Inkjet printing for high-throughput cell patterning

Roth, Elisabeth. A.; Xu, Tao; Das, Mainak; Gregory, Cassie; Hickman, J J; Boland, Thomas

doi:10.1016/j.biomaterials.2003.10.052

Cited by 648 publications

(438 citation statements)

References 30 publications

Supporting

Mentioning

430

Contrasting

Unclassified

Order By: Relevance

“…Many cell types have been successfully patterned with microfluidics [14][15][16], lCP [17], inkjet printing [18,19], plasma treatment [20], self-assembled monolayers [21][22][23][24], self-assembled constructs [25], laser scanning lithography [26], atomic force microscope lithography, dip-pen nanolithography [27], topography [28,29], carbon nano-tubes [30], or their combinations [31,32]. Neurons are, however, distinctive cells with highly polarized morphology, much smaller somata, and thus few anchoring points for adhesion in comparison to most types of adherent mammalian cells.…”

Section: Introductionmentioning

confidence: 99%

Surface Coating as a Key Parameter in Engineering Neuronal Network Structures In Vitro

et al. 2012

View full text Add to dashboard Cite

By quantitatively comparing a variety of macromolecular surface coating agents, we discovered that surface coating strongly modulates the adhesion and morphogenesis of primary hippocampal neurons and serves as a switch of somata clustering and neurite fasciculation in vitro. The kinetics of neuronal adhesion on poly-lysine-coated surfaces is much faster than that on laminin and Matrigel-coated surfaces, and the distribution of adhesion is more homogenous on poly-lysine. Matrigel and laminin, on the other hand, facilitate neuritogenesis more than poly-lysine does. Eventually, on Matrigel-coated surfaces of self-assembled monolayers, neurons tend to undergo somata clustering and neurite fasciculation. By replacing coating proteins with cerebral astrocytes, and patterning neurons on astrocytes through self-assembled monolayers, microfluidics and micro-contact printing, we found that astrocyte promotes soma adhesion and astrocyte processes guide neurites. There, astrocytes could be a versatile substrate in engineering neuronal networks in vitro. Besides, quantitative measurements of cellular responses on various coatings would be valuable information for the neurobiology community in the choice of the most appropriate coating strategy.

show abstract

Section: Introductionmentioning

confidence: 99%

Surface Coating as a Key Parameter in Engineering Neuronal Network Structures In Vitro

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Recent reports have shown that an inkjet printer can be modified to put live cells on to a substrate [7,8]. Xu et al [8] printed two different kinds of mammalian cells, an epithelial cell line [CHO (Chinese-hamster ovary) cells] and rat embryonic motoneurons, with good viability through the printing process.…”

Section: New Approaches Control the Placement Of Living Cellsmentioning

confidence: 99%

New ways to print living cells promise breakthroughs for engineering complex tissues in vitro

Withers

2006

Biochemical Journal

View full text Add to dashboard Cite

The ability to control the placement of cells and the assembly of networks in vitro has tremendous potential for understanding the regulation of development as well as for generating artificial tissues. To date, most engineering tools that can place materials with precision are not compatible with the requirements of living cells, and so approaches to tissue engineering have focused on patterning substrates as a way of controlling cell growth rather than patterning cells directly. In this issue of Biochemical Journal, however, Eagles et al. adapt electrohydrodynamic printing technology to 'print' living cells from a neuronal cell line on to a substrate. The importance of this approach is that it has the potential for unprecedented control over the position of cells in culture by directly placing them, thus allowing for the systematic assembly of cell networks.

show abstract

“…Formation of cell structures is important for the fundamental study of cell-cell communication and cell-based screening (Seliktar 2012). Recently, several methods have been investigated for cell patterning, such as soft lithography (Kane et al 1999;Kim et al 2012), laser-directed cell-writing (Odde and Renn 2000;Pasparakis et al 2011;Nahmias and Odde 2006), photolithographic techniques (Larsen et al 2014;Deguchi et al 2014), surface modification treatment (DeForest et al 2009;Yamazoe et al 2008), inkjet printing (Roth et al 2004) and optical-induced electrokinetic techniques (Liu et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Selective pattern of cancer cell accumulation and growth using UV modulating printing of hydrogels

Wei

et al. 2015

Biomed Microdevices

View full text Add to dashboard Cite

Fabrication of extracellular microenvironment for cancer cell growth in vitro is an indispensable technique to precisely control the cell spatial arrangement and proliferation for cell-behavior research. Current micropatterning methods usually require relatively complicated operations, which makes it difficult to investigate the effects of different cell growth patterns. This manuscript proposes a DMD-based projection technique to quickly pattern a poly(ethylene) glycol diacrylate (PEGDA)-based hydrogel on a common glass substrate. Using this method, we can effectively control the growth patterns of cells. Compared with these traditional methods which employ digital dynamic mask, polymerization of PEGDA solution can be used to create arbitrary shaped microstructures with high efficiency, flexibility and repeatability. The duration of UV exposure is less than 10 s through controlling the projected illumination pattern. The ability of patterned PEGDA-coated film to hinder cell adhesion makes it possible to control area over which cells attach. In our experiments, we take advantage of the blank area to pattern cells, which allows cells to grow in various pre-designed shapes and sizes. And the patterning cells have a high viability after culturing for several days. Interestingly, we found that the restricted space could stiffen and strengthen the cells. These results indicate that cells and extracellular microenvironment can influence each other.

show abstract

Inkjet printing for high-throughput cell patterning

Cited by 648 publications

References 30 publications

Surface Coating as a Key Parameter in Engineering Neuronal Network Structures In Vitro

Surface Coating as a Key Parameter in Engineering Neuronal Network Structures In Vitro

New ways to print living cells promise breakthroughs for engineering complex tissues in vitro

Selective pattern of cancer cell accumulation and growth using UV modulating printing of hydrogels

Contact Info

Product

Resources

About