Innovative mixture of salts in the quick, easy, cheap, effective, rugged, and safe method for the extraction of residual macrolides in milk followed by analysis with liquid chromatography and tandem mass spectrometry

Costa, Rafaela Pinto da; Spisso, Bernardete Ferraz; Pereira, Mararlene Ulberg; Monteiro, Mychelle Alves; Ferreira, Rosana Gomes; Nóbrega, Armi Wanderley da

doi:10.1002/jssc.201500373

Cited by 18 publications

(12 citation statements)

References 17 publications

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“…Promising extraction and pre-concentration techniques for veterinary drug residues that have been explored recently by many researchers include dispersive liquid-liquid microextraction (DLLME) [5,6,35,36], hollow fiber based liquidphase microextraction (HFLPME) [37][38][39][40] and quick, easy, cheap, effective, rugged and safe (QuEChERS) [4, [41][42][43] where the general trend is compliance with green chemistry principles. Veterinary drug residues occur at trace levels as low nanogram per gram [4,37] hence the need to pre-concentrate.…”

Section: Pre-concentration Techniquesmentioning

confidence: 99%

“…The quick easy cheap effective rugged safe (QuEChERS) method is an extraction technique that employs an organic solvent and phase separation using high salt content, in some cases followed by dispersive solid phase extraction (d-SPE) for sample clean up. The QuEChERS method, which was originally developed for pesticide analysis in fruits and vegetables [44,45], has recently been proposed for the analysis of veterinary drugs in different food matrices [4, 41,43,46]. Recent applications of this method are discussed below.…”

Section: Quechersmentioning

confidence: 99%

“…as donor phase and 10% (w/v) of Aliquat-336 in octanol as an SLM. da Costa et al [41] developed a modified QuEChERS extraction technique using acetonitrile, followed by the addition of a mixture of salts (sodium sulfate, sodium chloride and potassium carbonate) for the extraction of seven macrolide drugs in milk followed by analysis on liquid chromatography and tandem mass spectrometry (LC-MS/MS). Sodium sulfate and sodium chloride removed water from samples promoting the salting out effect while acetonitrile was used for deproteination.…”

Section: Quechersmentioning

confidence: 99%

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Modern Extraction and Cleanup Methods of Veterinary Drug Residues in Food Samples of Animal Origin

Moyo¹,

Tavengwa²

2019

Recent Advances in Analytical Chemistry

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Extensive research on the presence of veterinary drug residues in food samples has been conducted and is still underway. The inappropriate or excessive use of veterinary drugs in food producing animals may result in trace quantities of these drugs or their metabolites in food samples. Food contamination by veterinary drug residues is one of the main challenges worldwide to public health with drug resistance being the biggest threat. One of the challenges in veterinary drug residue analysis is their occurrence in trace amounts that are normally below limits of detection of most analytical instruments. Various efficient, economical, miniaturized and environmentally friendly extraction methods have been developed in recent years to pre-concentrate these analytes before instrumental analysis to enhance their detection and also to overcome the limitations of traditional extraction methods such as liquid-liquid extraction and solid phase extraction. These methods include quick, easy, cheap, effective, rugged and safe (QuEChERS), molecularly imprinted polymers, dispersive liquid-liquid microextraction and hollow fiber liquid-phase microextraction, and they will be discussed in this chapter.

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Section: Pre-concentration Techniquesmentioning

confidence: 99%

Section: Quechersmentioning

confidence: 99%

Section: Quechersmentioning

confidence: 99%

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Modern Extraction and Cleanup Methods of Veterinary Drug Residues in Food Samples of Animal Origin

Moyo¹,

Tavengwa²

2019

Recent Advances in Analytical Chemistry

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“…The most commonly used technique for the determination of macrolide antibiotics is liquid chromatography combined with coupled mass spectrometric detection [23]. The diode array detector is not so powerful due to the lack of sensitive chromophore(s) of these antibiotics, but chemical derivatization can increase its sensitivity [24].…”

Section: Introductionmentioning

confidence: 99%

Voltammetric behavior and determination of the macrolide antibiotics azithromycin, clarithromycin and roxithromycin at a renewable silver – amalgam film electrode

