Inorganic delivery vector for intravenous injection

Kwak, Seo Young; Kriven, Waltraud M.; Wallig, Matthew A.; Choy, Jin‐Ho

doi:10.1016/j.biomaterials.2004.01.056

Cited by 140 publications

(100 citation statements)

References 13 publications

(24 reference statements)

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“…Over recent years clay-based nanoparticles, layered double hydroxides (LDHs), have emerged as a siRNA delivery system with the potential to fulfill the above criteria. LDHs ([Mg n Al(OH) 2n+2 ] + (A -)mH 2 O, where A = Cl -or NO 3 -, n = 2-3, m = 2) have been convincingly shown to efficiently transport a range of anionic drugs, including siRNAs, into mammalian cells [8][9][10][11][12][13][14][15][16]. Structurally, LDHs comprise alternating stacks of positively charged brucite-like layers ([Mg n Al(OH) 2n+2 ] + ), neutralized by exchangeable interlayer hydrated anions ((A -)mH 2 O).…”

Section: Introductionmentioning

confidence: 99%

Reduction in the size of layered double hydroxide nanoparticles enhances the efficiency of siRNA delivery

Chen

Cooper

Zhou

et al. 2013

Journal of Colloid and Interface Science

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Small interfering RNAs (siRNA) are a potentially powerful new class of pharmaceutical drugs for many diseases, while the delivery of unprotected siRNAs is ineffective due to their susceptibility to degradation by ubiquitous nucleases under physiological conditions. Layered double hydroxide nanoparticles (LDHs) have been found to be efficient carriers of anionic drugs and nucleic acids. Our previous research has shown that LDHs (with the Z-average particle size of approximately 110 nm) can mediate siRNA delivery in mammalian cells, resulting in gene silencing. However, short double-stranded nucleic acids are mostly adsorbed onto the external surface and not well protected by LDHs. In order to enhance the intercalation of siRNA into the LDH interlayer and the efficiency of subsequent siRNA delivery, we prepared smaller LDHs (with the Z-average particle size of approximately 45 nm) with an engineered nonaqueous method. We demonstrate here that dsDNA/siRNA is more effectively intercalated into these small LDH nanoparticles, more dsDNA/siRNA is transfected into HEK 293T cells, and more efficient silencing of the target gene is achieved using smaller LDHs. Thus smaller LDH particles have greater potential as a delivery system for the application of RNA interference.

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Section: Introductionmentioning

confidence: 99%

Reduction in the size of layered double hydroxide nanoparticles enhances the efficiency of siRNA delivery

Chen

Cooper

Zhou

et al. 2013

Journal of Colloid and Interface Science

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“…[3][4][5] In recent years, layered double hydroxide (LDH), which is nothing different from anionic clay with a hydrotalcite structure, has attracted an increasing amount of interest as a delivery nanovehicle owing to its high biocompatibility, controllable drug content, enhanced cellular permeation property, and ability to overcome drug resistance. [6][7][8][9][10][11][12] : anionic molecules, 0,x,1) and consists of positively charged layers and interlayer anions that are alternately stacked with each other along the crystallographic c-axis to form a 1:1 type heterostructure. 13 The positive layer charge in LDH can be easily controlled by replacing the divalent cation, M(II), in the lattice partially with the trivalent one, M(III), in a way in which the negatively charged drug or biomolecules can be stabilized in the interlayer space of LDH to compensate for the positive layer charge.…”

Section: Introductionmentioning

confidence: 99%

Anionic clay as the drug delivery vehicle: tumor targeting function of layered double hydroxide-methotrexate nanohybrid in C33A orthotopic cervical cancer model

Choi¹,

Piao²,

ALOthman³

et al. 2016

IJN

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Methotrexate (MTX), an anticancer agent, was successfully intercalated into the anionic clay, layered double hydroxides to form a new nanohybrid drug. The coprecipitation and subsequent hydrothermal method were used to prepare chemically, structurally, and morphologically well-defined two-dimensional drug-clay nanohybrid. The resulting two-dimensional drug-clay nanohybrid showed excellent colloidal stability not only in deionized water but also in an electrolyte solution of Dulbecco’s Modified Eagle’s Medium with 10% fetal bovine serum, in which the average particle size in colloid and the polydispersity index were determined to be around 100 and 0.250 nm, respectively. The targeting property of the nanohybrid drug was confirmed by evaluating the tumor-to-blood and tumor-to-liver ratios of the MTX with anionic clay carrier, and these ratios were compared to those of free MTX in the C33A orthotopic cervical cancer model. The biodistribution studies indicated that the mice treated with the former showed 3.5-fold higher tumor-to-liver ratio and fivefold higher tumor-to-blood ratio of MTX than those treated with the latter at 30 minutes postinjection.

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“…Their low cost, good biocompatibility, low toxicity to mammalian cells, controlled-release system, and ability to provide full protection for loaded drugs make them suitable candidates for drug delivery systems. 11,12 Many LDH compounds intercalated with beneficial organic anions, such as deoxyribonucleic acid DNA, [13][14][15][16] amino acids, [17][18][19][20][21][22] anionic polymers, 23 pesticides, 24,25 and drugs, [26][27][28][29][30] have been prepared successfully. Special interests have focused on exploring the possibilities of using LDH drug delivery systems to deliver antiinflammatory drugs, 31 anticoagulants like heparin, 32 or anticancer drugs.…”

Section: Introductionmentioning

confidence: 99%

The in vitro sustained release profile and antitumor effect of etoposide-layered double hydroxide nanohybrids

Qin¹,

Wang²,

Zhu³

et al. 2013

IJN

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Magnesium-aluminum layered double hydroxides intercalated with antitumor drug etoposide (VP16) were prepared for the first time using a two-step procedure. The X-ray powder diffraction data suggested the intercalation of VP16 into layers with the increased basal spacing from 0.84-1.18 nm was successful. Then, it was characterized by X-ray powder diffraction, Fourier transform infrared spectroscopy, thermogravimetry and differential thermal analysis, and transmission electron microscopy. The prepared nanoparticles, VP16-LDH, showed an average diameter of 62.5 nm with a zeta potential of 20.5 mV. Evaluation of the buffering effect of VP16-LDH indicated that the nanohybrids were ideal for administration of the drugs that treat human stomach irritation. The loading amount of intercalated VP16 was 21.94% and possessed a profile of sustained release. The mechanism of VP16-LDH release in the phosphate buffered saline solution at pH 7.4 is likely controlled by the diffusion of VP16 anions from inside to the surface of LDH particles. The in vitro cytotoxicity and antitumor assays indicated that VP16-LDH hybrids were less toxic to GES-1 cells while exhibiting better antitumor efficacy on MKN45 and SGC-7901 cells. These results imply that VP16-LDH is a potential antitumor drug for a broad range of gastric cancer therapeutic applications.

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Inorganic delivery vector for intravenous injection

Cited by 140 publications

References 13 publications

Reduction in the size of layered double hydroxide nanoparticles enhances the efficiency of siRNA delivery

Reduction in the size of layered double hydroxide nanoparticles enhances the efficiency of siRNA delivery

Anionic clay as the drug delivery vehicle: tumor targeting function of layered double hydroxide-methotrexate nanohybrid in C33A orthotopic cervical cancer model

The in vitro sustained release profile and antitumor effect of etoposide-layered double hydroxide nanohybrids

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