Insecticide resistance alleles in wetland and residential populations of the West Nile virus vector<i>Culex pipiens</i>in New Jersey

Johnson, Brian J.; Fonseca, Dina M.

doi:10.1002/ps.4011

Cited by 3 publications

(1 citation statement)

References 64 publications

(143 reference statements)

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“…pipiens quinquefasciatus populations obtained from some parts of China ( 30 ) by L1014F allele in Cx. pipiens populations of New Jersey ( 31 ) and by L1014C in Cx. pipiens populations of China ( 32 ).…”

Section: Discussionmentioning

confidence: 99%

Insecticide Resistance and Mechanisms of Culex pipiens Populations in the Mediterranean and Aegean Regions of Turkey During 2017–2018

Yavaşoğlu¹,

Şimşek²

2022

JAD

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Background: Culex pipiens has a significant public health importance since it is an important vector of West Nile virus and Rift Valley fever virus. We, therefore, aimed to determine the in- secticide resistance level in Cx. pipiens populations in the Aegean and Mediterranean regions of Turkey. Methods: Bioassays have been carried out against Dichlorodiphenyltrichloroethane (DDT) (4%), Malathion (5%), Fenitrothion (1%), Propoxur (0.1%), Bendiocarb (0.1%), Permethrin (0.75%) and Deltamethrin (0.05%). Biochemical analyses have been performed to detect non-specific esterase, mixed function oxidase, glutathione-s-transferase and acetylcholinesterase levels. A knockdown resistance (kdr) (L1014F) and Acetylcholinesterase (Ace-1) (G119S) mutations have been detected by using allele-specific primers and a polymerase chain reaction (PCR) amplifica- tion of specific alleles (PASA) diagnostic test was performed for detection of F290V mutation. Results: Bioassay results showed that all Cx. pipiens populations were resistant to DDT, Mal- athion, Fenitrothion, Bendiocarb, Propoxur and some of the populations have started to gain Permethrin and Deltamethrin resistance. Biochemical analyses results revealed that altered glu- tathione-s-transferases, P450 monooxygenases, esterase levels might be responsible for DDT, or- ganophosphate, carbamate and pyrethroid resistance in Cx. pipiens populations. Results showed mild to high frequency of L1014F, low frequency of F290V but no Ace-1 G119S mutation within the populations. Additionally, acetylcholinesterase insensitivity was not significantly high within the most of these populations. Conclusion: Overall results may help to fulfil the lacking information in the literature regarding insecticide resistance status and underlying mechanism of Culex pipiens populations of the Mediter- ranean and Aegean region of Turkey by using all bioassays, molecular tests and biochemical assays.

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Section: Discussionmentioning

confidence: 99%

Insecticide Resistance and Mechanisms of Culex pipiens Populations in the Mediterranean and Aegean Regions of Turkey During 2017–2018

Yavaşoğlu¹,

Şimşek²

2022

JAD

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Mating behavior of the West Nile virus vector Culex pipiens – role of behavioral asymmetries

Benelli

2018

Acta Tropica

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Investigating insecticide resistance, kdr mutation, and morphology of the coastal mosquito Aedes (Ochlerotatus) zammitii (Theobald, 1903) (Diptera: Culicidae)

Bursalı

2024

TED

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This study aimed to assess the insecticide resistance levels and investigate the presence of the kdr mutation in Aedes (Ochlerotatus) zammitii (Theobald, 1903) (Diptera: Culicidae) mosquito populations collected from various locations within the Aegean region of Türkiye. Additionally, the study explored the morphological details of Ae. zammitii eggs and adults by using scanning electron microscopy (SEM). Mosquitoes were collected from their natural rocky breeding habitats from several provinces from April to October 2023 using larvae scoops at different aquatic stages. Emerged adult mosquitoes were identified using both taxonomic keys and molecular methods. The obtained images from SEM analysis revealed unique surface features that could potentially be used to identify the species. The susceptibility of adult Ae. zammitii to six insecticides, namely DDT (4%), fenitrothion (1%), bendiocarb (0.1%), deltamethrin (0.05%), permethrin (0.75%) and malathion (5%), was determined using the World Health Organization (WHO) susceptibility test. Results indicated that the mosquitoes exhibited varied possible resistance to the different pesticides tested. Mortality rates ranged between 72%-96%. This research confirmed the presence of kdr mutation associated with pyrethroid resistance in Ae. zammitii. The frequency of L1014F mutation ranged between 55 and 70% with the highest frequency determined in Antalya-Kaş population, followed by Karaburun and Çandarlı in İzmir. These findings significantly contribute to the understanding of insecticide resistance in Ae. zammitii, paving the way for developing effective mosquito control strategies in the Aegean region.

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Insecticide resistance alleles in wetland and residential populations of the West Nile virus vectorCulex pipiensin New Jersey

Cited by 3 publications

References 64 publications

Insecticide Resistance and Mechanisms of Culex pipiens Populations in the Mediterranean and Aegean Regions of Turkey During 2017–2018

Insecticide Resistance and Mechanisms of Culex pipiens Populations in the Mediterranean and Aegean Regions of Turkey During 2017–2018

Mating behavior of the West Nile virus vector Culex pipiens – role of behavioral asymmetries

Investigating insecticide resistance, kdr mutation, and morphology of the coastal mosquito Aedes (Ochlerotatus) zammitii (Theobald, 1903) (Diptera: Culicidae)

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