Injectable Self‐Healing Antibacterial Bioactive Polypeptide‐Based Hybrid Nanosystems for Efficiently Treating Multidrug Resistant Infection, Skin‐Tumor Therapy, and Enhancing Wound Healing

Abstract

The surgical procedure in skin-tumor therapy usually results in cutaneous defects, and multidrug-resistant bacterial infection could cause chronic wounds. Here, for the first time, an injectable self-healing antibacterial bioactive polypeptide-based hybrid nanosystem is developed for treating multidrug resistant infection, skin-tumor therapy, and wound healing. The multifunctional hydrogel is successfully prepared through incorporating monodispersed polydopamine functionalized bioactive glass nanoparticles (BGN@ PDA) into an antibacterial F127-ε-Poly-L-lysine hydrogel. The nanocomposites hydrogel displays excellent self-healing and injectable ability, as well as robust antibacterial activity, especially against multidrug-resistant bacteria in vitro and in vivo. The nanocomposites hydrogel also demonstrates outstanding photothermal performance with (near-infrared laser irradiation) NIR irradiation, which could effectively kill the tumor cell (>90%) and inhibit tumor growth (inhibition rate up to 94%) in a subcutaneous skin-tumor model. In addition, the nanocomposites hydrogel effectively accelerates wound healing in vivo. These results suggest that the BGN-based nanocomposite hydrogel is a promising candidate for skin-tumor therapy, wound healing, and antiinfection. This work may offer a facile strategy to prepare multifunctional bioactive hydrogels for simultaneous tumor therapy, tissue regeneration, and anti-infection.

An injectable photopolymerized hydrogel with antimicrobial and biocompatible properties for infected skin regeneration

“…ε-PL causes physiological damage to cells and reduces microbial resistance by the adsorption through microbial membranes 29 . ε-PL can be used in wound dressings to effectively treat bacterial infections 30,31 . γ-Poly(glutamic acid) (γ-PGA) is a poly-amino acid produced by microbial fermentation that consists of a high-molecular-weight polymer, with glutamic acid units that form peptide bonds through α-amino and γ-carboxyl groups.…”

Tetracycline hydrochloride loaded citric acid functionalized chitosan hydrogel for wound healing

“…1,2 Hydrogels, three-dimensional crosslinked hydrophilic polymers with a very high intrinsic content of water, promote moist healing, are non-adherent and cool the surface of the wound, which may lead to a marked reduction in pain; they therefore have high patient acceptability and are suitable as wound dressings for wound healing. [3][4][5][6][7] Currently, many different materials such as poly(vinyl alcohol) (PVA), poly(ethylene glycol) (PEG), polyvinylpyrrolidone (PVP), poly(methacrylates) (PMMA), carboxymethyl cellulose (CMC) and alginate have been used as hydrogel dressing materials. 1,[8][9][10][11] Chitosan is considered to be one of the most widely used nature biopolymer for biomedical applications, especially in the eld of wound dressings due to its favorable biological activities such as hemostasis, biocompatibility, biodegradability and antimicrobial properties.…”

Advances in Synthesis and Applications of Self-Healing Hydrogels

“…Those networks can be mainly divided into two cross-linking types: (1) dynamic chemical covalent bonds and (2) physical non-covalent interactions. Dynamic covalent bonds include imine bond ( Qu et al, 2017 ; Yan et al, 2017 ; Chen H. et al, 2018 ; Chen et al, 2019e ; Khan et al, 2018 ; Wei and Gerecht, 2018 ; Zhang et al, 2018 ; Guo B. et al, 2019 ; Jiang F. et al, 2019 ; Li Q. et al, 2019 ; Qian et al, 2019 ; Wang et al, 2019b ; Wang W. et al, 2019 ; Zhou et al, 2019 ), Diels–Alder (DA) reaction ( Oehlenschlaeger et al, 2014 ), boronate-diol complexation ( Hong et al, 2018 ; Pan et al, 2018 ; Smithmyer et al, 2018 ; Zhi et al, 2018 ; Han et al, 2019 ), acylhydrazone bond ( Yang et al, 2017 ; Shen et al, 2019 ; Sun C. et al, 2019 ), dynamic disulfide bond ( Song L. et al, 2019 ), and dynamic metal oordination ( Shao et al, 2018 ; Chen et al, 2019a ; Qin et al, 2019 ). Physical non-covalent interactions include hydrogen bonding ( Fan et al, 2018 ; Uzumcu et al, 2018 ; Cao et al, 2019 ; Chen et al, 2019c ; Hu et al, 2019 ; Huang et al, 2019 ; Kuddushi et al, 2019 ; Li R. et al, 2019 ; Liao et al, 2019 ; Su et al, 2019 ; Wang L. et al, 2019 ), hydrophobic interaction ( Liu P. et al, 2019 ), host-guest interaction ( Deng Z. et al, 2018 ; Li Q. et al, 2018 ; Liu L. et al, 2019 ; Wang W. et al, 2019 ), and ionic interaction ( Ma et al, 2018 ; Wang Y. et al, 2018 ; Becher et al, 2019 ).…”