Injection site‐dependent induction of immune response by DNA vaccine: comparison of skin and spleen as a target for vaccination

Abstract

The results obtained in the present study indicate that the spleen is unlikely to be a good target for immunization despite the presence of a large number of lymphocytes and efficient production of transgenes.

Injectable, self-gelling, biodegradable, and immunomodulatory DNA hydrogel for antigen delivery

“…Measurement of OVA-specific antibody. Serum samples were serially diluted to measure the OVA-specific total IgG levels by ELISA as previously described [23].…”

The impact of intraoperative vaccination with IL-12 modified autologous tumor cells in the Lewis lung carcinoma mouse model

“…immunization is still discussed controversially (Cayeux et al 2001;Guan et al 2010). Anti-tumor DNA or RNA vaccines administered i.s.…”

Comparison of antigen expression from plasmid DNA in tumor-free and antigen-expressing tumor-bearing mice

“…Seven days after the last immunization, serum samples were collected from the tail vein of mice to measure anti-OVA IgG antibodies by an enzymelinked immunosorbent assay (ELISA) as described previously. 3,4 In brief, 96-well flat-bottom polystyrene plate was coated with OVA (1 mg/ml) by overnight incubation at 4°C. The wells were blocked with 5% BSA-containing phosphate buffered saline with Tween-20 (0.5 w/w% Tween-20 in PBS).…”

“…Seven days after the last immunization, spleens were isolated from the immunized mice and cytotoxic T lymphocyte (CTL) assay was performed as described previously. 3,4 In brief, spleen cells were restimulated with mitomycin C-treated EG7-OVA for 5 d in vitro. Target cells, EG7-OVA, were labeled with 51 Cr by incubating with Na 2 51 CrO 4 (Daiichi Radioisotope Labs, Tokyo, Japan).…”

“…2 Immunization of naïve mice with DNA vaccines generally induces antigen-specific immune responses, which is strong enough to reject tumor challenge. 3,4 However, high prophylactic effects of DNA vaccines do not guarantee their therapeutic effects in established cancer.…”