Comparison of antigen expression from plasmid DNA in tumor-free and antigen-expressing tumor-bearing mice

Removal of transgene-expressing cells by a specific immune response induced by sustained transgene expression

“…Serum samples were obtained as described above. fLuc‐specific antibodies were measured using an enzyme‐linked immunosorbent assay (ELISA), as described previously . In brief, 96‐well flat‐bottom polystyrene plates were coated with 0.2 mg/ml of fLuc (Promega, San Luis Obispo, CA, USA) followed by overnight incubation at 4°C.…”