Insecticide pyriproxyfen (Dragón®) damage biotransformation, thyroid hormones, heart rate, and swimming performance of Odontophrynus americanus tadpoles

Lajmanovich, Rafael Carlos; Peltzer, Paola M.; Martinuzzi, Candela Soledad; Attademo, Andrés M.; Bassó, Agustín; Colussi, Carlina

doi:10.1016/j.chemosphere.2018.12.181

Cited by 34 publications

(19 citation statements)

References 64 publications

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“…In amphibian tadpoles, CbE contributes to the detoxification of GBH, considering that CbE probably binds to the phosphate fraction (Lajmanovich et al., 2011). Possibly, the CbE detoxification response at chronic exposure (NOEC-48h/8) to As (III) may be compensated with the induction of GST activity (Lajmanovich et al., 2019). GST is part of the first line of cellular defense, and is also used as a biomarker of pesticide exposure.…”

Section: Discussionmentioning

confidence: 99%

“…Total thyroid hormone levels were measured using enzyme-linked electro-chemiluminescent immunoassay (ECLIA) kits (COBAS®, Roche Diagnostics, Indianapolis, IN, USA) following that previously described in Lajmanovich et al. (2019).…”

Section: Methodsmentioning

confidence: 99%

“…Other authors (e.g. Gancedo et al., 1997; Li et al., 2016; Lajmanovich et al., 2019) have used this method.…”

Section: Methodsmentioning

confidence: 99%

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First evaluation of novel potential synergistic effects of glyphosate and arsenic mixture on Rhinella arenarum (Anura: Bufonidae) tadpoles

Peltzer

Attademo

Martinuzzi

et al. 2019

Heliyon

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The toxicity of glyphosate-based herbicide (GBH) and arsenite (As(III)) as individual toxicants and in mixture (50:50 v/v, GBH-As(III)) was determined in Rhinella arenarum tadpoles during acute (48 h) and chronic assays (22 days). In both types of assays, the levels of enzymatic activity [Acetylcholinesterase (AChE), Carboxylesterase (CbE), and Glutathione S-transferase (GST)] and the levels of thyroid hormones (triiodothyronine; T3 and thyroxine; T4) were examined. Additionally, the mitotic index (MI) of red blood cells (RBCs) and DNA damage index were calculated for the chronic assay. The results showed that the LC50 values at 48 h were 45.95 mg/L for GBH, 37.32 mg/L for As(III), and 30.31 mg/L for GBH-As(III) (with similar NOEC = 10 mg/L and LOEC = 20 mg/L between the three treatments). In the acute assay, Marking's additive index (S = 2.72) indicated synergistic toxicity for GBH-As(III). In larvae treated with GBH and As(III) at the NOEC-48h (10 mg/L), AChE activity increased by 36.25% and 33.05% respectively, CbE activity increased by 22.25% and 39.05 % respectively, and GST activity increased by 46.75% with the individual treatment with GBH and by 131.65 % with the GBH-As(III) mixture. Larvae exposed to the GBH-As(III) mixture also showed increased levels of T4 (25.67 %). In the chronic assay at NOEC-48h/8 (1.25 mg/L), As(III) and GBH-As(III) inhibited AChE activity (by 39.46 % and 35.65%, respectively), but did not alter CbE activity. In addition, As(III) highly increased (93.7 %) GST activity. GBH-As(III) increased T3 (97.34%) and T4 (540.93%) levels. Finally, GBH-As(III) increased the MI of RBCs and DNA damage. This study demonstrated strong synergistic toxicity of the GBH-As(III) mixture, negatively altering antioxidant systems and thyroid hormone levels, with consequences on RBC proliferation and DNA damage in treated R. arenarum tadpoles.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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First evaluation of novel potential synergistic effects of glyphosate and arsenic mixture on Rhinella arenarum (Anura: Bufonidae) tadpoles

Peltzer

Attademo

Martinuzzi

et al. 2019

Heliyon

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“…One explanation might be a lack of standardized methods and adequate endpoints to study these alterations. To our surprise, we found no ecotoxicological study in which automatic video tracking of exposed individuals was used in terrestrial amphibians, although this method is often used in a variety of taxa like bees [62], green lacewings [63] or mice [64] and also for aquatic amphibian larvae [65,66]. This method might provide informative endpoints in future terrestrial amphibian studies in an upcoming pesticide risk assessment for amphibians.…”

