Innate Immune Responses to Wildtype and Attenuated Sheeppox Virus Mediated Through RIG-1 Sensing in PBMC In-Vitro

Rufael, Tesfaye; Kangethe, Richard T.; Berguido, Francisco J.; Settypalli, Tirumala Bharani Kumar; Liu, Yang; Grabherr, Reingard; Loitsch, Angelika; Sassu, Elena L.; Pichler, Rudolf; Cattoli, Giovanni; Diallo, Adama; Wijewardana, Viskam; Lamien, Charles Euloge

doi:10.3389/fimmu.2021.666543

Cited by 5 publications

(2 citation statements)

References 35 publications

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“…5A-C). Additionally, retinoic acid inducible gene 1 (Rig-1) has been con rmed to mediate impaired cell death and immune responses [35][36][37]. Our results showed that compared to the sham group, the AAC group showed a signi cant reduction in RIG-1 expression.…”

Section: Resultsmentioning

confidence: 75%

Pinoresinol diglucoside alleviates pressure overload-induced cardiac injury via the AMPK/SIRT3/RIG-1 pathway

Xie

Sui

Qin

et al. 2022

Preprint

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Aims Heart failure (HF) often results from mechanical or neurohormonal stress and remains the mortality up to 45–50%, which is accompanied by cardiomyocytes loss, apoptosis, and fibrosis. Pinoresinol diglucoside (PDG), a compound obtained from with Forsythia suspensa, Epicommia ulmoides and Styrax sp., has a remarkable effect on anti-hypertension, anti-inflammation and anti-oxidative stress. However, whether PDG protects against pressure overload induced cardiomyocytes fibrosis, apoptosis and oxidative stress has not been illustrated. This study aimed to determine the cardioprotective effects and the underlying mechanism of PDG on cardiomyocytes injury in pressure overload-induced rats. Methods Abdominal aortic constriction (AAC)-surgery was performed to mimic pressure overload-induced cardiac injury (fibrosis, apoptosis, oxidative stress, and inflammation) in rats. The collagen content deposit evaluation of heart in rats were evaluated by PSR staining. Furthermore, phenylephrine (PE) was used to make in vitro cell models of cardiac injury. We used quantitative real-time PCR (qRT-PCR) to test the expression of targeting mRNAs both in vivo and vitro. Western blot assay was used to detect the protein expressions of the AMPK/SIRT3/RIG-1 signaling pathway. Results AAC induced the significant cardiac injury in hearts of rats, as indicators of myocytes fibrosis, apoptosis, oxidative stress and inflammation, whereas PDG treatment reversed these pathological changes compared to AAC rats. Moreover, AAC-induced increased left ventricular (LV) fibrosis, apoptosis, oxidative stress and inflammation compared to the Sham mice, but these increases were significantly reduced by PDG treatment. Interestingly, PDG in AAC-induced rats decreased the expressions of p-AMPK and SIRT3 at the protein levels. These results of this cardio-protection are likely through targeting cardiomyocytes fibrosis, apoptosis, oxidative stress and inflammation, possibly mediated by AMPK and SIRT3. In addition, PDG also blocked PE-induced the fibrotic changes in cardiomyocytes. Thus, PDG may be a potential therapeutic agent in targeting pathological cardiac injury by inhibiting myocytes fibrosis, apoptosis, oxidative stress and inflammation in pressure overload-induced cardiac injury. Conclusions PDG significantly decreased AAC-induced cardiomyocytes fibrosis, apoptosis, oxidative stress and inflammation in rats through the AMPK/SIRT3/RIG-1 signaling pathway. These novel findings provide the evidence that PDG may be a promising therapeutic strategy for pathological cardiac remodeling and HF.

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Section: Resultsmentioning

confidence: 75%

Pinoresinol diglucoside alleviates pressure overload-induced cardiac injury via the AMPK/SIRT3/RIG-1 pathway

Xie

Sui

Qin

et al. 2022

Preprint

View full text Add to dashboard Cite

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“…However, whether either of these viruses completed their replication cycle in PBMCs to produce infectious progeny was not shown (Chibssa et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Primary bovine white blood cells support dissemination of Lumpy Skin Disease Virus while suppressing viral replication

Kumar,

Frid,

Sol

et al. 2024

Preprint

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Lumpy skin disease (LSD) is a severe infectious, emerging transboundary disease of cattle, caused by a Pox family DNA virus. Lumpy skin disease virus (LSDV) infection is associated with a febrile response followed by emergence of widespread dermal nodules. In addition to the skin, LSDV resides in multiple internal organs and can be isolated from the blood of infected cattle. LSDV is suggested to be mechanically transmitted by biting arthropods. Live attenuated vaccines are commonly used to control disease and its spread. We have characterized the tropism, replication, and dissemination of a LSDV field isolate and of an attenuated vaccine strain usingin vitrosystems. To follow virus infection and dissemination in living cells, we have generated recombinant viruses expressing green fluorescent protein (GFP) under a synthetic viral promoter. Recombinant, GFP-expressing, LSDVs demonstrated similar replication kinetics to their corresponding parental LSDV strains in a bovine kidney cell line (MDBK). We further demonstrated that LSDV-GFP productively replicated in a bovine macrophage cell line and in primary bovine foreskin cells with no apparent differences between the field isolate and the vaccine strain. When bovine peripheral blood mononuclear cells (PBMCs) were infected with either LSDV recombinant strain, we observed specific viral driven GFP fluorescence as well as significant viral gene expression. However, infected PBMCs failed to support substantial viral DNA replication and release of infectious progeny. Subsequent analysis of the anti-viral response revealed that heat treated (HT) LSDV induced the expression of interferon- stimulated genes (ISGs) in PBMCs, but this response was suppressed by infectious viruses. Finally, we show that despite failed replication, LSDV infected PBMCs transmitted the virus to recipient co-cultured MDBK cells and produced infectious foci, suggesting a potential role of PBMCs in LSDV dissemination.HighlightsVirulent and attenuated LSDV productively replicated in bovine kidney and bovine macrophage cell lines as well as in primary fibroblasts.Adherent white blood cells were susceptible to LSDV field and attenuated vaccine infection.LSDV showed active viral transcription in PBMCs yet no significant viral genome replication or production of infectious progeny.PBMCs infected with heat-treated LSDV but not with fully infectious viruses upregulated ISGs’ RNA.PBMCs transmitted and disseminated LSDV to contacting permissive cells.

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Cytokine profile in lambs naturally infected with sheeppox virus

Karakurt,

Coskun,

Beytut

et al. 2023

Trop Anim Health Prod

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Innate Immune Responses to Wildtype and Attenuated Sheeppox Virus Mediated Through RIG-1 Sensing in PBMC In-Vitro

Cited by 5 publications

References 35 publications

Pinoresinol diglucoside alleviates pressure overload-induced cardiac injury via the AMPK/SIRT3/RIG-1 pathway

Pinoresinol diglucoside alleviates pressure overload-induced cardiac injury via the AMPK/SIRT3/RIG-1 pathway

Primary bovine white blood cells support dissemination of Lumpy Skin Disease Virus while suppressing viral replication

Cytokine profile in lambs naturally infected with sheeppox virus

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