“…For this purpose the SI barrier has to be broken . This can be done by mutilation or removal of the stigmata (see the review by LINSKENS and KROH, 1967), changing the nutritional conditions (STOUT, 1931), pollen transplantation (KROH, 1966), chemical treatments (TATEBE, 1968), treatment with CO 2 (NAKANISHII et al ., 1969), high temperature (GONAI and HINATA, 1971) and bud pollination . The latter method is widely used for hybrid seed production, but is very time-consuming and demands skilled labour for maximal yield (JOHNSON et al ., 1970) .…”