Insect-Resistant Transgenic Brassicas

Earle, Elizabeth D.; Cao, Jun; Shelton, Anthony M.

doi:10.1007/978-3-662-06164-0_12

Cited by 5 publications

(4 citation statements)

References 55 publications

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“…Delaying larval growth and development can be a potential pest management strategy for controlling crucifer pests. Crucifer pests are also targets of proposed transgenic plant protection strategies that target the insect midgut, including Bacillus thuringiensis (Bt) endotoxins genes and genes for inhibitors of midgut digestive enzymes (Sharma et al, 2000 ; Earle et al, 2004 ; Shelton et al, 2009 ; Schlüter et al, 2010 ; Zibaee, 2012 ; Zibaee et al, 2014 ). This study was undertaken to contribute knowledge toward development of environmentally safe, sustainable strategies for control of P. brassicae using protease inhibitors for the region.…”

Section: Discussionmentioning

confidence: 99%

Midgut serine proteases and alternative host plant utilization in Pieris brassicae L.

et al. 2015

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Pieris brassicae L. is a serious pest of cultivated crucifers in several parts of the world. Larvae of P. brassicae also feed prolifically on garden nasturtium (Tropaeolum majus L., of the family Tropaeolaceae). Proteolytic digestion was studied in larvae feeding on multiple hosts. Fourth instars were collected from cauliflower fields before transfer onto detached, aerial tissues of selected host plants in the lab. Variable levels of midgut proteases were detected in larvae fed on different hosts using protein substrates (casein and recombinant RBCL cloned from cauliflower) and diagnostic, synthetic substrates. Qualitative changes in midgut trypsin activities and quantitative changes in midgut chymotrypsin activities were implicated in physiological adaptation of larvae transferred to T. majus. Midgut proteolytic activities were inhibited to different extents by serine protease inhibitors, including putative trypsin inhibitors isolated from herbivore-attacked and herbivore-free leaves of cauliflower (CfTI) and T. majus (TpTI). Transfer of larvae to T. majus significantly influenced feeding parameters but not necessarily when transferred to different tissues of the same host. Results obtained are relevant for devising sustainable pest management strategies, including transgenic approaches using genes encoding plant protease inhibitors.

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Section: Discussionmentioning

confidence: 99%

Midgut serine proteases and alternative host plant utilization in Pieris brassicae L.

et al. 2015

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“…Effective insect control has also been achieved in Bt-transgenic crucifer vegetable, although no such products have yet been released commercially because of economic, environmental and social concerns. Nevertheless, such concerns may eventually be resolved, allowing the use of Bt crucifers for insect pest control (Earle et al 2004;Cao et al 2005). The use of Bt could lead to the development of Bt resistance in pest populations which influenced the efficacy of using Bt transgenic vegetable and other Bt treatments.…”

Section: Discussionmentioning

confidence: 99%

“…Effective insect control has also been achieved in Bt‐transgenic crucifer vegetable, although no such products have yet been released commercially because of economic, environmental and social concerns. Nevertheless, such concerns may eventually be resolved, allowing the use of Bt crucifers for insect pest control (Earle et al. 2004; Cao et al.…”

Section: Discussionmentioning

confidence: 99%

Monitoring of resistance for the diamondback moth to Bacillus thuringiensis Cry1Ac and Cry1Ba toxins and a Bt commercial formulation

Wang

Zhang

et al. 2007

J Applied Entomology

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To monitor the resistance of field populations of the diamondback moth Plutella xylostella in China to the insecticidal protein Cry1Ac, Cry1Ba and commercial formulation Bacillus thuringiensis var. kurstaki (Btk), six representative populations of the diamondback moth were collected from Shanghai, Shandong, Hubei, Hunan, Zhejiang and Guangdong provinces of China where crucifer crop plants are intensively planted. Bioassay results showed that the populations of the diamondback moth from different locations exhibited different levels of resistance, compared with a susceptible laboratory population. The Guangdong field population was 56.15-and 21.90-fold resistant to Cry1Ac and Btk, respectively. Shanghai, Hunan, Shandong and Zhejiang populations were 37.85-, 17.24-, 10.24-and 9.41-fold resistant to Cry1Ac, respectively, but were not resistant to Btk. The Hubei population did not show resistance to Cry1Ac and Btk. Almost all tested populations were susceptible to Cry1Ba, but the Guangdong population showed some tolerance to Cry1Ba with a LC 50 of 0.69 lg/ml which was 6.17-fold higher than that of the susceptible population. The results suggested that the complex resistance patterns of field populations of P. xylostella need to be considered for expression of Bt toxin genes in genetically-engineered crop plants and commercial formulations.

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“…The major insect that attacks these vegetables is the diamond back moth (DBM; Plutella xylostella). Several Bt genes have been introduced for conferring resistance to DBM and other Lepidoptera (Earle et al 2004;Paul et al 2005). Synthetic cry1C was transferred to broccoli against Plutella (Cao et al 1999) and later pyramided with cry1A gene (Cao et al 2002).…”

Section: Heliothis Obsoleta Liriomyza Trifoliimentioning

confidence: 99%