The in vitro sustained release profile and antitumor effect of etoposide-layered double hydroxide nanohybrids

Abstract

Magnesium-aluminum layered double hydroxides intercalated with antitumor drug etoposide (VP16) were prepared for the first time using a two-step procedure. The X-ray powder diffraction data suggested the intercalation of VP16 into layers with the increased basal spacing from 0.84-1.18 nm was successful. Then, it was characterized by X-ray powder diffraction, Fourier transform infrared spectroscopy, thermogravimetry and differential thermal analysis, and transmission electron microscopy. The prepared nanoparticles, VP16-LDH, showed an average diameter of 62.5 nm with a zeta potential of 20.5 mV. Evaluation of the buffering effect of VP16-LDH indicated that the nanohybrids were ideal for administration of the drugs that treat human stomach irritation. The loading amount of intercalated VP16 was 21.94% and possessed a profile of sustained release. The mechanism of VP16-LDH release in the phosphate buffered saline solution at pH 7.4 is likely controlled by the diffusion of VP16 anions from inside to the surface of LDH particles. The in vitro cytotoxicity and antitumor assays indicated that VP16-LDH hybrids were less toxic to GES-1 cells while exhibiting better antitumor efficacy on MKN45 and SGC-7901 cells. These results imply that VP16-LDH is a potential antitumor drug for a broad range of gastric cancer therapeutic applications.

Inorganic nanolayers: structure, preparation, and biomedical applications

“…[123][124][125][126][127] Qin et al prepared a nanohybrid formulation of VP16 by intercalating it into Mg/Al LDHs (VP16-Mg/Al LDHs). 128 They found that the nanohybrid (VP16-Mg/Al LDHs) possessed better antitumor activity against MKN45 and SGC-7901 cells. The release of VP16 from LDHs was found to be sustained and followed a diffusion mechanism, as it followed the parabolic kinetic model.…”

Etoposide-loaded nanostructured lipid carriers for gastric cancer therapy

“…Etoposide (ETP), a semisynthetic derivative of podophyllotoxin, is the inhibitor of deoxyribonucleic acid (DNA) topoisomerase 2 (Qin et al, 2013). Its main effect appears to be at the G 2 portion of the cell cycle in mammalian cells.…”

Intracellular uptake of etoposide-loaded solid lipid nanoparticles induces an enhancing inhibitory effect on gastric cancer through mitochondria-mediated apoptosis pathway

“…5 However, owing to low solubility, drug resistance, and poor bioavailability, the therapeutic efficacy of VP16 is limited. [6][7][8] Therefore, an efficient drug delivery system is desired to overcome these drawbacks and improve the clinical therapy effects.…”