 28°C and 200 r/min cultured for 6 h, 12 h, 18 h, 24 h, 30 h, 36 h, 42 h, 48 h, 54 h and 60 h respectively. The growth density cell was measured by the spectrophotometer at OD 600 nm and CAS liquid detection method was used to initially detect the content of synthetic siderophore (Arora and Verma, 2017).…”

Section: Effect Of Culture Time On the Synthesis Of Siderophore By Anmentioning

confidence: 99%

“…The molecular weight is between 500-1500 Da and the siderophore specific transporter systems import the Fe-siderophore across the cell membrane to the cytoplasm (Fukushima et al, 2013). Indeed, there are more than 500 known species of siderophores and siderophores known species of siderophores and siderophores have been classified according to the Fe(III)-coordinating groups: Catecholate, hydroxamaces, carboxylates or mixed (Arora and Verma, 2017; Barry and Challis, 2009). At present, siderophore also can be classified by their mode of mode biosynthesis, Non-Ribosomal Peptide Synthetase (NRPS)-dependent or NRPS-independent biosynthesis (NIS) (Han et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Mining, Isolation and Identification of Siderophore Synthesis Gene from Brevibacillus brevis GZDF3

Sheng¹,

Jia²,

Xiaomai³

et al. 2018

American Journal of Biochemistry and Biotechnology

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Objective of this paper is to excavate the siderophore synthesis gene from Brevibacillus brevis GZDF3 and verify its type and antibacterial effects. The method is using genome mining technology to analyze the siderophore synthesis genes and the phylogenetic tree of each synthesis gene was constructed separately. Iron free medium was utilized to induce the synthesis of siderophore and CAS liquid detection method was used for qualitative and quantitative analysis on siderophore. The type of siderophore was preliminaries identified by Arnow and its antibacterial effects were analyzed according to the agar punching method. The results show that a siderophore synthesis gene cluster with 83% similarity to Petrobactin was found in Brevibacillus brevis GZDF3 genome. Iron free medium could induce siderophore synthesis and the optimal incubation time cultured in iron free medium was 30 h and 48 h. Antagonistic strain GZDF3 had the capacity to synthesize catechol-type siderophore. Also, GZDF3 had a powerful antibacterial effect on pathogenic fungus Fusarium oxysporum of rotted root on Pinellia ternata. Therefore, Brevibacillus brevis GZDF3 can produce catechol-type siderophore in an iron-deficient culture medium, which was also a main antifungal active substance.

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Modified microplate method for rapid and efficient estimation of siderophore produced by bacteria

Cited by 192 publications

References 63 publications

Actinobacteria from Extreme Niches in Morocco and Their Plant Growth-Promoting Potentials

Actinobacteria from Extreme Niches in Morocco and Their Plant Growth-Promoting Potentials

Role of peptaibols and lytic enzymes of Trichoderma cerinum Gur1 in biocontrol of Fusraium oxysporum and chickpea wilt

Mining, Isolation and Identification of Siderophore Synthesis Gene from Brevibacillus brevis GZDF3

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