Vajdle

Guzsvány

Škorić

et al. 2017

Electrochimica Acta

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The renewable silver-amalgam film electrode (Hg(Ag)FE) was applied for voltammetric characterization and determination of semi-synthetic macrolide antibiotics azithromycin (AZI), clarithromycin (CLA) and roxithromycin (ROX) in the Britton-Robinson buffer as supporting electrolyte ranging the pH from 4.0 to 11.9. All three macrolides showed reduction signals in fairly negative potential range.During direct cathodic square wave voltammetric (SWV) investigations conducted over the potential range from À0.75 V to À2.00 V vs SCE, either one or two reduction peaks were obtained in the potential range from À1.5 to À1.9 V. The shapes and intensities of the signals depend on the applied pH values in wider pH ranges. For analytical purposes concerning the development of direct cathodic SWV and adsorptive stripping SWV (SW-AdSV) methods the neutral and slightly alkaline media were suitable as pH 7.2, pH 7.4 and pH 7.0 for AZI, CLA and ROX, respectively. Based on the cyclic voltammograms recorded at these pH values, adsorption-controlled electrode kinetics process can be proposed for all three macrolides. Furthermore, the water suppressed 1 H NMR measurements in the pH range between 6.0 and 10.5 indicated that the macrolide molecules at the optimal analytical conditions are predominantly in protonated form via their tertiary amino groups which supported in all three cases their adsorption on the appropriately polarized Hg(Ag)FE electrode. The optimized direct cathodic SWV methods showed good linearity in concentration ranges 4.81-23.3 mg mL À1 , 1.96-28.6 mg mL À1 and 1.48-25.9 mg mL À1 for AZI, CLA and ROX, respectively. The development of the SW-AdSV methods resulted in the linear responses at lower concentration ranges as 1.0-2.46 mg mL À1, 0.05-0.99 mg mL À1 and 0.10-0.99 mg mL À1 , for AZI, CLA and ROX, respectively. The relative standard deviation for all developed methods was not higher than 1.0% except the SWV method for AZI with 4.7%. In the case of all three investigated macrolide antibiotics the protonated form of the tertiary amino group(s) at appropriate accumulation potential and time favored the adsorption of the ionic form of the target molecules offering the opportunity for the development of SW-AdSV methods for their trace level analysis on Hg(Ag)FE. Optimized SW-AdSV method was applied for determination of ROX in pharmaceutical preparation Runac

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“…LC–MS/MS has been recognized as the most popular and practical technique for the determination of macrolide residues in food owing to its high selectivity and sensitivity . Nevertheless, basic macrolides suffer from peak tailing on traditional silica‐based RPLC column, which severely influences the column efficiency and thus the detection sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Separation analysis of macrolide antibiotics with good performance on a positively charged C18HCE column

Wei

Shen

Yan

et al. 2016

J of Separation Science

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The separation of basic macrolide antibiotics suffers from peak tailing and poor efficiency on traditional silica-based reversed-phase liquid chromatography columns. In this work, a C18HCE column with positively charged surface was applied to the separation of macrolides. Compared with an Acquity BEH C18 column, the C18HCE column exhibited superior performance in the aspect of peak shape and separation efficiency. The screening of mobile phase additives including formic acid, acetic acid and ammonium formate indicated that formic acid was preferable for providing symmetrical peak shapes. Moreover, the influence of formic acid content was investigated. Analysis speed and mass spectrometry compatibility were also taken into account when optimizing the separation conditions for liquid chromatography coupled with tandem mass spectrometry. The developed method was successfully utilized for the determination of macrolide residues in a honey sample. Azithromycin was chosen as the internal standard for the quantitation of spiramycin and tilmicosin, while roxithromycin was used for erythromycin, tylosin, clarithromycin, josamycin and acetylisovaleryltylosin. Good correlation coefficients (r(2) > 0.9938) for all macrolides were obtained. The intra-day and inter-day recoveries were 73.7-134.7% and 80.7-119.7% with relative standard deviations of 2.5-8.0% and 3.9-16.1%, respectively. Outstanding sensitivity with limits of quantitation (S/N ≥ 10) of 0.02-1 μg/kg and limits of detection (S/N ≥ 3) of 0.01-0.5 μg/kg were achieved.

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Innovative mixture of salts in the quick, easy, cheap, effective, rugged, and safe method for the extraction of residual macrolides in milk followed by analysis with liquid chromatography and tandem mass spectrometry

Cited by 18 publications

References 17 publications

Modern Extraction and Cleanup Methods of Veterinary Drug Residues in Food Samples of Animal Origin

Modern Extraction and Cleanup Methods of Veterinary Drug Residues in Food Samples of Animal Origin

Voltammetric behavior and determination of the macrolide antibiotics azithromycin, clarithromycin and roxithromycin at a renewable silver – amalgam film electrode

Separation analysis of macrolide antibiotics with good performance on a positively charged C18HCE column

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