Innate immunity of finfish: Primordial conservation and function of viral RNA sensors in teleosts

Aoki, Takashi; Hikima, Jun-ichi; Hwang, Seong Don; Jung, Taehun

doi:10.1016/j.fsi.2013.02.005

Cited by 83 publications

(77 citation statements)

References 168 publications

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“…1). In a similar manner, mda5 and lgp2 genes have been identified in all teleost fish studied so far, although the presence of rig1 is limited to ancient fish and has never been identified in modern fish (class Acanthopterygii) (Aoki et al, 2013), in which our fish species are included. Our data showed that the expression level of mda5 was upregulated in the SAF-1 cell line, which supports VNNV replication (Bandín et al, 2006), in a similar way to the zebrafish ZF-4 cell line, which also supports VNNV replication, in which rig1, mda5 and lgp2 transcription was upregulated by VNNV infection (Chen et al, 2015).…”

Section: Discussionmentioning

confidence: 65%

“…However, whilst mx gene expression was greatly induced, pkr transcription LGP2, Laboratory of Genetics and Physiology 2 protein; MAVS, mitochondrial antiviral signalling protein; TRAF3,, tumour necrosis factor (TNF) receptor-associated factor 3; TANK, TRAF family member-associated NFkB activator; TBK1, TANK-binding kinase 1; IRF3/7, IFN regulatory factor 3/7; Mx, myxovirus (influenza) resistance protein; PKR, dsRNA-dependent protein kinase receptor; ISRE, IFN-stimulated response element; ISG, IFN-stimulated gene. This figure contains the molecules found and analysed in this study, and is inspired by the literature (Aoki et al, 2013;Hansen et al, 2011;Takeuchi & Akira, 2008;Verrier et al, 2011;Zhang et al, 2014).…”

Section: Genes Of the Ifn Pathway Are Constitutively Expressedmentioning

confidence: 99%

“…Their activation in cells produces an antiviral state through the induction of many IFN-stimulated genes (ISGs), including genes such as that for the antiviral molecule myxovirus (influenza) resistance protein (Mx), with direct antiviral activity (Verrier et al, 2011). Thus, most of the studies in fish use the expression of mx genes as an indicator of viral infection and activation of the type I IFN response; the cellular components sensing the viral genomes and leading to the IFN response have already been characterized (Aoki et al, 2013;Zou et al, 2009). …”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Characterization of the IFN pathway in the teleost fish gonad against vertically transmitted viral nervous necrosis virus

Valero

Morcillo

Meseguer

et al. 2015

Journal of General Virology

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One of the most powerful innate immune responses against viruses is mediated by type I IFN. In teleost fish, it is known that virus infection triggers the expression of ifn and many IFN-stimulated genes, but the viral RNA sensors and mediators leading to IFN production are scarcely known. Thus, we have searched for the presence of these genes in gilt-head sea bream (Sparus aurata) and European sea bass (Dicentrarchus labrax), and evaluated their expression after infection with viral nervous necrosis virus (VNNV) in the brain, the main viral target tissue, and the gonad, used to transmit the virus vertically. In sea bream, a fish species resistant to the VNNV strain used, we found an upregulation of the genes encoding MDA5 (melanoma differentiation-associated gene 5), TBK1 (TANK-binding kinase 1), IRF3 (IFN regulatory factor 3), IFN, Mx [myxovirus (influenza) resistance protein] and PKR (dsRNA-dependent protein kinase receptor) proteins in the brain, which were unaltered in the gonad and could favour the dissemination by gonad fluids or gametes. Strikingly, in European sea bass, a very susceptible species, we also identified, transcripts coding for LGP2 (Laboratory of Genetics and Physiology 2), MAVS (mitochondrial antiviral signalling), TRAF3 (TNF receptor-associated factor 3), TANK (TRAF family member-associated NFkB activator) and IRF7 (IFN regulatory factor 7), and found that all the genes analysed were upregulated in the gonad, but only mda5, lgp2, irf3, mx and pkr were upregulated in the brain. These findings supported the notion that the European sea bass brain innate immune response is unable to clear the virus and pointed to the importance of gonad immunity to control the dissemination of VNNV to the progeny -an aspect that is worth investigating in aquatic animals.

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Section: Discussionmentioning

confidence: 65%

Section: Genes Of the Ifn Pathway Are Constitutively Expressedmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of the IFN pathway in the teleost fish gonad against vertically transmitted viral nervous necrosis virus

Valero

Morcillo

Meseguer

et al. 2015

Journal of General Virology

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show abstract

“…Chicken TLR21 was suggested to be a CpG ODN receptor [64]. As the TLR21 genes of teleosts and chicken are similar, teleost TLR21 is also considered to be a CpG motif receptor [63,65]. In fact, zebrafish Danio rerio TLR21 responded preferentially to CpG-ODN with GTCGTT motifs to exhibit antimicrobial activity [66].…”

Section: Nucleic Acid-sensing Tlrs In Teleostsmentioning

confidence: 99%

“…Teleost and mammalian RLRs have a number of structural similarities [65]. In teleosts, the RIG-I, MDA5 and…”

Section: Retinoic Acid Inducible Gene (Rig)-i-like Receptors (Rlrs) Imentioning

confidence: 99%

DNA vaccine-mediated innate immune response triggered by PRRs in teleosts

2015

Self Cite

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Osteichthyes: Immune Systems of Teleosts (Actinopterygii)

Nakanishi

Hikima

Yada

2018

Advances in Comparative Immunology

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Innate immunity of finfish: Primordial conservation and function of viral RNA sensors in teleosts

Cited by 83 publications

References 168 publications

Characterization of the IFN pathway in the teleost fish gonad against vertically transmitted viral nervous necrosis virus

Characterization of the IFN pathway in the teleost fish gonad against vertically transmitted viral nervous necrosis virus

DNA vaccine-mediated innate immune response triggered by PRRs in teleosts

Osteichthyes: Immune Systems of Teleosts (Actinopterygii)

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