Inoculation of newly hatched broiler chicks with two Brazilian isolates of Salmonella Heidelberg strains with different virulence gene profiles, antimicrobial resistance, and pulsed field gel electrophoresis patterns to intestinal changes evaluation

Borsoi, Anderlise; Santín, Elizabeth; Santos, Luciana Ruschel dos; Salle, Carlos Tadeu Pippi; Moraes, H. L. S.; Nascimento, Vladimir Pinheiro do

doi:10.3382/ps.2008-00466

Cited by 46 publications

(41 citation statements)

References 30 publications

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“…The high frequency of lpfA and agfA were similar to other data obtained by previous works that studied different serovars , Borsoi et al 2009, Cesco 2010. Besides being important in the adhesion during the infection process, the agfA gene is also associated with biofilm formation (Barnhart & Chapman 2006, Yoo et al 2013.…”

Section: Discussionsupporting

confidence: 80%

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Detection of virulence-associated genes in Salmonella Enteritidis isolates from chicken in South of Brazil

et al. 2013

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RESUMO.-[Detecção de genes associados à virulência em cepas de Salmonella Enteritidis isoladas de frangos na região sul do Brasil.] Salmonella spp. estão entre os principais agentes causadores de doenças transmitidas por alimentos, e o sorovar Salmonella Enteritidis é o mais frequentemente isolado no mundo. A virulência de Salmonella spp. e a sua interação com o hospedeiro são processos complexos que envolvem fatores de virulência para sobreviver às defesas do hospedeiro. O objetivo deste estudo foi detectar genes de virulência em cepas de S. Enteritidis isoladas a partir de fontes avícolas no sul do Brasil. Ensaios de PCR foram desenvolvidos para a detecção de nove genes (lpfA, agfA, sefA, invA, hilA, avrA, sopE, sivH are considered the main agents of foodborne disease and Salmonella Enteritidis is one of the most frequently isolated serovars worldwide. The virulence of Salmonella spp. and their interaction with the host are complex processes involving virulence factors to overcome host defenses. The purpose of this study was to detect virulence genes in S. Enteritidis isolates from poultry in the South of Brazil. PCR-based assays were developed in order to detect nine genes (lpfA, agfA, sefA, invA, hilA, avrA, sopE, sivH and spvC) associated with the virulence in eighty-four isolates of S. Enteritidis isolated from poultry. The invA, hilA, sivH, sefA and avrA genes were present in 100% of the isolates; lpfA and sopE were present in 99%; agfA was present in 96%; and the spvC gene was present in 92%. It was possible to characterize the isolates with four different genetic profiles (P1, P2, P3 and P4), as it follows: P1, positive for all genes; P2, negative only for spvC; P3, negative for agfA; and P4, negative for lpfA, spvC and sopE. The most prevalent profile was P1, which was present in 88% of the isolates. Although all isolates belong to the same serovar, it was possible to observe variations in the presence of these virulence-associated genes between different isolates. The characterization of the mechanisms of virulence circulating in the population of Salmonella Enteritidis is important for a better understanding of its biology and pathogenicity. The frequency of these genes and the establishment of genetic profiles can be used to determine patterns of virulence. These patterns, associated with in vivo studies, may help develop tools to predict the ability of virulence of different strains.

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Section: Discussionsupporting

confidence: 80%

“…The bacteria were retrieved from frozen culture stocks and cultured overnight at 37°C in brain heart infusion broth (Oxoid; Cambridge, United Kingdom). An aliquot of 1 mL of each bacterial culture was separated for DNA extraction by heat treatment as described by Borsoi et al (2009).…”

Section: Methodsmentioning

confidence: 99%

Detection of virulence-associated genes in Salmonella Enteritidis isolates from chicken in South of Brazil

et al. 2013

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“…A extração de DNA e o multiplex-PCR foram realizadas conforme Borsoi et al (11) . As amostras foram centrifugadas a 12.000 rpm por 10 minutos, o sobrenadante descartado e o pellet obtido suspenso em 800 µL de água ultrapura, homogeneizado e centrifugado a 12.000 rpm por cinco minutos.…”

Section: Methodsunclassified

Campylobacter jejuni e Campylobacter coli EM CARCAÇAS DE FRANGO RESFRIADAS E CONGELADAS

Cisco

Tedesco

Perdoncini³

et al. 2017

Ciênc. anim. bras.