Section: Plos Onementioning

confidence: 99%

Avoidance behavior of juvenile common toads (Bufo bufo) in response to surface contamination by different pesticides

et al. 2020

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Most agricultural soils are expected to be contaminated with agricultural chemicals. As the exposure to pesticides can have adverse effects on non-target organisms, avoiding contaminated areas would be advantageous on an individual level, but could lead to a chemical landscape fragmentation with disadvantages on the metapopulation level. We investigated the avoidance behavior of juvenile common toads (Bufo bufo) in response to seven pesticide formulations commonly used in German vineyards. We used test arenas filled with silica sand and oversprayed half of each with different pesticide formulations. We placed a toad in the middle of an arena, filmed its behavior over 24 hours, calculated the proportion of time a toad spent on the contaminated side and compared it to a random side choice. We found evidence for the avoidance of the folpet formulation Folpan® 500 SC, the metrafenone formulation Vivando® and the glyphosate formulation Taifun® forte at maximum recommended field rates for vine and a trend for avoidance of Wettable Sulphur Stulln (sulphur). No avoidance was observed when testing Folpan® 80 WDG (folpet), Funguran® progress (copper hydroxide), SpinTorTM (spinosad), or 10% of the maximum field rate of any formulation tested. In the choice-tests in which we observed an avoidance, toads also showed higher activity on the contaminated side of the arena. As video analysis with tracking software is not always feasible, we further tested the effect of reducing the sampling interval for manual data analyses. We showed that one data point every 15 or 60 minutes results in a risk of overlooking a weak avoidance behavior, but still allows to verify the absence/presence of an avoidance for six out of seven formulations. Our findings are important for an upcoming pesticide risk assessment for amphibians and could be a template for future standardized tests.

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“…Several studies show that these agrochemicals affect the health of various nontarget organisms including amphibians living in the agroecosystems (Relyea, 2009). The biological effects of agrochemicals are chronic, detrimental and alter the biology of amphibians (Mann et al, 2009;Lajmanovich et al, 2019). Biomarker studies and other invasive methods dealing with the changes in the biology of frogs/tadpoles exposed to agrochemicals have revealed chromosomal aberrations, changes in the RBC structure etc., (Hegde and Krishnamurthy 2014;Josende et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Performance studies on jumping behaviour in froglets exposed to commercial grade malathion

Kulkarni¹,

Krishnamurthy²

2020

JEB

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Aim: Behavioural changes of an organism are used as an indicator to assess the impact of neurotoxic compounds. Jumping performance of newly emerged froglets exposed to malathion at their tadpole stage was studied. Methodology: Tadpole groups were exposed to 1226, 2453 and 6133 µg l-1 malathion in laboratory mesocosms. When they emerged as froglet, their jumping performance was studied. Results: As malathion is an AChE inhibitor and produces negative effect on survival of tadpoles, we anticipated increased malathion exposure could result in reduction of jumping distance in metamorphs. However, as compared to control, the jumping distance increased with malathion concentrations and showed significant increase in the individuals treated with the highest concentration of malathion (LC25; F3,21 = 11.41, p = 0.0001). Interpretation: Malathion is toxic to tadpoles; however, it could result in concentration dependent jumping performance within the tested concentrations. Several other factors like tadpole density, temperature of the media, pesticide tolerance may act as determining factor, which requires further investigations.

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Insecticide pyriproxyfen (Dragón®) damage biotransformation, thyroid hormones, heart rate, and swimming performance of Odontophrynus americanus tadpoles

Cited by 34 publications

References 64 publications

First evaluation of novel potential synergistic effects of glyphosate and arsenic mixture on Rhinella arenarum (Anura: Bufonidae) tadpoles

First evaluation of novel potential synergistic effects of glyphosate and arsenic mixture on Rhinella arenarum (Anura: Bufonidae) tadpoles

Avoidance behavior of juvenile common toads (Bufo bufo) in response to surface contamination by different pesticides

Performance studies on jumping behaviour in froglets exposed to commercial grade malathion

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