Injectable nanoemulsions prepared by high pressure homogenization: processing, sterilization, and size evolution

Cappellani, Martina Rosi; Perinelli, Diego Romano; Pescosolido, Laura; Schoubben, Aurelie Marie Madeleine; Cespi, Marco; Cossi, Riccardo; Blasi, Paolo

doi:10.1007/s13204-018-0829-2

Cited by 23 publications

(12 citation statements)

References 43 publications

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“…Carlina acaulis EO-based NE was obtained through a high-energy method by using a high-pressure homogenizer. It was prepared according to the procedure reported by Rosi Cappellani et al [ 73 ]. Briefly, 6% ( w / w ) of C. acaulis EO was added dropwise to a 4% ( w / w ) of surfactant (Polysorbate 80, Sigma-Aldrich) aqueous solution under high-speed stirring (Ultraturrax T25 basic, IKA ® Werke GmbH and Co.KG, Staufen, Germany) for 5 min at 9500 rpm.…”

Section: Methodsmentioning

confidence: 99%

Developing a Highly Stable Carlina acaulis Essential Oil Nanoemulsion for Managing Lobesia botrana

et al. 2020

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The growing interest in the development of green pest management strategies is leading to the exploitation of essential oils (EOs) as promising botanical pesticides. In this respect, nanotechnology could efficiently support the use of EOs through their encapsulation into stable nanoformulations, such as nanoemulsions (NEs), to improve their stability and efficacy. This technology assures the improvement of the chemical stability, hydrophilicity, and environmental persistence of EOs, giving an added value for the fabrication of natural insecticides effective against a wide spectrum of insect vectors and pests of public and agronomical importance. Carlina acaulis (Asteraceae) root EO has been recently proposed as a promising ingredient of a new generation of botanical insecticides. In the present study, a highly stable C. acaulis-based NE was developed. Interestingly, such a nanosystem was able to encapsulate 6% (w/w) of C. acaulis EO, showing a mean diameter of around 140 nm and a SOR (surfactant-to-oil ratio) of 0.6. Its stability was evaluated in a storage period of six months and corroborated by an accelerated stability study. Therefore, the C. acaulis EO and C. acaulis-based NE were evaluated for their toxicity against 1st instar larvae of the European grapevine moth (EGVM), Lobesia botrana (Denis & Schiffermüller, 1775) (Lepidoptera: Tortricidae), a major vineyard pest. The chemical composition of C. acaulis EO was investigated by gas chromatography–mass spectrometry (GC–MS) revealing carlina oxide, a polyacetylene, as the main constituent. In toxicity assays, both the C. acaulis EO and the C. acaulis-based NE were highly toxic to L. botrana larvae, with LC50 values of 7.299 and 9.044 µL/mL for C. acaulis EO and NE, respectively. The C. acaulis-based NE represents a promising option to develop highly stable botanical insecticides for pest management. To date, this study represents the first evidence about the insecticidal toxicity of EOs and EO-based NEs against this major grapevine pest.

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Section: Methodsmentioning

confidence: 99%

Developing a Highly Stable Carlina acaulis Essential Oil Nanoemulsion for Managing Lobesia botrana

et al. 2020

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“…In the High Pressure Homogenization (HPH) method, an aqueous phase containing the emulsifier is added to an organic phase and ultraturrax is used to form an emulsion. Afterwards, the emulsion is added to the HPH in order to reduce droplet size [60]. Several homogenization cycles and pressures can be applied to obtain the desired nanoemulsion parameters.…”

