Insecticidal effects of an insect-specific neurotoxin expressed by a recombinant baculovirus

Maeda, Susumu; Volrath, Sandra; Hanzlik, Terry N.; Harper, Stephanie; Majima, Kei; Maddox, Daryl W.; Hammock, Bruce D.; Fowler, Elizabeth

doi:10.1016/0042-6822(91)90451-g

Cited by 133 publications

(60 citation statements)

References 16 publications

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“…2,3,4 and Tables 1 and 2). Previous studies reported that baculovirus-directed functional expression of the excitatory anti-insect selective scorpion toxin AaIT fused to a signal peptide coding sequences of an insect protein bombyxin or of the baculovirus protein gp67 [12][13][14]. In the present study, we demonstrate that expression, secretion and correct processing of LqhcdT can be achieved by direct cloning of its cDNA, including the LqhczIT signal peptide coding sequences.…”

Section: Discussionsupporting

confidence: 55%

Functional expression of an alpha anti‐insect scorpion neurotoxin in insect cells and lepidopterous larvae

et al. 1995

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Section: Discussionsupporting

confidence: 55%

Functional expression of an alpha anti‐insect scorpion neurotoxin in insect cells and lepidopterous larvae

et al. 1995

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“…Viruses, microorganisms, and algae.-Most genetic modification of viruses intended for environmental release has focused on baculoviruses (Cory 2000). Nontransgenic baculoviruses are already used for biological control of insects (Box 3), and researchers have tried to increase their efficiency by inserting genes that express toxic proteins, such as insect-specific scorpion toxins (Maeda et al 1991). To date, work with transgenic baculoviruses has taken place under contained conditions or in small-scale field tests, and no baculoviruses have been released commercially.…”

Section: Box 3 Biological Control Of Insects Using Ge Pathogensmentioning

confidence: 99%

GENETICALLY ENGINEERED ORGANISMS AND THE ENVIRONMENT: CURRENT STATUS AND RECOMMENDATIONS¹

Snow

Andow

Gepts

et al. 2005

Ecological Applications

290

216

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The Ecological Society of America has evaluated the ecological effects of current and potential uses of field‐released genetically engineered organisms (GEOs), as described in this Position Paper. Some GEOs could play a positive role in sustainable agriculture, forestry, aquaculture, bioremediation, and environmental management, both in developed and developing countries. However, deliberate or inadvertent releases of GEOs into the environment could have negative ecological effects under certain circumstances. Possible risks of GEOs could include: (1) creating new or more vigorous pests and pathogens; (2) exacerbating the effects of existing pests through hybridization with related transgenic organisms; (3) harm to nontarget species, such as soil organisms, non‐pest insects, birds, and other animals; (4) disruption of biotic communities, including agroecosystems; and (5) irreparable loss or changes in species diversity or genetic diversity within species. Many potential applications of genetic engineering extend beyond traditional breeding, encompassing viruses, bacteria, algae, fungi, grasses, trees, insects, fish, and shellfish. GEOs that present novel traits will need special scrutiny with regard to their environmental effects. The Ecological Society of America supports the following recommendations. (1) GEOs should be designed to reduce environmental risks. (2) More extensive studies of the environmental benefits and risks associated with GEOs are needed. (3) These effects should be evaluated relative to appropriate baseline scenarios. (4) Environmental release of GEOs should be prevented if scientific knowledge about possible risks is clearly inadequate. (5) In some cases, post‐release monitoring will be needed to identify, manage, and mitigate environmental risks. (6) Science‐based regulation should subject all transgenic organisms to a similar risk assessment framework and should incorporate a cautious approach, recognizing that many environmental effects are GEO‐ and site‐specific. (7) Ecologists, agricultural scientists, molecular biologists, and others need broader training and wider collaboration to address these recommendations. In summary, GEOs should be evaluated and used within the context of a scientifically based regulatory policy that encourages innovation without compromising sound environmental management. The Ecological Society of America is committed to providing scientific expertise for evaluating and predicting the ecological effects of field‐released transgenic organisms.

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“…A well-documented example of a recombinant baculovirus is that carrying the sequence of the toxin from A. australis Hector scorpion venom [46,47]. Several recombinant baculoviruses with different toxin synthetic genes have been studied in the laboratory [6,48,49] and in controlled conditions in the field [47,50].…”

Section: Discussionmentioning

confidence: 99%

Poneratoxin, a neurotoxin from ant venom

Szołajska

Poznański

Ferber

et al. 2004

European Journal of Biochemistry

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Poneratoxin is a small neuropeptide found in the venom of the ant Paraponera clavata. It is stored in the venom reservoir as an inactive 25-residue peptide. Here we describe both chemically synthesized poneratoxin and poneratoxin obtained by expression in insect cells. When expressed in insect cells, poneratoxin was observed attached to cell membranes. Both synthetic and recombinant ponerotoxins were soluble below pH 4.5. The structure of synthetic poneratoxin was characterized by circular dichroism and solved by nuclear magnetic resonance. In an environment imitating a lipid bilayer, at pH within the range of insect hemolymph, synthetic poneratoxin has a V shape, with two a-helices connected by a b-turn. Insect larvae were paralyzed by injection of either of the purified toxins, with the recombinant one acting faster. The recombinant toxin-producing baculovirus reduced the average survival time of the insect host by 25 h compared with unmodified virus. Mass spectrometry analysis showed that the recombinant toxin has an N-terminal 21-residue extension, possibly improving its stability and/or stabilizing the membrane-bound state. The potential use of poneratoxin for the construction of biological insecticide is discussed.

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Insecticidal effects of an insect-specific neurotoxin expressed by a recombinant baculovirus

Cited by 133 publications

References 16 publications

Functional expression of an alpha anti‐insect scorpion neurotoxin in insect cells and lepidopterous larvae

Functional expression of an alpha anti‐insect scorpion neurotoxin in insect cells and lepidopterous larvae

GENETICALLY ENGINEERED ORGANISMS AND THE ENVIRONMENT: CURRENT STATUS AND RECOMMENDATIONS¹

Poneratoxin, a neurotoxin from ant venom

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Insecticidal effects of an insect-specific neurotoxin expressed by a recombinant baculovirus

Cited by 133 publications

References 16 publications

Functional expression of an alpha anti‐insect scorpion neurotoxin in insect cells and lepidopterous larvae

Functional expression of an alpha anti‐insect scorpion neurotoxin in insect cells and lepidopterous larvae

GENETICALLY ENGINEERED ORGANISMS AND THE ENVIRONMENT: CURRENT STATUS AND RECOMMENDATIONS1

Poneratoxin, a neurotoxin from ant venom

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GENETICALLY ENGINEERED ORGANISMS AND THE ENVIRONMENT: CURRENT STATUS AND RECOMMENDATIONS¹