Pollen germination and pollen tube growth following self-pollination and intra- and interspecific pollination of Medicago species

Sangduen, Nitsri; Sorensen, E. L.; Liang, G. H.

doi:10.1007/bf00021465

Cited by 10 publications

(2 citation statements)

References 14 publications

(10 reference statements)

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“…The S-RNase gene is a female determinant of self-incompatibility in other Solanaceae plants. S-RNase can degrade homologous pollen tube RNA, thereby inhibiting pollen tube growth and resulting in self-incompatibility [43]. This study also determined the S-RNase genotypes of the parents and extreme-phenotype offspring, and the results indicated that there were two distinct S-RNase alleles in each parent and F1 population offspring.…”

Section: Discussionmentioning

confidence: 85%

Localization of S-Locus-Related Self-Incompatibility in Lycium barbarum Based on BSA Analysis

Wang,

Wu,

Gao

et al. 2024

Horticulturae

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The recognition of pollen and pistil in the self-incompatibility process is generally determined by the interaction between the pollen S gene and pistil S gene located at the S locus. However, the regulatory mechanism of self-incompatibility in goji remains unknown. In this study, we used the self-compatible strain ‘13–19’ and self-incompatible strain ‘xin9’ from Ningxia as parents to create an F1 hybrid population. Reciprocal cross-pollination was performed within the same plant to evaluate the self-compatibility of the parents and F1 progeny. The parents and progeny were subjected to whole-genome resequencing, and mixed pools of DNA were constructed using 30 self-compatible and 30 self-incompatible individuals. Association analysis using the SNP-index method and Euclidean distance was employed to identify the key candidate region of the S locus. The candidate region was further annotated using the Swiss-Prot database to identify genes within the region. Additionally, transcriptome sequencing data from different organs/tissues, as well as from pistils of self-compatible and self-incompatible strains at control (0 h), short (0.5 h), medium (8 h), and long (48 h) time points after self-pollination and cross-pollination, were analyzed to assess differential gene expression and screen for self-compatibility-related loci. Specific primers were designed for PCR amplification to determine the S-RNase genotypes of the extreme parents. The results revealed that the S locus in goji is located within a 32.2 Mb region on chromosome 2 that contains a total of 108 annotated genes. Differential expression analysis showed that ten genes, including Lba02g01064, were specifically expressed in stamens, with four of them annotated as F-box genes, potentially serving as determinants of self-compatibility in stamens. Lba02g01102 was exclusively expressed in pistils and annotated as an S-RNase gene, likely involved in self-compatibility. The expression of Lba02g01102 in pistils decreased after self-pollination and cross-pollination. Six candidate genes exhibited significant changes after self-pollination and cross-pollination. Both parents and progeny carried two S-RNase alleles, and the S-RNase genotypes showed a significant correlation with self-compatibility, with the self-compatible progeny containing the S8-RNase allele. The identification of the S locus in goji provides molecular markers for future marker-assisted breeding and offers genetic resources for studying the mechanism of self-incompatibility in goji, thus contributing to the improvement of goji varieties.

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Section: Discussionmentioning

confidence: 85%

Localization of S-Locus-Related Self-Incompatibility in Lycium barbarum Based on BSA Analysis

Wang,

Wu,

Gao

et al. 2024

Horticulturae

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“…Sangduen and co-workers [20] studied the pollen tube growth of M. scutellata in male sterile M. sativa styles and noted that few pollen grains penetrated the style. Abnormal pollen tube-style interactions occurred and fertilization was very infrequent.…”

Section: Introductionmentioning

confidence: 99%

Embryo culture of Medicago scutellata and M. sativa

Bauchan

1987

Plant Cell Tiss Organ Cult

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Resistance to the alfalfa weevil (Hypera postica (Gyllenhal)) and the potato leafhopper (Empoasco fabae (Harris)) is lacking in cultivated alfalfa. However, a closely related annual Medicago, Medicago scutellata¢ possesses dense glandular stem and leaf hairswhich provides a mechanism for resistance. Several attempts have been made at transfering the glandular haired trait from M. seutellata to perennial alfalfa with limited success. Earlier studies have shown that one reason for the lack of success is embryo abortion. Therefore, this study was initiated to observe zygotic embryo-genesis and to develop an embryo rescue technique for M. scutellata and M. sativa. Observations of zygotic embryogenesis showed that the two species are similar in morphology and can be described from youngest to oldest as globular, heart, torpedo, and hook shaped embryos. M. sativa embryos are smaller than M. scutellata embryos and develop three to four days later. Self pollinated M. scutellata (PI 307446) and sib mated M. sativa (Saranac AR) embryos were cultivated on Murashige and Skoog (MS) basal medium with various combination of 2,4-dichlorophenoxyacetic acid (2,4-D), indolacetic acid (IAA), 6-benzylaminopurine (BAP), and kinetic (KIN). Embryos from both species were also cultured on Schenk and Hildebrandt's (SH) basal medium with the addition of L-glutamine and L-proline. The experimental design was a completely randomized factorial for each experiment. Heart and torpedo shaped embryos from M. scutellata grew best (27.5% plantlet recovery) when cultured on MS medium with 0.05 mgl -t of both IAA and BAP. After 15 to 30 days on this medium, the embryos had only developed shoots. Therefore, it was necessary to transfer the shoots to MS basal medium without phytohormones for rooting. Rooting occurred in 15 to 30 days and the plantlets could be acclimatized to soil within 2 to 4 weeks. M. sativa embryos grew best (31% plantlet recovery~ on SH medium with 50 mM L-glutamine. M. sativa embryos developed both shoots and roots on this medium. This information may now be applied to the development of an embryo culture method for recovering insect resistant hybrids between M. scutellata and M. sativa.

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Pollen grain germination and pollen tube growth following in vivo and in vitro self and interspecific pollinations in annual Cicer species

1987

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Pollen germination and pollen tube growth following self-pollination and intra- and interspecific pollination of Medicago species

Cited by 10 publications

References 14 publications

Localization of S-Locus-Related Self-Incompatibility in Lycium barbarum Based on BSA Analysis

Localization of S-Locus-Related Self-Incompatibility in Lycium barbarum Based on BSA Analysis

Embryo culture of Medicago scutellata and M. sativa

Pollen grain germination and pollen tube growth following in vivo and in vitro self and interspecific pollinations in annual Cicer species

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