Innate Immune Responses to Replication of Porcine Reproductive And Respiratory Syndrome Virus in Isolated Swine Alveolar Macrophages

Aït-Ali, Tahar; Wilson, Alastair; Westcott, David G.; Clapperton, M.; Waterfall, Martin; Mellencamp, Martha A.; Drew, Trevor W.; Bishop, Stephen; Archibald, Alan

doi:10.1089/vim.2006.0078

Cited by 82 publications

(65 citation statements)

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“…PRRSV replicates in porcine macrophages and induces several cytokines including IL-6, IL-8, IL-10, and TNF-a involving pulmonary inflammation response [16,17]. NF-jB is a critical transcription factor regulating the transcription of many proinflammatory molecules that are thought to be important in the generation of inflammation, including certain adhesion molecules (ICAM-1), most cytokines (TNF-a, IL-6), and chemokines (IL-8) [18].…”

Section: Discussionmentioning

confidence: 99%

Activation of NF-κB by nucleocapsid protein of the porcine reproductive and respiratory syndrome virus

et al. 2010

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Nuclear factor kappa B (NF-κB) is a critical transcription factor in innate and adaptive immune response as well as cell proliferation and survival. Previous studies have demonstrated that porcine reproductive and respiratory syndrome virus (PRRSV) infection activated NF-κB pathways through IκB degradation in MARC-145 cells and alveolar macrophages. To evaluate the mechanisms behind this, we investigated the role of PRRSV structural proteins in the regulation of NF-κB. In this study, we screened the structural proteins of PRRSV by NF-κB DNA-binding assay and luciferase activity assay and demonstrated that PRRSV nucleocapsid (N) protein could activate NF-κB in MARC-145 cells. Furthermore, we revealed that the region between aa 30 and 73 of N protein was essential for its function in the activation of NF-κB. These results presented here provide a basis for understanding molecular mechanism of PRRSV infection and inflammation response.

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Section: Discussionmentioning

confidence: 99%

Activation of NF-κB by nucleocapsid protein of the porcine reproductive and respiratory syndrome virus

et al. 2010

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“…Previous studies have indicated that there is significant interbreed variation in monocyte function in pigs that could be important in innate immunity (22). Accordingly, we sampled PBMCs from at least two individual animals from six different breeds.…”

Section: Identification Of Monocyte Subpopulations In the Pigmentioning

confidence: 99%

Comparative Analysis of Monocyte Subsets in the Pig

Fairbairn

Kapétanovic

Beraldi

et al. 2013

The Journal of Immunology

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Human and mouse monocyte can be divided into two different subpopulations based on surface marker expression: CD14/16 and Ly6C/CX3CR1, respectively. Monocyte subpopulations in the pig were identified based on reciprocal expression of CD14 and the scavenger receptor CD163. The two populations, CD14hi-CD163low and CD14low-CD163hi, show approximately equal abundance in the steady-state. Culture of pig PBMCs in CSF1 indicates that the two populations are a maturation series controlled by this growth factor. Gene expression in pig monocyte subpopulations was profiled using the newly developed and annotated pig whole genome snowball microarray. Previous studies have suggested a functional equivalence between human and mouse subsets, but certain genes such as CD36, CLEC4E, or TREM-1 showed human-specific expression. The same genes were expressed selectively in pig monocyte subsets. However, the profiles suggest that the pig CD14low-CD163high cells are actually equivalent to intermediate human monocytes, and there is no CD14− CD16+ “nonclassical” population. The results are discussed in terms of the relevance of the pig as a model for understanding human monocyte function.

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“…Methods and models for this analysis are described by Detilleux (2011). Studies by Doeschl-Wilson et al (2009), Ait-Ali et al (2007, Vincent et al (2006), Opriessnig et al (2006) and Halbur et al (1998) suggest differences in host genetic response with respect to porcine reproductive and respiratory syndrome and postweaning multisystemic wasting syndrome in view of breeding for improved resistance.…”

Section: New Phenotypesmentioning

confidence: 99%

New phenotypes for new breeding goals in pigs

Merks¹,

Mathur²,

Knol³

2012

Animal

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Pig breeders in the past have adopted their breeding goals according to the needs of the producers, processors and consumers and have made remarkable genetic improvements in the traits of interest. However, it is becoming more and more challenging to meet the market needs and expectations of consumers and in general of the citizens. In view of the current and future trends, the breeding goals have to include several additional traits and new phenotypes. These phenotypes include (a) vitality from birth to slaughter, (b) uniformity at different levels of production, (c) robustness, (d) welfare and health and (e) phenotypes to reduce carbon footprint. Advancements in management, genomics, statistical models and other technologies provide opportunities for recording these phenotypes. These new developments also provide opportunities for making effective use of the new phenotypes for faster genetic improvement to meet the newly adapted breeding goals.

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Innate Immune Responses to Replication of Porcine Reproductive And Respiratory Syndrome Virus in Isolated Swine Alveolar Macrophages

Cited by 82 publications

References 62 publications

Activation of NF-κB by nucleocapsid protein of the porcine reproductive and respiratory syndrome virus

Activation of NF-κB by nucleocapsid protein of the porcine reproductive and respiratory syndrome virus

Comparative Analysis of Monocyte Subsets in the Pig

New phenotypes for new breeding goals in pigs

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