Bovine circumvallate taste buds: Taste cell structure and immunoreactivity to α‐gustducin

Shoji, Tetsuo; Wada, Azumi; Kobayashi, Takahiko; Nishimura, Shigeyuki; Muguruma, Michio; Iwamoto, Hisao

doi:10.1002/ar.a.10028

Cited by 17 publications

(18 citation statements)

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“…Next, the sections were treated with 1% sodium borohydride for 2-3 min, incubated in phosphate-buffered saline (PBS) containing 1% bovine serum albumin, 1% goat serum and 0.05% Triton to block non-specific binding, and then incubated with an antiserum against gustducin (Santa Cruz Biotechnology Inc., Santa Cruz, CA) diluted in the blocking solution without Triton (1% bovine serum albumin and 1% goat serum in PBS) overnight at 4°C. The specificity of this antibody to bovine papillae was confirmed in our previous study [12]. Subsequently, the specimens were incubated with a biotinylated goat anti-rabbit secondary antibody (Vector Laboratories Inc., Burlingame, CA ; 1:1,000 dilution in the blocking solution without Triton) for 4 hr at 4°C, washed with PBS, and reacted with an avidin-biotin-horseradish peroxidase (HRP) conjugate (Vector Laboratories Inc., Burlingame, CA) overnight at 4°C.…”

Section: Methodssupporting

confidence: 76%

“…The avidin-biotin complex (ABC) visualization method was carried out as described previously [11,12]. Briefly, the fungiform papillae were fixed in a phosphate buffer containing 0.1% glutaraldehyde and 4% paraformaldehyde for 4 hr on ice, and then cut into 50 µm sections with a microslicer (Dosaka, Kyoto, Japan).…”

Section: Methodsmentioning

confidence: 99%

“…Our previous study of bovine circumvallate papillae demonstrated that type II taste cells exhibit immunoreactivity for a gustducin antiserum [12]. In the bovine tongue, two types of papillae, namely the circumvallate and fungiform papillae, contain taste buds.…”

mentioning

confidence: 99%

“…68(9): 953-957, 2006 Gustducin, a G-protein specific for taste cells [7], has been reported to be involved in not only bitter but also sweet taste transduction [5-7, 9, 14]. Our previous study of bovine circumvallate papillae demonstrated that type II taste cells exhibit immunoreactivity for a gustducin antiserum [12]. In the bovine tongue, two types of papillae, namely the circumvallate and fungiform papillae, contain taste buds.…”

mentioning

confidence: 99%

“…Semi-thin sections were cut with glass knives, stained with toluidine blue and observed with a Nikon Eclipse E 800 microscope. Ultra-thin sections were cut with a diamond knife, mounted on formvar-supported one-slot grids, stained with lead citrate and uranyl acetate and observed with a Hitachi H-600 transmission electron microscope.The avidin-biotin complex (ABC) visualization method was carried out as described previously [11,12]. Briefly, the fungiform papillae were fixed in a phosphate buffer containing 0.1% glutaraldehyde and 4% paraformaldehyde for 4 hr on ice, and then cut into 50 µm sections with a microslicer (Dosaka, Kyoto, Japan).…”

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confidence: 99%

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Structure of Bovine Fungiform Taste Buds and Their Immunoreactivity for Gustducin

Shoji

Kudo

Wada-Takemura

et al. 2006

The Journal of Veterinary Medical Science

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ABSTRACT. The taste buds of bovine fungiform papillae were studied by light and electron microscopy using both histological and immunohistochemical methods. The taste buds existed in the epithelium of the apical region of the papillae. By electron microscopy, two types of taste cells, namely type I and type II cells, could be classified according to the presence of dense-cored vesicles, the cytoplasmic density and the cell shape. Type I cells were thin, had an electron-dense cytoplasm containing dense-cored vesicles, and possessed long thick apical processes in the taste pore. Type II cells were thick, had an electron-lucent cytoplasm containing many electron-lucent vesicles, rather than dense-cored vesicles, and possessed microvilli in the taste pore. Immunohistochemical staining with an antiserum against gustducin was investigated by both light and electron microscopy using the avidin-biotin complex (ABC) method. Some, but not all, of the type II cells exhibited gustducin immunoreactivity, whereas none of the type I cells showed any immunoreactivity. KEY WORDS: bovine, fungiform papilla, gustducin, structure, taste bud.J. Vet. Med. Sci. 68(9): 953-957, 2006 Gustducin, a G-protein specific for taste cells [7], has been reported to be involved in not only bitter but also sweet taste transduction [5-7, 9, 14]. Our previous study of bovine circumvallate papillae demonstrated that type II taste cells exhibit immunoreactivity for a gustducin antiserum [12]. In the bovine tongue, two types of papillae, namely the circumvallate and fungiform papillae, contain taste buds. The present study investigated the structures of the taste cells in bovine fungiform papillae and their immunoreactivities for the gustducin antiserum. MATERIALS AND METHODSAfter collecting bovine tongues from a local slaughterhouse, the fungiform papillae were removed and fixed with 2% glutaraldehyde and 2% paraformaldehyde in 0.1 M phosphate buffer (pH 7.4) for the histological study or 0.1% glutaraldehyde and 4% paraformaldehyde for the immunocytological study. For the histological study, the fungiform papillae were placed into the above-mentioned fixatives for 4 hr on ice. The specimens were then washed in the phosphate buffer overnight, and post-fixed in 1% OsO 4 in the same phosphate buffer for 1 hr on ice. After dehydration in an ascending series of ethanol and absolute acetone, the specimens were embedded in epoxy resin. Semi-thin sections were cut with glass knives, stained with toluidine blue and observed with a Nikon Eclipse E 800 microscope. Ultra-thin sections were cut with a diamond knife, mounted on formvar-supported one-slot grids, stained with lead citrate and uranyl acetate and observed with a Hitachi H-600 transmission electron microscope.The avidin-biotin complex (ABC) visualization method was carried out as described previously [11,12]. Briefly, the fungiform papillae were fixed in a phosphate buffer containing 0.1% glutaraldehyde and 4% paraformaldehyde for 4 hr on ice, and then cut into 50 µm sections with a microslicer (Dosa...