Section: Nanoemulsions—a Brief Overviewmentioning

confidence: 99%

Current Applications of Nanoemulsions in Cancer Therapeutics

et al. 2019

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Nanoemulsions are pharmaceutical formulations composed of particles within a nanometer range. They possess the capacity to encapsulate drugs that are poorly water soluble due to their hydrophobic core nature. Additionally, they are also composed of safe gradient excipients, which makes them a stable and safe option to deliver drugs. Cancer therapy has been an issue for several decades. Drugs developed to treat this disease are not always successful or end up failing, mainly due to low solubility, multidrug resistance (MDR), and unspecific toxicity. Nanoemulsions might be the solution to achieve efficient and safe tumor treatment. These formulations not only solve water-solubility problems but also provide specific targeting to cancer cells and might even be designed to overcome MDR. Nanoemulsions can be modified using ligands of different natures to target components present in tumor cells surface or to escape MDR mechanisms. Multifunctional nanoemulsions are being studied by a wide variety of researchers in different research areas mainly for the treatment of different types of cancer. All of these studies demonstrate that nanoemulsions are efficiently taken by the tumoral cells, reduce tumor growth, eliminate toxicity to healthy cells, and decrease migration of cancer cells to other organs.

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“…NE formulations are good candidates for sterilization by filtration due to their small GS, which is less than the maximum nominal pore size of membrane filters used in this sterilization technique (0.22 or 220 nm). Filtration has been widely adopted as a sterilization method during the preparation of NE formulations specifically for thermolabile drugs; however, appropriate actions should be taken to avoid loss of solute by adsorption onto the filter, and to avoid the release of contaminants from the filter [ 50 ]. Furthermore, the aseptic filling of the sterilized solution is needed.…”

Section: Resultsmentioning

confidence: 99%

Design of Topical Ocular Ciprofloxacin Nanoemulsion for the Management of Bacterial Keratitis

et al. 2021

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Bacterial keratitis (BK) is a critical ocular infection that can lead to serious visual disability. Ciprofloxacin (CIP), moxifloxacin (MOX), and levofloxacin (LFX) have been accepted as monotherapies by the US Food and Drug Administration for BK treatment. CIP is available commercially at 0.3% w/v concentration as an ophthalmic solution and as an ointment for ocular delivery. Because of solubility issues at physiological pH, CIP precipitation can occur at the corneal surface post instillation of the solution dosage form. Consequently, the ocular bioavailability of CIP is reduced. The ointment dosage form is associated with side effects such as blurred vision, itching, redness, eye discomfort, and eye dryness. This study aimed to design a CIP loaded nanoemulsion (NE; CIP-NE) to facilitate drug penetration into the corneal layers for improved therapeutic outcomes as well as to overcome the drawbacks of the current commercial ophthalmic formulations. CIP-NE formulations were prepared by hot homogenization and ultrasonication, using oleic acid (CIP-O-NE) and Labrafac® Lipophile WL 1349 (CIP-L-NE) as the oily phase, and Tween® 80 and Poloxamer 188 as surfactants. Optimized CIP-NE was further evaluated with respect to in vitro release, ex vivo transcorneal permeation, and moist heat sterilization process, using commercial CIP ophthalmic solution as a control. Optimized CIP-O-NE formulation showed a globule size, polydispersity index, and zeta potential of 121.6 ± 1.5 nm, 0.13 ± 0.01, and −35.1 ± 2.1 mV, respectively, with 100.1 ± 2.0 % drug content and was spherical in shape. In vitro release and ex vivo transcorneal permeation studies exhibited sustained release and a 2.1-fold permeation enhancement, respectively, compared with commercial CIP ophthalmic solution. Autoclaved CIP-O-NE formulation was found to be stable for one month (last time-point tested) at refrigerated and room temperature. Therefore, CIP-NE formulation could serve as an effective delivery system for CIP and could improve treatment outcomes in BK.

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Injectable nanoemulsions prepared by high pressure homogenization: processing, sterilization, and size evolution

Cited by 23 publications

References 43 publications

Developing a Highly Stable Carlina acaulis Essential Oil Nanoemulsion for Managing Lobesia botrana

Developing a Highly Stable Carlina acaulis Essential Oil Nanoemulsion for Managing Lobesia botrana

Current Applications of Nanoemulsions in Cancer Therapeutics

Design of Topical Ocular Ciprofloxacin Nanoemulsion for the Management of Bacterial Keratitis

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