Injuries Induced by Diffuse Photodynamic Action in Retina and Choroid of Albino Rats

Büchi, Ernst R.; Lam, Tsun Ip; Suvaizdis, Irena; Tso, Mark O.M.

doi:10.1097/00006982-199414040-00015

Cited by 13 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Choroidal involvement, characterized by both choriocapillary occlusion and non-occlusion injury, is deemed a necessity for the development of exudative RD [ 38 ]. Additionally, RPE injury can occur independently with choriocapillary ischemia or in combination with photodynamic free radical injury [ 39 ]. Either choriocapillary occlusion alone or RPE injury combined with non-occlusion choriocapillary injury can lead to the formation of serous RD [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Exploring laser-induced acute and chronic retinal vein occlusion mouse models: Development, temporal in vivo imaging, and application perspectives

Xu,

Li,

Tang

et al. 2024

PLoS ONE

View full text Add to dashboard Cite

Photodynamic venous occlusion is a commonly accepted method for establishing mouse models of retinal vein occlusion (RVO). However, existing model parameters do not distinguish between acute and chronic RVO subtypes. Large variations in laser energy seem to correlate with fluctuating retinopathy severity and high rates of venous recanalization during the acute phase, along with the variable levels of retinal perfusion during the chronic phase. After optimizing the modeling procedure and defining success and exclusion criteria, laser energy groups of 80mW, 100mW, and 120mW were established. Multimodal imaging confirmed that higher energy levels increased the incidence of retinal cystoid edema and intraretinal hemorrhage, exacerbated the severity of exudative retinal detachment, and reduced the venous recanalization rate. For the acute model, 100mW was considered an appropriate parameter for balancing moderate retinopathy and venous recanalization. Continuous imaging follow-up revealed that day 1 after RVO was the optimal observation point for peaking of retinal thickness and intensive occurrence of retinal cystic edema and intraretinal hemorrhage. After excluding the influence of venous recanalization on retinal thickness, acute retinal edema demonstrated a positive response to standard anti-vascular endothelial growth factor therapy, validating the clinical relevance of the acute RVO model for further study in pathogenic mechanisms and therapeutic efficacy. For the chronic model, the 120mW parameter with the lowest venous recanalization rate was applied, accompanied by an increase in both photocoagulation shots and range to ensure sustained vein occlusion. Imaging follow-up clarified non-ischemic retinopathy characterized by tortuosity and dilation of the distal end, branches, and adjacent veins of the occluded vein. These morphological changes are quantifiable and could be combined with electrophysiological functional assessment for treatment effectiveness evaluation. Moreover, the stable state of venous occlusion may facilitate investigations into response and compensation mechanisms under conditions of chronic retinal hypoperfusion.

show abstract

Section: Discussionmentioning

confidence: 99%

Exploring laser-induced acute and chronic retinal vein occlusion mouse models: Development, temporal in vivo imaging, and application perspectives

Xu,

Li,

Tang

et al. 2024

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…Miller et al (1994) used this same method in a non-human primate model, cynomolgus monkey ( Macaca fascicularis ), where a laser at 577 nm was used for causing occlusion. Buchi et al (1994) used this model to study morphological and histological changes in retina. Schimdt-Kastner also used Photothrombosis to induce ischemia in rat retina and showed the ganglion cell death (Schmidt-Kastner and Eysel, 1994).…”

Section: Photothrombosis Of Retinal Vesselsmentioning

confidence: 99%

Preclinical models to investigate retinal ischemia: advances and drawbacks

2012

View full text Add to dashboard Cite

Retinal ischemia is a major cause of blindness worldwide. It is associated with various disorders such as diabetic retinopathy, glaucoma, optic neuropathies, stroke, and other retinopathies. Retinal ischemia is a clinical condition that occurs due to lack of appropriate supply of blood to the retina. As the retina has a higher metabolic demand, any hindrance in the blood supply to it can lead to decreased supply of oxygen, thus causing retinal ischemia. The pathology of retinal ischemia is still not clearly known. To get a better insight into the pathophysiology of retinal ischemia, the role of animal models is indispensable. The standard treatment care for retinal ischemia has limited potential. Transplantation of stem cells provide neuroprotection and to replenish damaged cells is an emerging therapeutic approach to treat retinal ischemia. In this review we provide an overview of major animal models of retinal ischemia along with the current and preclinical treatments in use.

show abstract

“…As well, the lesion size and location can be modulated by altering the irradiating intensity, duration of light exposure, beam position and dye concentration [20,25] . However, this method produces variable histologic injury, which is difficult to quantify [26] , and it may also cause secondary damage due to neurotoxicity in addition to ischemic damage [25] . It is also worth noting that it is not an ideal method for the study of ischemia/reperfusion injury as the lesion created is resistant to the reintroduction of blood flow [25] .…”

Section: Animal Models Of Retinal Ischemiamentioning

confidence: 99%

What can we learn about stroke from retinal ischemia models?

D'Onofrio

Koeberle

2012

Acta Pharmacol Sin

View full text Add to dashboard Cite

Retinal ischemia is a very useful model to study the impact of various cell death pathways, such as apoptosis and necrosis, in the ischemic retina. However, it is important to note that the retina is formed as an outpouching of the diencephalon and is part of the central nervous system. As such, the cell death pathways initiated in response to ischemic damage in the retina reflect those found in other areas of the central nervous system undergoing similar trauma. The retina is also more accessible than other areas of the central nervous system, thus making it a simpler model to work with and study. By utilizing the retinal model, we can greatly increase our knowledge of the cell death processes initiated by ischemia which lead to degeneration in the central nervous system. This paper examines work that has been done so far to characterize various aspects of cell death in the retinal ischemia model, such as various pathways which are activated, and the role neurotrophic factors, and discusses how these are relevant to the treatment of ischemic damage in both the retina and the greater central nervous system.

show abstract

Injuries Induced by Diffuse Photodynamic Action in Retina and Choroid of Albino Rats

Cited by 13 publications

References 0 publications

Exploring laser-induced acute and chronic retinal vein occlusion mouse models: Development, temporal in vivo imaging, and application perspectives

Exploring laser-induced acute and chronic retinal vein occlusion mouse models: Development, temporal in vivo imaging, and application perspectives

Preclinical models to investigate retinal ischemia: advances and drawbacks

What can we learn about stroke from retinal ischemia models?

Contact Info

Product

Resources

About