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ResumoEspécies de Campylobacter spp. termotolerantes são agentes de surtos de campilobacteriose em humanos e os produtos de origem avícola são considerados uma importante fonte de infecção. Foram identificados Campylobacter jejuni e Campylobacter coli em carcaças de frango resfriadas e congeladas coletadas em três abatedouros entre 2014 e 2015. A detecção de Campylobacter spp. foi realizada por microbiologia convencional e a identificação de C. jejuni e C. coli por multiplex-PCR. Dentre as amostras avaliadas verificou-se Campylobacter spp. termotolerante em 63,8%, sendo 72,2% em carcaças resfriadas e 55,5% em carcaças congeladas. Destas, 83,3% foram positivas para C. jejuni e 66,6% para C. coli, enquanto 50% foram positivas para ambas as espécies. A presença de Campylobacter spp. termotolerante em carcaças de frangos de corte prontas para consumo representa uma importante fonte de transmissão destes patógenos para humanos. Palavras-chave: abatedouros avícolas; campilobacteriose; termotolerantes. AbstractSpecies of thermophilic Campylobacter spp. are agents of campylobacteriosis outbreaks in humans, and poultry products are implicated as major sources of infection. The detection of Campylobacter spp. was performed by conventional microbiology in poultry carcasses collected in slaughterhouses and species were identified by multiplex-PCR. Thermophilic Campylobacter spp. was verified in 63.8% of the samples, being 72.2% in chilled carcasses and 55.5% in frozen carcasses. Of these, 83.3% were positive for C. jejuni and 66.6% to C. coli, while 50% were positive for both. The presence of thermophilic Campylobacter spp. in ready-to-eat poultry products represents a potential source of human campylobacteriosis.

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“…Two characteristic colonies were submitted to biochemical tests and to agglutination reaction with somatic polyvalent serum to Salmonella spp. (Borsoi et al, 2009 …”

Section: Methodsmentioning

confidence: 99%

“…After reception and accommodation in each set, ten birds were randomly chosen in order to detect the antibodies titles to S. Enteritidis through the ELISA test (IDDEXX SE -Laboratories, Inc. Westbrook, USA) and to detect the presence of Salmonella spp. from conventional bacterial isolation (Borsoi et al, 2009). After having 2mL of blood collected through cardiac puncture, the chicks were euthanized through cervical disarticulation.…”

Section: Methodsmentioning

confidence: 99%

Establishment of a pathogenicity index in Salmonella Enteritidis and Salmonella Typhimurium strains inoculated in one-day-old broiler chicks

Lima

Furian

Pillati

et al. 2016

Arq. Bras. Med. Vet. Zootec.

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Salmonella Enteritidis and Salmonella Typhimurium are responsible for causing huge economic loses in aviculture, as they lead young broiler chicks to develop clinical disease and thus increase mortality. Salmonella's pathogenicity is considered complex and multifactorial, demanding more studies that could elucidate the interaction between host and pathogen. The present study aims to evaluate the virulence of 130S. Enteritidis isolates and 70S. Typhimurium inoculated in one-day-old chicks through the establishment of a pathogenicity index. For each strain, 10 commercial chicks from the Cobb lineage were used. Then, 200µL of a solution containing 2x10 8 CFU of S. Enteritidis or S. Typhimurium were inoculated in the birds by intraperitoneal via. Mortality and presence of lesions such as aerosaculitis (A), perihepatitis (Ph), pericarditis (Pc), peritonitis (Pt), onfalitis (O) and cellulitis (C) were registered daily for seven days. From the second to the seventh day there was a proportional decrease in the punctuation of the time of death (TD) for each day that the bird had survived. The pathogenicity index was calculated using the following formula: PI = (TD x 5) + A + Ph + Pc + Pt + O + C. The obtainment of the PI of each bacterial sample was achieved by calculating the rate of the ten inoculated birds. Based on the obtained results, it was possible to attribute the pathogenicity value for each strain, which enabled us to classify them in groups of low (27/200), intermediate (95/200) and high (78/200) pathogenicity. The utilization of standards like time of death and presence of septicemic lesions made it possible to determine the pathogenicity rate for each strain. Besides that, the proposed model has presented dramatic differences between the high, intermediate and low pathogenicity groups, which makes this mechanism useful for further classification of strains isolated in poultry farms.

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Inoculation of newly hatched broiler chicks with two Brazilian isolates of Salmonella Heidelberg strains with different virulence gene profiles, antimicrobial resistance, and pulsed field gel electrophoresis patterns to intestinal changes evaluation

Cited by 46 publications

References 30 publications

Detection of virulence-associated genes in Salmonella Enteritidis isolates from chicken in South of Brazil

Detection of virulence-associated genes in Salmonella Enteritidis isolates from chicken in South of Brazil

Campylobacter jejuni e Campylobacter coli EM CARCAÇAS DE FRANGO RESFRIADAS E CONGELADAS

Establishment of a pathogenicity index in Salmonella Enteritidis and Salmonella Typhimurium strains inoculated in one-day-old broiler chicks

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