Injectable in situ dual-crosslinking hyaluronic acid and sodium alginate based hydrogels for drug release

Zhang, Yufan; Li, Xian; Zhong, Nan; Huang, Yuanlin; He, Kewen; Yu, Xu

doi:10.1080/09205063.2019.1618546

Cited by 36 publications

(17 citation statements)

References 39 publications

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“…Injectable hydrogels based on naturally occurring polymers have gained considerable attention because they are inherently non-inflammatory, biocompatible, non-immunogenic, and biodegradable (Bermejo-Velasco et al., 2018 ). Hyaluronic acid (HA) and its derivatives have been widely used in the clinic and applied to transport and release incorporated proteins and anticancer drugs (Zhang et al., 2019 ). Conjugates of HA are tunable and have better therapeutic efficacy than free anticancer drugs (Wang et al., 2019 ; Zhong et al., 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Intratumoral injection of anlotinib hydrogel enhances antitumor effects and reduces toxicity in mouse model of lung cancer

et al. 2020

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This study was conducted to determine the antitumor effects and ability of an anlotinib (AL) hydrogel (AL–HA–Tyr) to reduce toxicity in a mouse model of Lewis lung cancer (LLC). We constructed a drug carrier system for AL, verified its effectiveness and systemic safety, and provided a preliminary experimental foundation for clinical carrier transformation. AL–HA–Tyr was prepared by encapsulating AL with hyaluronic acid–tyramine (HA–Tyr) conjugates. Colony and tube formation assays showed that AL–HA–Tyr restrained the proliferation of human umbilical vein endothelial cells (HUVECs) and LLC cells, respectively, in vitro , and that AL exerted significant anti-angiogenesis and anti-tumor effects. The invasion and migration of HUVECs and LLC cells were efficiently suppressed by AL according to transwell assays. HUVEC and LLC cell-cycle and apoptosis analysis clarified the direct anti-tumor effects of AL–HA–Tyr. Mice engrafted with LLC cells in vivo were administered oral saline, oral AL, or an intratumoral injection of HA–Tyr or AL–HA–Tyr. The results showed that AL–HA–Tyr obviously reduced visceral toxicity and decreased Ki67 and VEGF-A expression in tumor cells compared with AL. Furthermore, AL–HA–Tyr significantly prolonged the survival of tumor-bearing mice. Overall, AL–HA–Tyr enhanced antitumor effects and reduced toxicity in the LLC model. It provided a foundation for the clinical transformation of drug carrier systems.

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Section: Introductionmentioning

confidence: 99%

Intratumoral injection of anlotinib hydrogel enhances antitumor effects and reduces toxicity in mouse model of lung cancer

et al. 2020

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“…The release rate of Endostar from EH was evaluated as described previously. 21 Two milliliters of EH was mixed with 1 mL of PBS, and shaken with a rotary shaker at 200 rpm and 37°C. At the predetermined intervals, 1 mL of supernatant was collected and equal volume of fresh buffer was added.…”

Section: Methodsmentioning

confidence: 99%

Synergy of Polydopamine Nanovaccine and Endostar Alginate Hydrogel for Improving Antitumor Immune Responses Against Colon Tumor

Yang¹,

Wang²,

Tian³

et al. 2022

IJN

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Background Tumor immunotherapy, a novel type of therapeutic treatment, has a wide range of applications with potentially prolonged benefits. However, current immunotherapy has a low overall response rate in treating a variety of tumors. Combination of immunotherapy with other therapies can improve the therapeutic response rates. The purpose of this work was to explore the potential of anti-angiogenic treatment in combination with tumor cell lysate loaded polydopamine nanoparticle vaccine as a therapeutic strategy for colon tumor. Methods We grafted tumor cell lysate onto polydopamine nanoparticles as nano-vaccine (TCLN) and fabricated alginate hydrogel loaded with Endostar (EH), then detected characteristics of EH and TCLN. We also estimated the cytotoxicity of EH/TCLN in vitro. In the tumor-bearing mouse model, we evaluated the antitumor effect of EH/TCLN treatment, and developed the animal survival study. After performing the EH/TCLN treatment, we also analyzed T cells and DCs using flow cytometry, and determined T cell responses and tumor microenvironmental cytokines. At last, we assessed the effect of the EH/TCLN treatment on anti-angiogenesis further. Results When applied in combination with TCLN in MC-38 tumor-bearing mice, EH/TCLN significantly suppressed tumor growth with more than half of the mice showing tumor regression. In addition, EH/TCLN treatment resulted in noticeable changes in the tumor microenvironment. As compared with the control group, EH/TCLN treatment led to significantly reduced tumor angiogenesis and expression of tumor microenvironment-related cytokines (TMCs), increased proportion of CD8 + T cells in the spleen, lymph node and tumor, elevated activity of cytotoxic T lymphocytes (CTLs) and tumor cell apoptosis. Conclusion The present study demonstrated that the EH/TCLN treatment effectively created a favorable immune microenvironment for the induction of antitumor immunity and improved antitumor immune responses.

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“…On-demand growth factor release could be obtained in these ferrogels upon magnetic field application as iron oxide nanoparticles movement causes alteration or disruption of hydrogel microstructure. On the other hand, Zhang Y et al [186] developed an in situ gelling alginate/HA hydrogel based on two types of covalent bonding: using oxidized alginate and modified HA containing thiol and hydrazide groups, covalent crosslinking formed via disulfide or hydrazone bonds between alginate and HA. These covalent alginate/GAG systems are a versatile strategy for dual-or multi-crosslinking hydrogels as they can be developed to enable the formation of covalent bonds of distinctive nature while still partially preserving alginate ability to ionic crosslink.…”

Section: Alginatementioning

confidence: 99%

Glycosaminoglycan-Inspired Biomaterials for the Development of Bioactive Hydrogel Networks

et al. 2020

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Glycosaminoglycans (GAG) are long, linear polysaccharides that display a wide range of relevant biological roles. Particularly, in the extracellular matrix (ECM) GAG specifically interact with other biological molecules, such as growth factors, protecting them from proteolysis or inhibiting factors. Additionally, ECM GAG are partially responsible for the mechanical stability of tissues due to their capacity to retain high amounts of water, enabling hydration of the ECM and rendering it resistant to compressive forces. In this review, the use of GAG for developing hydrogel networks with improved biological activity and/or mechanical properties is discussed. Greater focus is given to strategies involving the production of hydrogels that are composed of GAG alone or in combination with other materials. Additionally, approaches used to introduce GAG-inspired features in biomaterials of different sources will also be presented.

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Injectable in situ dual-crosslinking hyaluronic acid and sodium alginate based hydrogels for drug release

Cited by 36 publications

References 39 publications

Intratumoral injection of anlotinib hydrogel enhances antitumor effects and reduces toxicity in mouse model of lung cancer

Intratumoral injection of anlotinib hydrogel enhances antitumor effects and reduces toxicity in mouse model of lung cancer

Synergy of Polydopamine Nanovaccine and Endostar Alginate Hydrogel for Improving Antitumor Immune Responses Against Colon Tumor

Glycosaminoglycan-Inspired Biomaterials for the Development of Bioactive Hydrogel Networks

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