Insecticidal activity of three species of Guatteria (Annonaceae) against Aedes aegypti (Diptera: Culicidae)

Abstract

The products of vegetal origin were assessed for bioactive substances to reduce reliance on organophosphate and pyrethroid insecticides, to which insect populations have become resistant. For this reason the aim of this study was to assess whether the essential oils of Guatteria hispida, G. blepharophylla and G. friesiana have insecticidal effect against A. aegypti under laboratory conditions. Essential oils were extracted through hydrodistillation using a modified Clevenger apparatus and analyzed by Gas Chromatography (CG-FID), Gas Chromatography coupled to Mass Spectrometry (GC-MS), and Nuclear Magnetic Resonance (NMR). the bioassays were analyzed according to the Probit model. The GC-MS and NMR analyses confirmed that the leaves of G. blepharophylla have the caryophyllene oxide as their main component; in G. friesiana the -,and -eudesmols prevail, and in G. hispida and -pinene, and (E)-caryophyllene are the predominant compounds. the lethal concentrations LC50, LC95 and LC99, were respectively 85.74, 199.35 and 282.76ppm for G. hispida; 58.72, 107.6 and 138.37ppm for G. blepharophylla; and 52.6, 94.37 and 120.22ppm for G. friesiana. the oil extracted from G. friesiana presented the best insecticidal effect.

7,7-Dimethylaporphine and Other Alkaloids from the Bark of Guatteria friesiana

“…Some of them are known for their medicinal properties, as supported by previous investigations revealing bioactive compounds showing potential anticancer, − antimicrobial, ,,− antioxidant, and antiparasitic activities. , Guatteria friesiana (W.A. Rodrigues) Erkens & Maas is known popularly as “envireira” or “envira”, and previous phytochemical investigations have described the chemical constituents of its essential oil ,, as well as aporphine alkaloids found in its leaves and stem. , In a continuing search for bioactive compounds from Amazonian annonaceous plants, 12 new ( 1 – 12 ) and nine previously described ( 13 – 21 ) alkaloids were obtained in a systematic activity-guided investigation of the bark of G. friesiana .…”

Essential Oils of Three Balkan Abies Species: Chemical Profiles, Antimicrobial Activity and Toxicity toward Artemia salina and Drosophila melanogaster

“…This might be explained by the highest concentrations of several sesquiterpene compounds in A . × borisii‐regis EO, such as ( E )‐caryophyllene (8.0 %), α‐humulene (4.5 %), and 10‐ epi ‐γ‐eudesmol (4 %), which were described as potent insecticidal agents against Megoura japonica and Plutella xylostella , [33] Helicoverpa armigera [34] and Aedes aegypti , [35] respectively. On the other hand, although A. alba EO was characterized by the highest concentrations of camphene and limonene, the toxicity of this EO toward A. salina was significantly weaker compared to the other two Abies EOs, which may indicate a smaller contribution of these two components to the overall toxicity of the EOs.…”

“…[18] On the other hand, A. × borisii-regis EO had the highest concentration of some sesquiterpene compounds such as (E)-caryophyllene (8.0 %) and α-humulene (4.5 %), which were described as potent insecticidal agents for different lepidopteran and hymenopteran pest species, [33,34] as well as 10-epi-γ-eudesmol (4 %) against Aedes aegypti, [35] which is a dipteran insect just like D. melanogaster. In addition, toxic effect on D. melanogaster of P. sylvestris EO was suggested to be caused by high concentration of (E)-caryophyllene in that EO.…”