Injektabel Komposit Hydroksiapatit-Gelatin sebagai Sistem Penghantaran Alendronat

Abstract

Background: Bisphosphonates, such as alendronate (ALE), have been known to be effective in the treatment of bone cancer and osteoporosis. However, it has been reported that the systemic administration of ALE causes a considerable side effect. Thus, the formulation injectable bone substitute (IBS) for local administration of ALE, which functions as drug delivery system (DDS) as well as filling agent in osteoporosis-induced bone fracture, is needed. Objective: To establish the biodegradable and biocompatible formulation for ALE in injectable form which supports the drug delivery system and acts as filling agent in bone fracture. Methods: Hydroxyapatite (HA) was added to the mixture of gelatin and hydroxypropyl methyl cellulose (GEL-HPMC). ALE was added to the mixture and semisolid form was prepared for granulation. The dried granule, as injectable matrix, was grinded and mixed with appropriate amount of Na2HPO4. Results: Porosity of injectable form was higher than those of granule form. Injectable semisolid form was produced by adding 0.8 mL Na2HPO4 on each gram of granule with 10-12 min setting time. MTT assay showed that matrix was biocompatible showed by more than 100% viability. In vitro dissolution study showed that ALE was slowly released in more than 20 days. Conclusions: The formula of IBS using HA-GEL-HPMC may act as an effective drug delivery system for local administration of ALE in bone fracture. AbstrakLatar belakang: Golongan bisfosfonat sebagai obat kelainan tulang sudah lama dipergunakan seperti kanker tulang dan osteoporis. Namun karena efek samping dari obat tersebut maka dibuat formula lokal dalam bentuk injektabel yang berfungsi sebagai sistem penghantaran obat dan pengisi celah yang terbentuk dari fraktur akibat osteoporosis. Tujuan: untuk membuat formula dari hidroksiapatit (HA) dan gelatin (GEL) sebagai penghantaran/pembawa obat (SPO) maupun pengisi tulang. Formula injektabel bersifat biodegradable dan biokompatibel dan obat yang digunakan adalah alendronat (ALE). Hidroksi propil Metil Selulosa (HPMC) ditambahkan untuk memudahkan pembawa membentuk bahan setengah padat. Metode: HA ditambahkan ke dalam campuran GEL-HPMC diaduk selajutnya di tambahkan ALE dan diaduk sampai membentuk massa yang tepat untuk dibuat granul. Granul kering digerus sebagai matrik injektabel dan diencerkan dengan Na2HPO4. Hasil: porositas (SEM) bentuk granul < bentuk hasil injeksi, repasta dari matrik bentuk serbuk memerlukan Na2HPO4 0,8 ml/gram dan seting dalam waktu 10-12 menit. Matrik bersifat biokompatibel dengan viabilitas >100% (MTT). Dari hasil uji disolusi in vitro menunjukkan bahwa ALE dapat dilepas secara perlahan dalam waktu > 20 hari. Kesimpulan: Formula injektabel (IBS) HA-GEL-HPMC dapat berfungsi sebagai penghantaran ALE secara lokal. Kata kunci: hidroksi apatit, Gelatin, HPMC, alendronat, Na2HPO4, sistem penghantaran obat

Jelly Candy Hydroxyapatite from Mackerel Fish Bone

“…The development of science and technology in the field of pharmacy encourages pharmacists to make the right formulations to process hydroxyapatite into a form of preparation that is easily accepted by the community (Andasari, Zukhri and Nurjanah, no date). Pharmaceutical preparations made from hydroxyapatite active that has been made into a pharmaceutical product are the form of toothpaste (Anggresani et al, 2021;Hernawan et al, 2021;Wadu et al, 2015), biomaterials (Hanura et al, 2017), and injectable bone substitutes (Budiatin et al, 2016). There are also jelly preparations made from active calcium (Lesmana et al, 2008) and there are preparations of calcium jelly candy in the market.…”

Hydroxyapatite (HAp) From Tenggiri Fish Bones As Abrasive Material In Toothpaste Formula

“…The hydroxyapatite has been used in the pharmacy fields such as tablet making (Mustaqim, 2017), dental implant (Sidiqa et al, 2012), injection manufacture (Budiatin et al, 2016). However, there was not an investigation on making toothpaste from hydroxyapatite tenggiri fish bone, hence this studyis conducted to investigate hydroxyapatite toothpaste that can cover the holes in the teeth.…”