Injury and Protection in Split-Thickness Skin after Very Rapid Cooling and Warming

Zieger, Michael; Tredget, Edward E.; Sykes, Brian D.; McGann, Locksley E.

doi:10.1006/cryo.1997.2025

Cited by 20 publications

(12 citation statements)

References 28 publications

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“…The employed mechanism is similar to that of vitrification, although lower concentrations are used. (57,58) By perturbing sperm structure and metabolism, however, glycerol seems to produce a contraceptive action when used for the cryopreservation of chicken sperm, and this may apply to other species. This effect would be partly generated by a spermatozoon membrane osmotic disequilibrium.…”

Section: Industrial Applicationsmentioning

confidence: 99%

Glycerol: a neglected variable in metabolic processes?

et al. 2001

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Glycerol is a small and simple molecule produced in the breakdown of glucose, proteins, pyruvate, triacylglycerols and other glycerolipid, as well as release from dietary fats. An increasing number of observations show that glycerol is probably involved in a surprising variety of physiopathologic mechanisms. Glycerol has long been known to play fundamental roles in several vital physiological processes, in prokaryotes and eukaryotes, and is an important intermediate of energy metabolism. Despite some differences in the details of their operation, many of these mechanisms have been preserved throughout evolution, demonstrating their fundamental importance. In particular, glycerol can control osmotic activity and crystal formation and then act as a cryoprotective agent. Furthermore, its properties make it useful in numerous industrial, therapeutic and diagnostic applications. Few studies have focussed directly on glycerol, however, and while its metabolism is increasingly well documented, much of the details remain unknown. Considering the importance of glycerol in multiple vital physiological processes, its study could help unlock important physiopathological mechanisms.

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Section: Industrial Applicationsmentioning

confidence: 99%

Glycerol: a neglected variable in metabolic processes?

et al. 2001

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“…In case of snap frozen, glassy solids are formed inside and outside of the cells, and quick rewarming can avoid "devitrification" during temperature rise process. "Devitrification"means glassy water or tiny ice crystals-bearing solid water is recrystallized prior to thawing, or tiny ice crystals are rethawed into large ice crystals, which destroy cell structures and cause cell death (Zieger et al, 1997).…”

Section: Thermal Physics Of Skin Low-temperature Preservationmentioning

confidence: 99%

Skin Graft Preservation

Ge¹,

Huang²,

Wei³

2011

Skin Grafts - Indications, Applications and Current Research

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“…These grafts are of either bovine 44,45 or human origin. [46][47][48] Two publications concern samples of isolated keratinocytes 44 and microsurgical skin strips with their nutritive vascular pedicle, through which cryopreservatives were administered.…”

Section: Cryopreservation Of Cutaneous Tissuementioning

confidence: 99%

“…During slow thawing, they may aggregate medium. 45 Keratinocytes in the basal layer were the last to be impregnated.…”

mentioning

confidence: 99%