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Section: Methodssupporting

confidence: 76%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Structure of Bovine Fungiform Taste Buds and Their Immunoreactivity for Gustducin

Shoji

Kudo

Wada-Takemura

et al. 2006

The Journal of Veterinary Medical Science

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Morphological and physiological aspects of digestive processes in the graminivorous primate Theropithecus gelada—a preliminary study

Mau

Johann

Sliwa³

et al. 2010

American J Primatol

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Hindgut fermentation has been suggested to contribute significantly to the digestive process in the gelada (Theropithecus gelada). We therefore hypothesized that in an in vitro fermentation test (Hohenheim gas test, using gas production as measure of microbial digestion) inoculum based on fresh gelada feces would degrade grass to a similar degree as zebra (Equus burchelli chapmani) feces and to a higher degree than that of hamadryas baboons (Papio hamadryas). Additionally, morphology of gelada tongue, salivary glands, stomach, and intestine were examined in this study. Gas production was measured between 4 and 96 hr using animal feces incubated with 200 mg of air-dry hay or mixed concentrate sample. For grass hay, 12-hr gas production was as follows: T. gelada (19.9 ml)>Papio (18.4 ml)>Equus (15.7 ml). After 24 hr, gas production changed: Papio (35.1 ml)>T. gelada (31.9 ml)>Equus (27.9 ml). At 96 hr, Papio was unexpectedly the most effective species with the highest gas production (53.1 ml)>zebra (51.2 ml)>gelada (49.4 ml). With a concentrate standard, 12-hr gas production was as follows: T. gelada (38.5 ml)>Equus (36.8 ml) = Papio (36.4 ml). At 24 hr, gas production differed: Papio (51.7 ml)>Equus (47.0 ml) = T. gelada (46.8 ml). At 96 hr, zebra was the most effective species with the highest gas production (63.9 ml)>Papio (60 ml) = T. gelada (59.9 ml). In conclusion, the results show that the microbial population present in gelada feces is able to ferment forage and concentrate substrates in vitro, although this fermentation did not occur with the expected effectiveness. Future studies should therefore focus also on the bacteria species involved.

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Distinct expression pattern of insulin‐like growth factor family in rodent taste buds

Takeda

Sakakura

Suzuki

2004

J of Comparative Neurology

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The insulin-like growth factor (IGF) system is an important regulator of growth and differentiation in a variety of tissues. In the present study, the expression of IGF family members in the taste buds of mice and rats was examined. By reverse transcriptase polymerase chain reaction (RT-PCR) analysis, mRNA of IGF-I and -II, IGF-I receptor (IGF-IR), insulin receptor (insulin R), and IGF-binding protein (IGFBP)-2, -3, -4, -5, and -6 was detected in the taste bud-containing epithelium of the circumvallate papillae of mice. As suggested by the study using degenerate PCR (McLaughlin [2000] J. Neurosci. 20:5679-5688), IGF-IR was expressed in most of the taste bud cells of adult mice, as found by immunohistochemistry, and in those of postnatal day (P) 6 mice by in situ hybridization. Insulin R, which has strong homology to IGF-IR, was also detected in most of the taste bud cells of mice by immunohistochemistry and in situ hybridization. IGF-I immunoreactivity was detected in a few taste bud cells and in the epithelium surrounding taste buds. Northern blot analysis revealed that the amount of IGF-I mRNA in taste bud-containing epithelium was very low compared with that in liver. IGF-II immunoreactivity was weakly detected in mouse taste buds and the surrounding epithelium. In the rat tissue, a subset of the taste bud cells was positive for IGF-II. Among the six IGFBPs, IGFBP-2, -5, and -6 were detected in the mouse taste buds: IGFBP-2 and -5 immunoreactivity was seen in the majority of the taste bud cells, whereas IGFBP-6 immunoreactivity was found in the nerve fibers innervating the taste buds. In situ hybridization study also revealed that IGFBP-2 and -5 mRNA was synthesized in the taste buds of P6 mice and that the expression of these mRNAs overlapped in von Ebner's glands. These data reveal that IGF-I and -II might be produced in taste bud cells and (or) surrounding lingual epithelium and act through IGF-IR and insulin R locally in a paracrine and autocrine manner. The activity of these IGFs may be modulated through their interaction with IGFBP-2, -5, and 6.

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Bovine circumvallate taste buds: Taste cell structure and immunoreactivity to α‐gustducin

Cited by 17 publications

References 50 publications

Structure of Bovine Fungiform Taste Buds and Their Immunoreactivity for Gustducin

Structure of Bovine Fungiform Taste Buds and Their Immunoreactivity for Gustducin

Morphological and physiological aspects of digestive processes in the graminivorous primate Theropithecus gelada—a preliminary study

Distinct expression pattern of insulin‐like growth factor family in rodent taste buds

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