Injectable Polypeptide‐Protein Hydrogels for Promoting Infected Wound Healing

Abstract

Protein is the key composition of all tissues, which has also been widely used in tissue engineering due to its superior biocompatibility and low immunogenicity. However, natural protein usually lacks active functions such as vascularization, osteo‐induction, and neural differentiation, which limits its further applications as a functional biomaterial. Here, based on the mimetic extracellular matrix feature of bovine serum albumin, injectable polypeptide‐protein hydrogels with vascularization and antibacterial abilities are constructed successfully via coordinative cross‐linking of sulfydryl groups with silver ions (Ag+) for the regeneration of infected wound. In this protein hydrogel system, (Ag+), acting as crosslinkers, can not only provide a sterile microenvironment and a strong and robust antibacterial ability but also introduce K2(SL)6K2 (KK) polypeptide, which endows the hydrogel with vascularization behavior. Furthermore, the in vivo data show that the polypeptide‐protein hydrogel has a considerable collagen deposition and vascularization abilities in the early stage of wound healing, resulting in rapid new tissue regeneration featured with newly appeared hair follicles. Altogether, this newly developed multifunctional 3D polypeptide‐protein hydrogel with vascularization, antibacterial properties, and hair follicle promotion can be a promising approach in biomedical fields such as infected wound healing.

Bioinspired Functional Black Phosphorus Electrospun Fibers Achieving Recruitment and Biomineralization for Staged Bone Regeneration

“…Biocompatibility Test: CCK-8 was employed to analyze the proliferation behavior of primary rat BMSCs (10 000 per well) on the scaffolds, which was performed according to the protocol provided by the Dojindo company. [43] In brief, the test reagent was mixed with fresh Dulbecco's Modified Eagle Medium (DMEM, Gibco) on day 1, 3, and 5 and incubated for 2 h at a constant incubator containing 5% CO 2 . Then the mixed solution absorbance was quantified by a microplate reader (Infinite F50, TECAN) at 450 nm.…”

Pure-silk fibroin hydrogel with stable aligned micropattern toward peripheral nerve regeneration

“…Silk-based materials have shown a promising capability to sustain Schwann cell growth for nerve repair [43]. Previously, it was also found that polyacrylamide hydrogel with a stable and clear surface groove structure of 30 µm pave the way for effectively guiding the cell growth of Schwann cells [22]. Therefore, the behavior of Schwann cells on both TPSF hydrogel and Topo@TPSF hydrogel with a groove width of 30 µm was investigated.…”

“…Biocompatible materials, such as collagen [17], silk [18], and chitosan [19], have great potential in biomedical applications [20]. These natural biopolymers have significant advantages, such as long-term safety [21], desirable biocompatibility [22], and biodegradability [23]. However, the weak mechanical strength of most biopolymer-based scaffolds goes against the formation of artificial topographic structures [24].…”

Black phosphorus-based 2D materials for bone therapy

“…Taking advantage of the vascularization of vascular peptide, the 3D extracellular matrix characteristics of BSA protein and the rapid antibacterial ability of silver ions, the repair of infected wounds was promoted. In addition, hydrogels are also widely used in critical bone defects [ 152 ]. Yan et al prepared silk fibroin hydrogels with high cell adhesion function to repair bone defects by enhancing the osteogenic differentiation ability of bone marrow mesenchymal stem cells (BMSCs) [ 153 ].…”