Injurious effects of oxygen free radicals on human intestinal epithelial cells and their prevention

Liao, Xian-ping; She, Ya-xiong; Liu, Shanlin; Li, Min

doi:10.1007/bf00171164

Cited by 3 publications

(2 citation statements)

References 10 publications

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“…Besides, obstructive jaundice weakens anti-oxidant defenses and leads to increased free radicals (3). Moreover, overproduction of oxygen derived free radicals correlates with impaired gut barrier and increased BT (4,5).…”

Section: Introductionmentioning

confidence: 99%

The Effects of Nitric Oxide Supplementation and Inhibition on Bacterial Translocation in Bile Duct Ligated Rats

Demirkiran

Balkaya

Tuncyurek

et al. 2006

Acta Chirurgica Belgica

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Obstructive jaundice promotes bacterial translocation from the gut, but the role of nitric oxide is controversial in this process. We studied the effects of nitric oxide synthase substrate, L-arginine, and nitric oxide synthase inhibitor, N(G)-nitro-L-arginine methyl ester, on bacterial translocation in bile duct ligated rats. The animals were randomized into five groups; control, sham, common bile duct ligation alone, nitric oxide inhibition, and nitric oxide supplementation. Obstructive jaundice was performed with common bile duct ligation. L-arginine or N(G)-nitro-L-arginine methyl ester was injected once daily for 14 days. Blood bilirubin level, liver histology, and bacterial translocation to the mesenteric lymph nodes as well as to the liver were assessed. The L-arginine supplemented group had the lowest bacterial translocation rate, but the most prominent hepatic fibrosis. Nitric oxide inhibition increased bacterial translocation to the mesenteric lymph nodes. Therefore, the administration of nitric oxide donor or inhibitor acts as a significant regulatory factor for bacterial translocation in obstructive jaundice.

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Section: Introductionmentioning

confidence: 99%

The Effects of Nitric Oxide Supplementation and Inhibition on Bacterial Translocation in Bile Duct Ligated Rats

Demirkiran

Balkaya

Tuncyurek

et al. 2006

Acta Chirurgica Belgica

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“…Furthermore, bile duct ligation (BDL) reduces antioxidant defenses and attenuates free radical formation [7] . It has been reportedly demonstrated that there is a strong correlation between BT and formation of free radicals, via increased lipid peroxidation or decreased scav-enging mechanism in the intestinal mucosa [8,9] . The role which nitric oxide (NO) plays in this process is controversial: NO reacts with superoxide anion to form a hydroxyl radical and peroxynitrite which are potent sources of oxidative injury [10,11] .…”

Section: Introductionmentioning

confidence: 99%

Does Pharmaconutrition with <i>L</i>-Arginine and/or α-Tocopherol Improve the Gut Barrier in Bile Duct Ligated Rats?

Tuncyurek

Sarı²,

Fırat³

et al. 2006

Eur Surg Res

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Background/Aim: Nitric oxide supplementation and antioxidant therapy modulate gut barrier function, but the relationships between enhanced nitric oxide production, antioxidant administration, and biliary obstruction remain unclear. We evaluated the role of nitric oxide and α-tocopherol supplementation in bile duct ligated rats. Methods: Fifty male Wistar albino rats underwent sham operation (group I; control animals) or bile duct ligation (groups II, III, IV, and V). The ligation groups received the following regimens: standard pellet diet (group II), pellet diet plus intramuscularly administerd α-tocopherol (group III), and L-arginine-enriched pellet diet without (group IV) or with (group V) α-tocopherol. Nitric oxide, malondialdehyde, and α-tocopherol concentrations were assessed at the end of 3 weeks. Liver and intestinal samples were scored histologically. Mesenteric lymph node and liver cultures were assessed for bacterial translocation. Results: The liver malondialdehyde concentration was highest in group III. The nitric oxide content in the liver was higher in groups III and V, as were the blood α-tocopherol levels. Bacterial translocation was evident following bile duct ligation, but did not differ among the treatment groups. Intestinal histology revealed that group III had the lowest villus height, that group V had the least villus count, and that group II had the highest mucous cell count. The fibrosis scores were higher in groups IV and V. Conclusions: An obvious effect of α-tocopherol (with or without L-arginine) on the gut barrier could not be demonstrated. Moreover, the L-arginine-enriched diet promoted fibrosis in the liver. Thus, while biliary duct obstruction triggers bacterial translocation, nitric oxide and/or α-tocopherol supplementation did not seem to improve the gut barrier in our model.

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Effects of the anti-ICAM-1 monoclonal antibody, allopurinol, and methylene blue on intestinal reperfusion injury

İlhan¹,

Alataş²,

Tokar³

et al. 2003

Journal of Pediatric Surgery

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Injurious effects of oxygen free radicals on human intestinal epithelial cells and their prevention

Cited by 3 publications

References 10 publications

The Effects of Nitric Oxide Supplementation and Inhibition on Bacterial Translocation in Bile Duct Ligated Rats

The Effects of Nitric Oxide Supplementation and Inhibition on Bacterial Translocation in Bile Duct Ligated Rats

Does Pharmaconutrition with <i>L</i>-Arginine and/or α-Tocopherol Improve the Gut Barrier in Bile Duct Ligated Rats?

Effects of the anti-ICAM-1 monoclonal antibody, allopurinol, and methylene blue on intestinal reperfusion injury

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