İnsan Kanser Hücrelerinde Astragalus elongatus subsp. nucleiferus’ un Antikanser, Antiproliferatif ve Laktat Dehidrojenaz Enzim Aktiviteleri

Abstract

In this study, potential anticancer and antiproliferative activities of ethanol and water extracts from aerial parts and roots of Astragallus elongatus subsp. nucleiferus were evaluated. MTT, trypan blue, and LDH enzyme activity assays were performed to determine cytotoxicity and cell proliferation potentials of the plant extracts against human A549, H1299, C6 cancer cells, and non-tumorous HUVECs. As results of MTT and trypan blue assays, dose-dependent anticancer and antiproliferative effects were observed on tested human lung and brain carcinoma cells. The water extracts obtained from the root exerted higher anticancer activity with IC50 values ranging from 5.81±0.46 to 18.24±0.12µg/mL than the extracts of aerial parts. In contrary anticancer and cytotoxicity results, the ethanol extract of the root was also caused to the highest apoptosis level in a dose dependently. Regarding LDH activity, the plant extracts obtained from aerial parts and roots were demonstrated the highest LDH activity towards H1299 cells. The obtained results showed that the roots of the plant are able to inhibit cell growth in cancer cells in a time and concentration dependently.