Insecticide Analysis, Colorimetric Determination of 1-Naphthyl N-Methylcarbamate in Agricultural Crops

Miskus, R.; Gordon, H. T.; George, Donald A. R.

doi:10.1021/jf60103a002

Cited by 50 publications

(17 citation statements)

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“…Since the final azo dye is coloured, the same reaction can be used at the level of the optical microscope and also in the biochemical assays of hydrolase activity. Its use in colorimetry has already been established (Miskus et al, 1959).…”

Section: Discussionmentioning

confidence: 99%

“…Other workers have demonstrated that 1-naphthol may be coupled with p-nitrobenzene diazonium-tetrafluoborate, and the coloured product has been employed as a chromatographic indicator and in colorimetry (Zweig & Archer, 1958;Miskus, Gordon & George, 1959;Krishna, Dorough & Casida, 1962;Johnson, 1963;Finocchiaro & Benson, 1967). Bowen & Lloyd (1971) showed that this coloured reaction product was insoluble and electron dense.…”

Section: Introductionmentioning

confidence: 99%

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A high resolution technique for the fine‐structural localization of acid hydrolases

Bowen

1971

Journal of Microscopy

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Summary An azo dye technique is described for the fine‐structural localization of acid hydrolases. Enzymatically released primary reaction product, an insoluble substituted naphthol, is coupled with ρ‐nitrobenzene diazonium‐tetrafluoborate to produce a dense particulate azo dye. Simultaneous and post‐coupling procedures are employed. The method is potentially applicable to all acid hydrolases and is free from the artifacts which beset the commonly used lead salt methods. The localization of β‐glucuronidase and acid phosphatase in rat and slug liver is specifically dealt with here. Lysosomal and cytoplasmic sources of enzyme activity are revealed.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A high resolution technique for the fine‐structural localization of acid hydrolases

Bowen

1971

Journal of Microscopy

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“…Methods used to determine residues after isolation from the poultry products are based on a modification (3) of the procedure described by hliskus et al for determining 1-naphthol by reaction with p-nitrobenzenediazonium fluoborate to produce an intense color (2). Free naphthol is determined directly in acetic acid medium.…”

Section: Methodsmentioning

confidence: 99%

Insecticide Residues in Meat and Eggs, Determination of Sevin Insecticide and Its Meabolites in Poultry Tissues and Eggs

Johnson¹,

Critchfield²,

Arthur³

1963

J. Agric. Food Chem.

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Colorimetric methods are described for determining Sevin and its metabolities, 1 -naphthol and 1 -naphthol conjugates, in poultry tissues and eggs. Free naphthol is reacted with pnitrobenzenediazonium fluoborate to produce a colored product which is measured spectrophotometrically. Sevin and 1 -naphthol conjugates are hydrolyzed to 1 -naphthol which is then determined in the same manner.Residue studies with poultry dusted with Sevin showed highest concentrations of residues to be in skin tissue where an average of 1 9.3 p.p.m. Sevin was found 1 day after the final treatment. A 7-day waiting period between treatment and slaughter reduced these residues to 2.15 p.p.m. No significant amount of either 1 -naphthol or its conjugates was found in any tissue of birds processed 7 days after treatment, and eggs were found free of residues throughout the study. Sevin(1-naphthyl '-methylcarbamate), a broad spectrum insecticide developed by Union Carbide Chemicals Co., has proved highly effective for controlling chicken mites, northern fowl mites, lice, and other ectoparasites of poultry (7). Control is gained by treating the litter in the cages or by direct application of the insecticide to the birds.To study the extent of contamination that may result in the edible tissues of birds treated with Sevin, a dusting experiment was conducted jointly between the Research and Development Department of Union Carbide Chemicals Co. and the Zoology-Entomology Department of Auburn University. The experiment was designed to provide residue data in all types of edible tissue for both the parent insecticide and its metabolite, 1-naphthol. Each type of tissue, including skin, dark and white muscle, liver, and gizzard, was analyzed separately to show' the distribution of residues in the carcass.

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“…The combined extracts were concentrated and the volume was made up to 100 ml. The residues were determined by the colorimetric method utilized by Gupta and Dewan (1973)-which is based on the method reported earlier for Carbaryl (Sevin) by Miskus et al (1959) and improved upon by Benson and Finocchiaro (1965).…”

Section: Extraction Clean-up and Residues Estimationmentioning

confidence: 99%

Persistence of aldicarb and carbofuran residues in potatoes grown in northwestern plains of India

Misra

Agrawal

1989

Tropical Pest Management

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Replicated field experiments were conducted during autumn 1982-83 at Jalandhar (Punjab) for evaluating an insecticidal schedule involving soil application of aldicarb (Temik) 10 G and carbofuran (Furadan) 3 G @ 1.0 and 1.5 kg a.i./ha in two different protocols viz.; (i) single full dose application of granules in furrows at planting and (ii) equal split dose application of granules (half in furrows at planting + remaining half dose near plants base at earthing up), found effective for aphid control, for residues. The findings indicate that aldicarb and carbofuran residues in unprocessed (unwashed, unboiled and unpeeled) potatoes from 1.0 and 1.5 kg a.i./ha doses applied in single full dose in furrows at planting (in the first protocol) persisted above the tolerance limits (0.50 ppm each for aldicarb and carbofuran) up to 70 days. Aldicarb residues in such unprocessed potatoes persisted above the tolerance limit up to 70/49 and 80/59 days respectively, from 1.0 and 1.5 kg a.i./ha doses when applied in two equal split doses at planting/earthing time. However, carbofuran residues in unprocessed potatoes were retained above the tolerance limit up to 70/49 days from both the doses applied in second protocol. At harvest, aldicarb residues in unprocessed potatoes were well within the tolerance limit from both the doses and protocols. On the other hand, carbofuran residues were undetectable from both the doses applied in the first protocol but were retained in traces (0.06 ppm) in unprocessed potatoes even from 1.0 kg a.i./ha dose applied in the second protocol. Residues of both the insecticides in processed (washed, boiled and peeled) potatoes and in water obtained after cooking the potatoes were below the tolerance limits in the beginning from both the doses and protocols which dissipated below the detectability level afterwards.

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Insecticide Analysis, Colorimetric Determination of 1-Naphthyl N-Methylcarbamate in Agricultural Crops

Cited by 50 publications

References 0 publications

A high resolution technique for the fine‐structural localization of acid hydrolases

A high resolution technique for the fine‐structural localization of acid hydrolases

Insecticide Residues in Meat and Eggs, Determination of Sevin Insecticide and Its Meabolites in Poultry Tissues and Eggs

Persistence of aldicarb and carbofuran residues in potatoes grown in northwestern plains of India

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