Inoculation of in vitro cultures with rhizosphere microorganisms improve plant development and acclimatization during immature embryo rescue in nectarine and pear breeding programs

Cantabella, Daniel; Dolcet-Sanjuan, Ramón; Casanovas, María; Solsona, C.; Teixidó, Neus

doi:10.1016/j.scienta.2020.109643

Cited by 14 publications

(9 citation statements)

References 49 publications

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“…Previous studies have shown that the presence of P. oryzihabitans PGP01 induces modifications in Pyrus and Prunus rootstocks ( Cantabella et al , 2020 , 2021 ). The data presented in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…In plants, P. oryzihabitans has been linked to panicle blight in rice ( Hou et al , 2020 ) and to stem and leaf rot in muskmelon ( Li et al , 2021 ). However, other studies have shown that P. oryzihabitans PGP01 can exert a positive effect on root growth ( Belimov et al , 2015 ; Cantabella et al , 2020 ). The aims of the present study were firstly to determine the effects of P. oryzihabitans on RSA in A. thaliana , secondly to characterize how perception of P. oryzihabitans alters the root transcriptome profile, and thirdly to determine whether ROS-related mechanisms were involved in the responses of RSA to perception of the presence of the bacterium.…”

Section: Introductionmentioning

confidence: 98%

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Non-volatile signals and redox mechanisms are required for the responses of Arabidopsis roots toPseudomonas oryzihabitans

Cantabella

Karpińska

Teixidó

et al. 2022

Journal of Experimental Botany

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Soil bacteria promote plant growth and protect against environmental stresses but the mechanisms involved remain poorly characterised, particularly when there is no direct contact between the roots and bacteria. Here, we explored the effects of Pseudomonas oryzihabitans PGP01 on the root system architecture (RSA) in Arabidopsis thaliana seedlings. Significant increases in lateral root (LR) density were observed when seedlings were grown in the presence of P. oryzihabitans, as well as an increased abundance of transcripts associated with altered nutrient transport and phytohormone responses. However, no bacterial transcripts were detected on the root samples by RNAseq analysis, demonstrating that the bacteria do not colonise the roots. Separating the agar containing bacteria from the seedlings prevented the bacteria-induced changes in RSA. Bacteria-induced changes in RSA were absent from mutants defective in Ethylene Response Factor (ERF109), glutathione synthesis (pad2-1, cad2-1 and rax1-1) and in strigolactone (SL) synthesis (max3-9 and max4-1) or signalling (max2-3). However, the P. oryzihabitans-induced changes in RSA were similar in the low ascorbate mutants (vtc2-1, vtc2-2) to the wild type controls. Taken together, these results demonstrate the importance of non-volatile signals and redox mechanisms in the root architecture regulation that occurs following long-distance perception of P. oryzihabitans.

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“…Previous studies have shown that the presence of P. oryzihabitans PGP01 induces modifications in Pyrus and Prunus rootstocks ( Cantabella et al , 2020 , 2021 ). The data presented in Fig.…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Non-volatile signals and redox mechanisms are required for the responses of Arabidopsis roots toPseudomonas oryzihabitans

Cantabella

Karpińska

Teixidó

et al. 2022

Journal of Experimental Botany

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“…Contrarily, C. ramotenellum is mostly associated with plants but presents a dual nature. On one hand, the species has been described as a beneficial mycorrhizal fungus [ 101 , 102 ], but it has also as been the cause of ripening in grape berries and mandarins [ 103 , 104 ]. The presence of C. allicinum and C. ramotenellum might be associated with migrating birds, which arrive for the breeding period on the nearby coast (Wedel Jarlsberg Land) [ 105 ].…”

Section: Discussionmentioning

confidence: 99%

The First Comprehensive Biodiversity Study of Culturable Fungal Communities Inhabiting Cryoconite Holes in the Werenskiold Glacier on Spitsbergen (Svalbard Archipelago, Arctic)

