Abstract:Although two types of nerve endings have been proposed to innervate blood vessels in the dental pulp, the precise innervation pattern is not well understood. This is mainly due to the lack of information regarding the positional relationships of nerve fibers with blood vessels at the electron microscopic level.The rat incisor pulp was investigated by scanning electron microscopy (SEM) after connective tissue digestion and by transmission electron microscopy after immunohistochemical localization with polyclona… Show more
“…The mouse incisor is innervated by sensory and sympathetic nerves (Ishizuka and Hiura, 1992; Johansson et al, 1992; Tabata et al, 1998; Zhang et al, 1998) but is devoid of parasympathetic nerves (Olgart, 1996; Sasano et al, 1995). Nerve fibers accompanying the arteries are located within the periarterial region, which is similar to the tunica adventitia of free arteries.…”
Mesenchymal stem cells (MSCs) are typically defined by their in vitro characteristics, and as a consequence the in vivo identity of MSCs and their niches are poorly understood. To address this issue, we used lineage tracing in a mouse incisor model and identified the neurovascular bundle (NVB) as an MSC niche. We found that NVB sensory nerves secrete Shh protein, which activates Gli1 expression in periarterial cells that contribute to all mesenchymal derivatives. These periarterial cells do not express classical MSC markers used to define MSCs in vitro. In contrast, NG2+ pericytes represent an MSC subpopulation derived from Gli1+ cells; they express classical MSC markers and contribute little to homeostasis but are actively involved in injury repair. Likewise, incisor Gli1+ cells but not NG2+ cells exhibit typical MSC characteristics in vitro. Collectively, we demonstrate that MSCs originate from periarterial cells and are regulated by Shh secretion from a NVB.
“…The mouse incisor is innervated by sensory and sympathetic nerves (Ishizuka and Hiura, 1992; Johansson et al, 1992; Tabata et al, 1998; Zhang et al, 1998) but is devoid of parasympathetic nerves (Olgart, 1996; Sasano et al, 1995). Nerve fibers accompanying the arteries are located within the periarterial region, which is similar to the tunica adventitia of free arteries.…”
Mesenchymal stem cells (MSCs) are typically defined by their in vitro characteristics, and as a consequence the in vivo identity of MSCs and their niches are poorly understood. To address this issue, we used lineage tracing in a mouse incisor model and identified the neurovascular bundle (NVB) as an MSC niche. We found that NVB sensory nerves secrete Shh protein, which activates Gli1 expression in periarterial cells that contribute to all mesenchymal derivatives. These periarterial cells do not express classical MSC markers used to define MSCs in vitro. In contrast, NG2+ pericytes represent an MSC subpopulation derived from Gli1+ cells; they express classical MSC markers and contribute little to homeostasis but are actively involved in injury repair. Likewise, incisor Gli1+ cells but not NG2+ cells exhibit typical MSC characteristics in vitro. Collectively, we demonstrate that MSCs originate from periarterial cells and are regulated by Shh secretion from a NVB.
“…The avidinâbiotin complex (ABC) visualization method was reported in our previous study (Tabata et al, 1998). Briefly, the circumvallate papillae were put into a solution containing 0.1% glutaraldehyde and 4% paraformaldehyde in a phosphate buffer for 4 h on ice.…”
The taste buds of bovine circumvallate papillae were investigated under light and electron microscopy both by histological and immunohistochemical methods. Taste buds existed in the inner epithelium of the trench of the papillae. Under electron microscopy, two types of taste cells, type I and type II, could be classified according to the existence of dense-cored vesicles and cytoplasmic density. Type I had electron-lucent cytoplasm and possessed many electron-dense cored vesicles in the apical cytoplasm. It was considered that the electron-dense materials of the vesicles were released and constituted the pore substance. This type of cell possessed long and thick apical processes in the taste pore. Type II had denser electron cytoplasm compared with that of type I and possessed many electron-lucent vesicles in the apical cytoplasm. This type of cell possessed microvilli in the taste pore. To know the immunoreactivity to âŁ-gustducin in bovine circumvallate taste buds, we used the immunoblotting method and the immunohistochemical method. The âŁ-gustducin reaction band at 40 kDa was displayed in the specimen of Western blots. The immunohistochemical property of the antiserum to âŁ-gustducin was investigated by using the avidin-biotin complex (ABC) method and the 1.4-nm gold and silver enhancement methods. A subset of taste cells showed the immunoreactivity under light microscopy. The electron microscopic specimens with the 1.4-nm gold and silver enhancement method revealed that only type II cells exhibited the âŁ-gustducin immunoreactivity. Anat Rec Part A 271A: 217-224, 2003.
“…Bacteria can invade dentinal tubules, but do not invade dentine because of the outward movement of dentinal fluid that contains immunoglobulins. 21 Thus, coronal dentine may have weak defences against bacteria and noxious agents penetrating into dentinal tubules. Previous studies reported that a higher rate of dentinal fluid flow may induce a larger stress on mechanoreceptors, resulting in an elevated intradental nerve firing rate.…”
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