Borzęcka

Suchodolski

Dudek

et al. 2022

Biology

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Cryoconite holes on glacier surfaces are a source of cold-adapted microorganisms, but little is known about their fungal inhabitants. Here, we provide the first report of distinctive fungal communities in cryoconite holes in the Werenskiold Glacier on Spitsbergen (Svalbard Archipelago, Arctic). Due to a combination of two incubation temperatures (7 °C and 24 ± 0.5 °C) and two media during isolation (PDA, YPG), as well as classical and molecular identification approaches, we were able to identify 23 different fungi (21 species and 2 unassigned species). Most of the fungi cultured from cryoconite sediment were ascomycetous filamentous micromycetes. However, four representatives of macromycetes were also identified (Bjerkandera adusta, Holwaya mucida, Orbiliaceae sp., and Trametes versicolor). Some of the described fungi possess biotechnological potential (Aspergillus pseudoglaucus, A. sydowii, Penicillium expansum, P. velutinum, B. adusta, and T. versicolor), thus, we propose the Arctic region as a source of new strains for industrial applications. In addition, two phytopathogenic representatives were present (P. sumatraense, Botrytis cinerea), as well as one potentially harmful to humans (Cladosporium cladosporioides). To the best of our knowledge, we are the first to report the occurrence of A. pseudoglaucus, C. allicinum, C. ramotenellum, P. sumatraense, P. velutinum, P. cumulodentata, B. adusta, and T. versicolor in polar regions. In all likelihood, two unassigned fungus species (Orbiliaceae and Dothideomycetes spp.) might also be newly described in such environments. Additionally, due to experimenting with 10 sampling sites located at different latitudes, we were able to conclude that the number of fungal spores decreases as one moves down the glacier. Considering the prevalence and endangerment of glacial environments worldwide, such findings suggest their potential as reservoirs of fungal diversity, which should not be overlooked.

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“…Previous studies have shown that the inoculation of nectarine (Prunus persica cv. Nectarina) embryos with P. oryzihabitans PGP01 induced root modifications that promote plant growth under greenhouse conditions (Cantabella et al 2020). The effects of bacteria belonging to Pseudomonas genus have been previously studied in A. thaliana .…”

Section: Discussionmentioning

confidence: 99%

Regulation of root architecture by Pseudomonas oryzihabitans is mediated by strigolactones and redox processes.

Cantabella

Karpińska

Teixidó

et al. 2020

Preprint

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Mechanisms that control of root system architecture are well characterised but little is known about how these processes respond to plant growth promoting rhizobacteria. We therefore studied how the presence of Pseudomonas oryzihabitans PGP01 altered wild type RSA and how these changes were modified in mutants that are defective antioxidant capacity (vtc2-1, vtc2-2, pad2-1, cad2-1 and rax1-1) or strigolactone (SL) synthesis (max3-9 and max4-1) or signalling (max2-3). The presence of P. oryzihabitans PGP01 decreased the length of primary and lateral roots but increased the number of lateral roots and lateral root density in the wild type roots but not in the SL mutants. The presence of synthetic SL, GR24 in combination with P. oryzihabitans PGP01 significantly decreased the number and length of lateral roots in the WT, max3-9 and max4-1 but not max2-3 seedlings. Lateral root density was increased in all genotypes in the presence of bacterium, but this effect was less pronounced in the ascorbate deficient vtc2-1 and vtc2-2 roots and absent from glutathione -deficient (pad2-1, cad2-1 and rax1-1) seedlings. Taken together, these results demonstrate the importance of SL-mediated signalling in root responses to growth promoting rhizobacteria, as well roles of cellular redox controls in these processes.

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Inoculation of in vitro cultures with rhizosphere microorganisms improve plant development and acclimatization during immature embryo rescue in nectarine and pear breeding programs

Cited by 14 publications

References 49 publications

Non-volatile signals and redox mechanisms are required for the responses of Arabidopsis roots toPseudomonas oryzihabitans

Non-volatile signals and redox mechanisms are required for the responses of Arabidopsis roots toPseudomonas oryzihabitans

The First Comprehensive Biodiversity Study of Culturable Fungal Communities Inhabiting Cryoconite Holes in the Werenskiold Glacier on Spitsbergen (Svalbard Archipelago, Arctic)

Regulation of root architecture by Pseudomonas oryzihabitans is mediated by strigolactones and redox processes.

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